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AB314367

Human MX1 ELISA Kit

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Human MX1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MX1 with a sensitivity of 14.15 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Interferon-induced GTP-binding protein Mx1, Interferon-induced protein p78, Interferon-regulated resistance GTP-binding protein MxA, Myxoma resistance protein 1, Myxovirus resistance protein 1, IFI-78K, MX1

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Sandwich ELISA - Human MX1 ELISA Kit (AB314367)
  • sELISA

Supplier Data

Sandwich ELISA - Human MX1 ELISA Kit (AB314367)

Interpolated concentration of native MX1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 25 µg/mL Daudi + IFN alpha Extract, and 50 µg/mL tonsil tissue extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human MX1 ELISA Kit (AB314367)
  • sELISA

Supplier Data

Sandwich ELISA - Human MX1 ELISA Kit (AB314367)

Example of human MX1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human MX1 ELISA Kit (AB314367)
  • sELISA

Supplier Data

Sandwich ELISA - Human MX1 ELISA Kit (AB314367)

Daudi cells were treated overnight with 20 µg/mL human IFN-alpha then followed by protein extraction. Mock control Daudi cells accompanied cell treatment. Concentrations of human MX1 were measured in duplicate and interpolated against the standard curve. Results show the mean human MX1 was not detected (ND) in the mock samples compared to 2883 pg/mL in IFN-alpha treated samples.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 14.15 pg/mL

Range

70.313 - 4500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human MX1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MX1 protein in Cell Lysate, Tissue extracts. Quantitate Human MX1 with 14.15 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
        -High sensitivity, specificity and reproducibility from superior antibodies
        -Fully validated in biological samples
        -96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "84 - 99 %", "average": "= 93" }, { "sample": "Tissue Extracts", "range": "98 - 113 %", "average": "= 105" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Interferon-induced dynamin-like GTPase with antiviral activity against a wide range of RNA viruses and some DNA viruses. Its target viruses include negative-stranded RNA viruses and HBV through binding and inactivation of their ribonucleocapsid. May also antagonize reoviridae and asfarviridae replication. Inhibits thogoto virus (THOV) replication by preventing the nuclear import of viral nucleocapsids. Inhibits La Crosse virus (LACV) replication by sequestering viral nucleoprotein in perinuclear complexes, preventing genome amplification, budding, and egress. Inhibits influenza A virus (IAV) replication by decreasing or delaying NP synthesis and by blocking endocytic traffic of incoming virus particles. Enhances ER stress-mediated cell death after influenza virus infection. May regulate the calcium channel activity of TRPCs.
See full target information MX1
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB237299

Alexa Fluor® 647 Anti-MX1 antibody [EPR19967]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-MX1 antibody [EPR19967] (AB237299)

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Proteins & Peptides

AB187486

Recombinant Human MX1 protein

SDS-PAGE - Recombinant Human MX1 protein (AB187486)

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Product promise

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For full details, please see our Terms & Conditions

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