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AB325621

Human Mucin 5AC ELISA Kit, Chemiluminescent - Extracellular

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Human Mucin 5AC ELISA Kit, Chemiluminescent - Extracellular is a 55 min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Mucin 5AC - Extracellular with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range : 2.29 - 5000 pg/ml with a sensitivity of 1.15 pg/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent pending

View Alternative Names

MUC5, MUC5AC, Mucin-5AC, MUC-5AC, Gastric mucin, Major airway glycoprotein, Tracheobronchial mucin, TBM

4 Images
Sandwich ELISA - Human Mucin 5AC ELISA Kit, Chemiluminescent - Extracellular (AB325621)
  • sELISA

Lab

Sandwich ELISA - Human Mucin 5AC ELISA Kit, Chemiluminescent - Extracellular (AB325621)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Mucin 5AC ELISA Kit, Chemiluminescent - Extracellular (AB325621)
  • sELISA

Lab

Sandwich ELISA - Human Mucin 5AC ELISA Kit, Chemiluminescent - Extracellular (AB325621)

Standard curve comparison between the Chemiluminescent Human Mucin 5AC SimpleStep ELISA and colorimetric Human Mucin 5AC SimpleStep ELISA (ab303761) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Mucin 5AC ELISA Kit, Chemiluminescent - Extracellular (AB325621)
  • sELISA

Lab

Sandwich ELISA - Human Mucin 5AC ELISA Kit, Chemiluminescent - Extracellular (AB325621)

Dynamic range comparison between the Chemiluminescent Human Mucin 5AC SimpleStep ELISA, colorimetric Human Mucin 5AC SimpleStep ELISA (ab303761), and leading chemiluminescent competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human Mucin 5AC ELISA Kit, Chemiluminescent - Extracellular (AB325621)
  • sELISA

Lab

Sandwich ELISA - Human Mucin 5AC ELISA Kit, Chemiluminescent - Extracellular (AB325621)

Interpolated concentration of native Mucin 5AC was measured in duplicate at different sample concentrations. Undiluted samples are 5% A549 supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.15 pg/mL

Range

2.29 - 5000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Mucin 5AC ELISA Kit, Chemiluminescent - Extracellular (ab325621) is a rapid single-wash sandwich 55 min ELISA to measure Human Mucin 5AC - Extracellular in Cell Culture Supernatant with a sensitivity of 1.15 pg/mL.

How the assay works

Human Mucin 5AC ELISA Kit, Chemiluminescent - Extracellular (ab325621) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Mucin 5AC - Extracellular (ab325621) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Mucin 5AC antibody [EPR24206-58] - BSA and Azide free (Capture) ab284953
- Anti-Mucin 5AC antibody [EPR24206-145] - BSA and Azide free (Detector) ab285059
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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.6" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "98 - 106 %", "average": "= 102" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325621-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325621-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Mucin 5AC Lyophilized Recombinant Protein", "number":"AB325621-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Mucin 5AC Detector Antibody", "number":"AB325621-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Mucin 5AC Capture Antibody", "number":"AB325621-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325621-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325621-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325621-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325621-CMP07", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325621-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB325621-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed : 14535999, PubMed : 14718370). Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed : 14535999).
See full target information MUC5AC
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com