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AB314357

Human Munc18-1 ELISA Kit

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Human Munc18-1 ELISA Kit is a Sandwich ELISA for the measurement of Human Munc18-1 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

UNC18A, STXBP1, Syntaxin-binding protein 1, MUNC18-1, N-Sec1, Protein unc-18 homolog 1, Protein unc-18 homolog A, p67, Unc18-1, Unc-18A

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Sandwich ELISA - Human Munc18-1 ELISA Kit (AB314357)
  • sELISA

Supplier Data

Sandwich ELISA - Human Munc18-1 ELISA Kit (AB314357)

Example of standard curve using ab314357. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.75 ng/mL

Range

0.75 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB124920

Anti-Munc18-1 antibody [EPR4850]

KO Validated|RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-Munc18-1 antibody [EPR4850] (AB124920)

  • 4
  • 1
Cell Lines & Lysates

AB94272

Munc18-1 overexpression 293T lysate (whole cell)

Western blot - Munc18-1 overexpression 293T lysate (whole cell) (AB94272)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Munc18-1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Munc18-1 in serum (human Munc18-1 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma (collect plasma using EDTA or citrate as an anticoagulant. Heparin is not recommended as anticoagulants for use in this assay) and cell culture supernatants. This assay employs an antibody specific for human Munc18-1 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "79 - 105 %", "average": "= 88.12" }, { "sample": "Plasma", "range": "74 - 97 %", "average": "= 85.26" }, { "sample": "Cell culture media", "range": "89 - 113 %", "average": "= 101.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314357-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Human Munc18-1 Antibody-coated ELISA Plate", "number":"AB314357-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314357-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314357-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Munc18-1 Protein Standard", "number":"AB314357-CMP11", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB314357-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"800X HRP-Streptavidin", "number":"AB314357-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314357-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314357-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Munc18-1 Detection Antibody", "number":"AB314357-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Munc18-1 also known as Syntaxin-binding protein 1 is an important protein involved in the regulation of neurotransmitter release at synapses. It has a molecular mass of approximately 67 kDa. This protein is highly expressed in the brain where it plays a significant role in synaptic vesicle exocytosis. Munc18-1 interacts with components of the SNARE complex facilitating the docking and fusion of synaptic vesicles with the presynaptic membrane.
Biological function summary

This protein is important for the proper release of neurotransmitters into the synaptic cleft. Munc18-1 functions as a part of the neuronal exocytosis complex where it regulates the interaction with syntaxin-1 a core SNARE protein. This protein-protein interaction stabilizes an intermediate state necessary for vesicle priming and subsequent fusion ensuring efficient synaptic transmission. By controlling vesicle movement Munc18-1 influences communication between neurons.

Pathways

Munc18-1 integrates into the neurotransmitter release pathway which is central to synaptic function. It closely associates with the SNARE complex involving proteins such as SNAP-25 and synaptobrevin. Another important pathway is the synaptic vesicle cycle where Munc18-1 plays a role in vesicle docking and priming stages. Through these interactions it ensures that synaptic vesicles are ready and able to release their contents upon stimulation.

Alterations in Munc18-1 expression or function connect to neurological conditions like epilepsy and intellectual disability. In epilepsy dysregulation of neurotransmitter release due to modified Munc18-1 function disrupts synaptic communication leading to seizure activity. In the context of these disorders Munc18-1 interacts functionally with proteins such as VAMP2 contributing to the pathological state when disrupted. These interactions highlight the importance of Munc18-1 in maintaining normal neurological function.
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Product protocols

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Target data

Participates in the regulation of synaptic vesicle docking and fusion through interaction with GTP-binding proteins (By similarity). Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1 : 1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. Involved in the release of neurotransmitters from neurons through interacting with SNARE complex component STX1A and mediating the assembly of the SNARE complex at synaptic membranes (By similarity). May play a role in determining the specificity of intracellular fusion reactions.
See full target information STXBP1
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websiteProtocolBooklet
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Product promise

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