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Human MyD88 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human MyD88 in Human in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma samples.

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Sandwich ELISA - Human MyD88 ELISA Kit (AB171341), expandable thumbnail

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Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

156 - 10000 pg/mL

Sensitivity

< 10 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response (PubMed:15361868, PubMed:18292575, PubMed:33718825, PubMed:37971847). Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:15361868, PubMed:19506249, PubMed:24316379). Increases IL-8 transcription (PubMed:9013863). Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Upon TLR8 activation by GU-rich single-stranded RNA (GU-rich RNA) derived from viruses such as SARS-CoV-2, SARS-CoV and HIV-1, induces IL1B release through NLRP3 inflammasome activation (PubMed:33718825). MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine (By similarity).

Alternative names

What's included?

1 x 96 Tests
Components
ABC Diluent Buffer
1 x 12 mL
Anti-Human MyD88 Antibody Microplate (12 x 8 wells)
1 x 96 Test
Antibody Diluent Buffer
1 x 12 mL
Avidin-Biotin-Peroxidase Complex (ABC)
1 x 100 µL
Biotinylated anti-Human MyD88 antibody
1 x 100 µL
Lyophilized recombinant Human MyD88 standard
2 x 10 ng
Plate Seal
1 x 4 Unit
Sample Diluent Buffer
1 x 30 mL
TMB Color Developing Agent
1 x 10 mL
TMB Stop Solution
1 x 10 mL
Wash Buffer (25X)
1 x 20 mL

Recommended products

Human MyD88 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human MyD88 in Human in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma samples.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

156 - 10000 pg/mL

Assay Platform

Microplate

Sensitivity

< 10 pg/mL

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Abcam's Human MyD88 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human MyD88 in cell culture supernatants.

A MyD88 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for MyD88 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human MyD88 amount of sample captured in plate.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MyD88 standing for myeloid differentiation primary response 88 is a cytoplasmic adaptor protein with a molecular weight of approximately 33 kDa. This protein is expressed widely in immune cells including monocytes macrophages and dendritic cells. MyD88 serves a major role in the signaling pathways for the innate immune system. It acts as a linker transmitting signals from toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs) to downstream signaling molecules ultimately activating transcription factors.

Biological function summary

MyD88 plays a significant role in mediating immune responses by forming part of a complex that includes IRAK kinases and TRAF6. When TLRs or IL-1Rs activate MyD88 this adaptor protein recruits IRAK4 which then phosphorylates IRAK1 or IRAK2. This cascade promotes the activation of NF-κB and MAPK pathways leading to the production of inflammatory cytokines. The MyD88-dependent pathway is integral to innate immunity influencing how the body responds to pathogen infection and inflammation.

Pathways

MyD88 integrates into both the TLR signaling and IL-1R signaling pathways. Key related proteins in these pathways include interleukin-1 receptor-associated kinase (IRAK) and tumor necrosis factor receptor-associated factor 6 (TRAF6). MyD88 initiates the recruitment and activation of IRAK1 and IRAK4 following receptor engagement leading to subsequent activation of downstream signals. As part of these pathways MyD88 mediates cellular responses important for immune system signaling and inflammatory response regulation.

Associated diseases and disorders

MyD88 involvement is notable in the context of oncological and autoimmune diseases. Mutations or dysregulation of MyD88 are implicated in conditions like lymphoma and rheumatoid arthritis. In lymphoma the mutation usually results in constant activation of NF-κB leading to unchecked cell growth. As for rheumatoid arthritis an overactive immune response due to MyD88 can cause additional inflammation affecting joint tissues. In these conditions MyD88 interaction with IRAK4 is significant given their mutual role in immune and inflammatory pathways.

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1 product image

  • Sandwich ELISA - Human MyD88 ELISA Kit (ab171341), expandable thumbnail

    Sandwich ELISA - Human MyD88 ELISA Kit (ab171341)

    Representative standard curve using ab171341

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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