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AB108752

Human Anti-Mumps virus IgG ELISA Kit

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Human Anti-Mumps virus IgG ELISA Kit is an indirect ELISA for the qualitative detection of IgG class antibodies against Mumps virus in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Mumps virus IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Mumps virus in Human serum and plasma.

A 96-well plate has been precoated with Mumps virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Mumps virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mumps virus IgG sample captured in plate.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 7, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108752-1x96Tests", "assetComponentDetails": [ { "size":"1 x 2 mL", "name":"Mumps virus IgG Positive Control", "number":"AB108752-CMP04", "productcode":"" }, { "size":"1 x 3 mL", "name":"Mumps virus IgG Cut-off Control", "number":"AB108752-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Mumps virus (IgG) Coated Microplate (12 x 8 wells)", "number":"AB108752-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"Mumps virus anti-IgG HRP Conjugate", "number":"AB108752-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108752-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108752-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108752-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108752-CMP11", "productcode":"" }, { "size":"1 x 2 mL", "name":"Mumps virus IgG Negative Control", "number":"AB108752-CMP06", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgG Sample Diluent", "number":"AB108752-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108752-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mumps Virus IgG also known as IgG Mumps antibodies are immunoglobulins produced by the immune system in response to Mumps virus pathogen infection. These antibodies which have a molecular weight of approximately 150 kDa are found primarily in human blood serum. Mumps IgG can be detected and measured in serology tests to indicate a current or past infection or successful vaccination against the Mumps virus.
Biological function summary

Antibodies like Mumps IgG play a central role in immune response by neutralizing pathogens such as the Mumps virus and preventing their replication. They form part of a larger immune complex that identifies and marks the virus for destruction by other immune cells. The Mumps IgG antibodies persist in the body providing long-term immunity and serving as a marker for exposure in mumps testing and serology.

Pathways

The antibody-mediated immune response involves several signaling pathways including the activation of the complement system which enhances the ability of antibodies to clear pathogens. Proteins involved in these pathways include complement protein C1q which binds to antigen-antibody complexes signaling the recruitment of other complement proteins necessary for pathogen elimination.

Mumps Virus IgG antibodies are critical for the diagnosis and surveillance of mumps infection. Mumps a contagious viral infection can cause swelling of the salivary glands and other complications. The presence of IgG Mumps positive antibodies indicates immunity either from previous infection or vaccination thereby providing protective immunity. Other related conditions can include viral encephalitis where persistent Mumps virus presence can be connected to proteins involved in brain inflammation.
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Product protocols

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Target data

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websiteProtocolBooklet
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