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AB303761

Human Mucin 5AC ELISA Kit - Extracellular

5

(1 Review)

|

(3 Publications)

Human Mucin 5AC ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Human Mucin 5AC with a sensitivity of 3.461 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

MUC5, MUC5AC, Mucin-5AC, MUC-5AC, Gastric mucin, Major airway glycoprotein, Tracheobronchial mucin, TBM

3 Images
ELISA - Human Mucin 5AC ELISA Kit - Extracellular (AB303761)
  • ELISA

Supplier Data

ELISA - Human Mucin 5AC ELISA Kit - Extracellular (AB303761)
Sandwich ELISA - Human Mucin 5AC ELISA Kit - Extracellular (AB303761)
  • sELISA

Supplier Data

Sandwich ELISA - Human Mucin 5AC ELISA Kit - Extracellular (AB303761)

Interpolated concentrations of human Mucin 5AC in cell culture supernatant.

Interpolated concentration of native Mucin 5AC was measured in duplicate at different sample concentrations. Undiluted samples are 5% A549 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Mucin 5AC ELISA Kit - Extracellular (AB303761)
  • sELISA

Supplier Data

Sandwich ELISA - Human Mucin 5AC ELISA Kit - Extracellular (AB303761)

Example of human Mucin 5AC standard curve in Sample Diluent NS.

Example of human Mucin 5AC standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3.461 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Mucin 5AC ELISA Kit - Extracellular ab303761 is a rapid single-wash 90-min Sandwich ELISA to measure Human Mucin 5AC - Extracellularin cell culture supernatant. This SimpleStep sensitivity is 3.461 pg/mL.

How the assay works

Human Mucin 5AC - ExtracellularSimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Mucin 5AC ELISA Kit - Extracellular ab303761 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "98 - 106 %", "average": "= 102" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mucin 5AC also known simply as MUC5AC is a high molecular weight glycoprotein with a mass in the megadaltons typically found in the human respiratory gastrointestinal and reproductive tracts. It is a major component of mucus which plays a critical role in protecting and lubricating the epithelial surfaces of these organs. MUC5AC is primarily expressed in the goblet cells of the respiratory epithelium and the stomach lining. Due to its significance researchers often use a MUC5AC ELISA or other detection methods such as tagging with Alexa Fluor 555 to measure its levels in various tissues.
Biological function summary

The functions of MUC5AC are central to the maintenance of the protective mucus barrier on epithelial surfaces preventing pathogen invasion and facilitating the clearance of inhaled particles. It is a part of the gel-forming mucin family and often forms complex structures by polymerizing with other mucins like MUC2 and MUC5B contributing to the viscoelastic properties of mucus. These interactions ensure the formation of a stable and robust mucosal barrier.

Pathways

The regulation of MUC5AC production involves several signaling pathways including the EGFR (epidermal growth factor receptor) pathway and the IL-13/STAT6 pathway. These pathways influence mucin production and secretion particularly in response to growth factors and inflammatory cytokines. Proteins such as 13M1 and 11M1 interact with MUC5AC within these pathways modulating its expression and function. This regulation is essential for normal mucus homeostasis and response to environmental challenges.

MUC5AC has a strong association with respiratory conditions like asthma and chronic obstructive pulmonary disease (COPD). In these diseases the overproduction or altered composition of MUC5AC contributes to airway obstruction and reduced pulmonary function. MUC5AC levels can be indicative of disease severity and are often measured via tests like the 5AC blood test. In these contexts proteins such as 5AC protein and 45M1 are often studied for their roles in facilitating disease progression and as potential therapeutic targets to modulate mucin levels in affected individuals.
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Product protocols

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Target data

Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed : 14535999, PubMed : 14718370). Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed : 14535999).
See full target information MUC5AC
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 28:e70125 PubMed39365189

2024

KDELR2 is necessary for chronic obstructive pulmonary disease airway Mucin5AC hypersecretion via an IRE1α/XBP-1s-dependent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Wu,Fawang Du,Aijie Zhang,Guoyue Zhang,Rui Xu,Xianzhi Du

Viruses 16: PubMed39066209

2024

Phage-Mediated Digestive Decolonization in a Gut-On-A-Chip Model: A Tale of Gut-Specific Bacterial Prosperity.

Applications

Unspecified application

Species

Unspecified reactive species

Brieuc Van Nieuwenhuyse,Maya Merabishvili,Nathalie Goeders,Kevin Vanneste,Bert Bogaerts,Mathieu de Jode,Joachim Ravau,Jeroen Wagemans,Leïla Belkhir,Dimitri Van der Linden

International journal of chronic obstructive pulmonary disease 19:1635-1647 PubMed39045541

2024

Cigarette Smoke Extract Induces MUC5AC Expression Through the ROS/ IP3R/Ca Pathway in Calu-3 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Wu,Guoyue Zhang,Xianzhi Du
View all publications
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Product promise

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