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AB303762

Human Mucin 5AC ELISA Kit - Intracellular

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Human Mucin 5AC ELISA Kit - Intracellular is a single-wash 90-min Simplestep used to quantify Human Mucin 5AC with a sensitivity of 3.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

MUC5, MUC5AC, Mucin-5AC, MUC-5AC, Gastric mucin, Major airway glycoprotein, Tracheobronchial mucin, TBM

2 Images
Sandwich ELISA - Human Mucin 5AC ELISA Kit - Intracellular (AB303762)
  • sELISA

Supplier Data

Sandwich ELISA - Human Mucin 5AC ELISA Kit - Intracellular (AB303762)

Example of human Mucin 5AC standard curve in 1X Cell Extraction Buffer PTR.

Example of human Mucin 5AC standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Mucin 5AC ELISA Kit - Intracellular (AB303762)
  • sELISA

Supplier Data

Sandwich ELISA - Human Mucin 5AC ELISA Kit - Intracellular (AB303762)

Interpolated concentrations of human Mucin 5AC in cell and tissue extract.

Interpolated concentration of native Mucin 5AC was measured in duplicate at different sample concentrations. Undiluted samples are as follows : A549 extract 15 μg/mL and stomach extract 40 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3.7 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Mucin 5AC SimpleStep ELISA® kit (ab303762) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Mucin 5AC protein in cell extracts and tissue extracts. Quantitate Human Mucin 5AC with 3.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.1" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "83 - 83 %", "average": "= 83" }, { "sample": "Tissue Extracts", "range": "83 - 85 %", "average": "= 84" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab303762-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB303762-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB303762-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB303762-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB303762-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB303762-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB303762-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Mucin 5AC Capture Antibody", "number":"AB303762-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Mucin 5AC Lyophilized Recombinant Protein", "number":"AB303762-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Mucin 5AC Detector Antibody", "number":"AB303762-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB303762-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB303762-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mucin 5AC also known simply as MUC5AC is a high molecular weight glycoprotein with a mass in the megadaltons typically found in the human respiratory gastrointestinal and reproductive tracts. It is a major component of mucus which plays a critical role in protecting and lubricating the epithelial surfaces of these organs. MUC5AC is primarily expressed in the goblet cells of the respiratory epithelium and the stomach lining. Due to its significance researchers often use a MUC5AC ELISA or other detection methods such as tagging with Alexa Fluor 555 to measure its levels in various tissues.
Biological function summary

The functions of MUC5AC are central to the maintenance of the protective mucus barrier on epithelial surfaces preventing pathogen invasion and facilitating the clearance of inhaled particles. It is a part of the gel-forming mucin family and often forms complex structures by polymerizing with other mucins like MUC2 and MUC5B contributing to the viscoelastic properties of mucus. These interactions ensure the formation of a stable and robust mucosal barrier.

Pathways

The regulation of MUC5AC production involves several signaling pathways including the EGFR (epidermal growth factor receptor) pathway and the IL-13/STAT6 pathway. These pathways influence mucin production and secretion particularly in response to growth factors and inflammatory cytokines. Proteins such as 13M1 and 11M1 interact with MUC5AC within these pathways modulating its expression and function. This regulation is essential for normal mucus homeostasis and response to environmental challenges.

MUC5AC has a strong association with respiratory conditions like asthma and chronic obstructive pulmonary disease (COPD). In these diseases the overproduction or altered composition of MUC5AC contributes to airway obstruction and reduced pulmonary function. MUC5AC levels can be indicative of disease severity and are often measured via tests like the 5AC blood test. In these contexts proteins such as 5AC protein and 45M1 are often studied for their roles in facilitating disease progression and as potential therapeutic targets to modulate mucin levels in affected individuals.
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Product protocols

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Target data

Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed : 14535999, PubMed : 14718370). Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed : 14535999).
See full target information MUC5AC
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websiteProtocolBooklet
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Product promise

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