JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB171341

Human MyD88 ELISA Kit

Be the first to review this product! Submit a review

|

(8 Publications)

Human MyD88 ELISA Kit is a Sandwich ELISA for the measurement of Human MyD88 in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Myeloid differentiation primary response protein MyD88, MYD88

1 Images
Sandwich ELISA - Human MyD88 ELISA Kit (AB171341)
  • sELISA

Supplier Data

Sandwich ELISA - Human MyD88 ELISA Kit (AB171341)

Representative standard curve using ab171341

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay Platform

Microplate

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB176663

NF kappaB p65 (pS536 + Total) ELISA Kit

SimpleStep

ELISA - NF kappaB p65 (pS536 + Total) ELISA Kit (AB176663)

  • 0
  • 0
Assay Kits

AB274401

Human NLRP3 ELISA Kit

Recombinant|SimpleStep

ELISA - Human NLRP3 ELISA Kit (AB274401)

  • 3
  • 1
Assay Kits

AB113851

DCFDA / H2DCFDA - Cellular ROS Assay Kit

Cellular Activity - DCFDA / H2DCFDA - Cellular ROS Assay Kit (AB113851)

  • 5
  • 11
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's Human MyD88 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human MyD88 in cell culture supernatants, cell lysates, serum and plasma.

A MyD88 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for MyD88 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human MyD88 amount of sample captured in plate.

style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab171341-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB171341-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-Human MyD88 antibody", "number":"AB171341-CMP09", "productcode":"" }, { "size":"1 x 96 Test", "name":"Anti-Human MyD88 Antibody Microplate (12 x 8 wells)", "number":"AB171341-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB171341-CMP06", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB171341-CMP10", "productcode":"" }, { "size":"1 x 4 Unit", "name":"Plate Seal", "number":"AB171341-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB171341-CMP08", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant Human MyD88 standard", "number":"AB171341-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB171341-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB171341-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB171341-CMP05", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MyD88 standing for myeloid differentiation primary response 88 is a cytoplasmic adaptor protein with a molecular weight of approximately 33 kDa. This protein is expressed widely in immune cells including monocytes macrophages and dendritic cells. MyD88 serves a major role in the signaling pathways for the innate immune system. It acts as a linker transmitting signals from toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs) to downstream signaling molecules ultimately activating transcription factors.
Biological function summary

MyD88 plays a significant role in mediating immune responses by forming part of a complex that includes IRAK kinases and TRAF6. When TLRs or IL-1Rs activate MyD88 this adaptor protein recruits IRAK4 which then phosphorylates IRAK1 or IRAK2. This cascade promotes the activation of NF-κB and MAPK pathways leading to the production of inflammatory cytokines. The MyD88-dependent pathway is integral to innate immunity influencing how the body responds to pathogen infection and inflammation.

Pathways

MyD88 integrates into both the TLR signaling and IL-1R signaling pathways. Key related proteins in these pathways include interleukin-1 receptor-associated kinase (IRAK) and tumor necrosis factor receptor-associated factor 6 (TRAF6). MyD88 initiates the recruitment and activation of IRAK1 and IRAK4 following receptor engagement leading to subsequent activation of downstream signals. As part of these pathways MyD88 mediates cellular responses important for immune system signaling and inflammatory response regulation.

MyD88 involvement is notable in the context of oncological and autoimmune diseases. Mutations or dysregulation of MyD88 are implicated in conditions like lymphoma and rheumatoid arthritis. In lymphoma the mutation usually results in constant activation of NF-κB leading to unchecked cell growth. As for rheumatoid arthritis an overactive immune response due to MyD88 can cause additional inflammation affecting joint tissues. In these conditions MyD88 interaction with IRAK4 is significant given their mutual role in immune and inflammatory pathways.
style
left-column

Product protocols

style
left-column

Target data

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response (PubMed : 15361868, PubMed : 18292575, PubMed : 33718825, PubMed : 37971847). Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 15361868, PubMed : 19506249, PubMed : 24316379, PubMed : 40638072). Increases IL-8 transcription (PubMed : 9013863). Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Upon TLR8 activation by GU-rich single-stranded RNA (GU-rich RNA) derived from viruses such as SARS-CoV-2, SARS-CoV and HIV-1, induces IL1B release through NLRP3 inflammasome activation (PubMed : 33718825). MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine (By similarity).
See full target information MYD88
style
left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutics 16: PubMed39204330

2024

Hyaluronic Acid-Based Nanoparticles Loaded with Rutin as Vasculo-Protective Tools against Anthracycline-Induced Endothelial Damages.

