AB282871

Human MUC2 ELISA Kit

Recombinant
SimpleStep
What is this?

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(3 Publications)

Human MUC2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MUC2 with a sensitivity of 0.187 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

SMUC, MUC2, Mucin-2, MUC-2, Intestinal mucin-2

3 Images

ELISA

Supplier Data

ELISA - Human MUC2 ELISA Kit (AB282871)

sELISA

Supplier Data

Sandwich ELISA - Human MUC2 ELISA Kit (AB282871)

Interpolated concentrations of native MUC2 in human colon and bladder cancer based on 10 μg/mL and 100 µg/mL extract loads, respectively.

The concentrations of MUC2 were measured in duplicate and interpolated from the MUC2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MUC2 concentration was determined to be 27.28 ng/mL in colon tissue extract and 42.10 ng/mL in bladder cancer tissue extract.

sELISA

Supplier Data

Sandwich ELISA - Human MUC2 ELISA Kit (AB282871)

Example of human MUC2 standard curve in Sample Diluent NS.

The MUC2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell culture media, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.187 ng/mL

Range

0.78 - 50 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human MUC2 ELISA Kit ab282871 is a rapid single-wash 90-min Sandwich ELISA to measure Human MUC2 in cell culture media, serum, tissue extracts. This SimpleStep sensitivity is 0.187 ng/mL.

How the assay works

Human MUC2 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human MUC2 ELISA Kit ab282871 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) ab281111
- Anti-MUC2 antibody [EPR23479-91] - BSA and Azide free (Detector) ab281111

MUC2 coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. It is thought that MUC2 provides a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. MUC2 is a major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]

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Recovery

[ { "sample": "Serum", "range": "83 - 93 %", "average": "= 89" }, { "sample": "Tissue Extracts", "range": "93 - 105 %", "average": "= 98" }, { "sample": "Cell culture media", "range": "96 - 102 %", "average": "= 99" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Coats the epithelia of the intestines and other mucus membrane-containing organs to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces (PubMed : 17058067, PubMed : 19432394, PubMed : 33031746). Major constituent of the colon mucus, which is mainly formed by large polymeric networks of MUC2 secreted by goblet cells that cover the exposed surfaces of intestine (PubMed : 19432394, PubMed : 33031746). MUC2 networks form hydrogels that guard the underlying epithelium from pathogens and other hazardous matter entering from the outside world, while permitting nutrient absorption and gas exchange (PubMed : 33031746, PubMed : 36206754). Acts as a divalent copper chaperone that protects intestinal cells from copper toxicity and facilitates nutritional copper unptake into cells (PubMed : 36206754). Binds both Cu(2+) and its reduced form, Cu(1+), at two juxtaposed binding sites : Cu(2+), once reduced to Cu(1+) by vitamin C (ascorbate) or other dietary antioxidants, transits to the other binding site (PubMed : 36206754). MUC2-bound Cu(1+) is protected from oxidation in aerobic environments, and can be released for nutritional delivery to cells (PubMed : 36206754). Mucin gels store antimicrobial molecules that participate in innate immunity (PubMed : 33031746). Mucin glycoproteins also house and feed the microbiome, lubricate tissue surfaces, and may facilitate the removal of contaminants and waste products from the body (PubMed : 33031746). Goblet cells synthesize two forms of MUC2 mucin that differ in branched chain O-glycosylation and the site of production in the colon : a (1) 'thick' mucus that wraps the microbiota to form fecal pellets is produced in the proximal, ascending colon (By similarity). 'Thick' mucus transits along the descending colon and is lubricated by a (2) 'thin' MUC2 mucus produced in the distal colon which adheres to the 'thick' mucus (By similarity).

See full target information MUC2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 116:105721 PubMed40344717

2025

Faecal mucoprotein MUC2 is decreased in multiple sclerosis and is associated with mucin degrading bacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Luke A Schwerdtfeger,Federico Montini,Tanuja Chitnis,Laura M Cox,Howard L Weiner

Viruses 16: PubMed39066209

2024

Phage-Mediated Digestive Decolonization in a Gut-On-A-Chip Model: A Tale of Gut-Specific Bacterial Prosperity.

Applications

Unspecified application

Species

Unspecified reactive species

Brieuc Van Nieuwenhuyse,Maya Merabishvili,Nathalie Goeders,Kevin Vanneste,Bert Bogaerts,Mathieu de Jode,Joachim Ravau,Jeroen Wagemans,Leïla Belkhir,Dimitri Van der Linden

International journal of molecular sciences 25: PubMed38999957

2024

Effects of High-Mobility Group Box-1 on Mucosal Immunity and Epithelial Differentiation in Colitic Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Takamitsu Sasaki,Rina Fujiwara-Tani,Yi Luo,Ruiko Ogata,Rika Sasaki,Ayaka Ikemoto,Yukiko Nishiguchi,Chie Nakashima,Shingo Kishi,Kiyomu Fujii,Hitoshi Ohmori,Naohide Oue,Hiroki Kuniyasu

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human MUC2 ELISA Kit

ELISA - Human MUC2 ELISA Kit (AB282871)

Sandwich ELISA - Human MUC2 ELISA Kit (AB282871)

Sandwich ELISA - Human MUC2 ELISA Kit (AB282871)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (3)

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Product promise