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AB284014

Human Myeloperoxidase Autoantibody ELISA Kit

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Human Myeloperoxidase Autoantibody ELISA Kit is a Sandwich ELISA for the measurement of Human Myeloperoxidase Autoantibody in Human in Biofluids samples.

View Alternative Names

Myeloperoxidase, MPO

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Sandwich ELISA - Human Myeloperoxidase Autoantibody ELISA Kit (AB284014)
  • sELISA

Supplier Data

Sandwich ELISA - Human Myeloperoxidase Autoantibody ELISA Kit (AB284014)

Example of Human Myeloperoxidase Autoantibody standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.56 AU/mL

Range

1.563 - 25 AU/mL

Assay time

4h

Assay Platform

Microplate (12 x 8 well strips)

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human Myeloperoxidase Autoantibody ELISA (ab284014) Kit is designed for the quantitative determination of autoimmune response (IgG) to a target antigen (MPO). The kit detects autoantibodies in human plasma and serum samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures autoantibodies (anti-MPO IgG) in less than 4 hours.

A MPO antigen has been pre-coated onto a 96-well microplate with removable strips. An autoantibody specific for MPO in standards and samples is sandwiched by the immobilized antigen and a biotinylated polyclonal antibody specific for human IgG, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab284014-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Myeloperoxidase Autoantibody Standard", "number":"AB284014-CMP06", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB284014-CMP04", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB284014-CMP09", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB284014-CMP08", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB284014-CMP03", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Myeloperoxidase Autoantibody Antibody", "number":"AB284014-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Myeloperoxidase Autoantibody Microplate", "number":"AB284014-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Diluent M Concentrate", "number":"AB284014-CMP01", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB284014-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.
Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.
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Product protocols

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Target data

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).
See full target information MPO
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Product promise

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