Applications

Unspecified application

Species

Unspecified reactive species

Carla Serri,Vincenzo Quagliariello,Iriczalli Cruz-Maya,Vincenzo Guarino,Nicola Maurea,Paolo Giunchedi,Giovanna Rassu,Elisabetta Gavini

Frontiers in cardiovascular medicine 11:1289663 PubMed38818214

2024

Sodium-glucose cotransporter 2 inhibitor dapagliflozin prevents ejection fraction reduction, reduces myocardial and renal NF-κB expression and systemic pro-inflammatory biomarkers in models of short-term doxorubicin cardiotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

V Quagliariello,M L Canale,I Bisceglia,M Iovine,A Paccone,C Maurea,M Scherillo,A Merola,V Giordano,G Palma,A Luciano,F Bruzzese,F Zito Marino,M Montella,R Franco,M Berretta,D Gabrielli,G Gallucci,N Maurea

Cancers 16: PubMed38672567

2024

The sGCa Vericiguat Exhibit Cardioprotective and Anti-Sarcopenic Effects through NLRP-3 Pathways: Potential Benefits for Anthracycline-Treated Cancer Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Vincenzo Quagliariello,Massimiliano Berretta,Irma Bisceglia,Ilaria Giacobbe,Martina Iovine,Vienna Giordano,Raffaele Arianna,Matteo Barbato,Francesca Izzo,Carlo Maurea,Maria Laura Canale,Andrea Paccone,Alessandro Inno,Marino Scherillo,Domenico Gabrielli,Nicola Maurea

Phytotherapy research : PTR 38:82-97 PubMed37807970

2023

Ursolic acid alleviates chronic prostatitis via regulating NLRP3 inflammasome-mediated Caspase-1/GSDMD pyroptosis pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng-Jing Liu,Bo-da Guo,Qing-He Gao,Ying-Jun Deng,Bin Yan,Yin Zeng,Ming Zhao,Kai Ren,Fu Wang,Jun Guo

International journal of molecular sciences 23: PubMed35163130

2022

Oleacein Attenuates Lipopolysaccharide-Induced Inflammation in THP-1-Derived Macrophages by the Inhibition of TLR4/MyD88/NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Santa Cirmi,Alessandro Maugeri,Caterina Russo,Laura Musumeci,Michele Navarra,Giovanni Enrico Lombardo

Frontiers in oncology 11:680758 PubMed34178667

2021

Polydatin Reduces Cardiotoxicity and Enhances the Anticancer Effects of Sunitinib by Decreasing Pro-Oxidative Stress, Pro-Inflammatory Cytokines, and NLRP3 Inflammasome Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Vincenzo Quagliariello,Massimiliano Berretta,Simona Buccolo,Martina Iovine,Andrea Paccone,Ernesta Cavalcanti,Rosaria Taibi,Monica Montopoli,Gerardo Botti,Nicola Maurea

Molecular medicine reports 23: PubMed33655339

2021

Involvement of TLR2/4‑MyD88‑NF‑κB signaling pathway in the pathogenesis of intracranial aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Xuezhi Zhang,Yilv Wan,Jiugeng Feng,Meihua Li,Zhiqun Jiang

International journal of molecular sciences 21: PubMed33096896

2020

NLRP3 as Putative Marker of Ipilimumab-Induced Cardiotoxicity in the Presence of Hyperglycemia in Estrogen-Responsive and Triple-Negative Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Vincenzo Quagliariello,Michelino De Laurentiis,Stefania Cocco,Giuseppina Rea,Annamaria Bonelli,Antonietta Caronna,Maria Cristina Lombari,Gabriele Conforti,Massimiliano Berretta,Gerardo Botti,Nicola Maurea
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com