AB272101

Human Myeloperoxidase ELISA Kit

Recombinant
SimpleStep
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(7 Publications)

Human Myeloperoxidase ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Myeloperoxidase with a sensitivity of 33.37 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Myeloperoxidase, MPO

7 Images

ELISA - Human Myeloperoxidase ELISA Kit (AB272101)

ELISA

Supplier Data

ELISA - Human Myeloperoxidase ELISA Kit (AB272101)

sELISA

Supplier Data

Sandwich ELISA - Human Myeloperoxidase ELISA Kit (AB272101)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MPO concentration was determined to be 20.92 ng/mL with a range of 11.65 – 38.79 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Myeloperoxidase ELISA Kit (AB272101)

Human MPO competitor curve comparison.

Standard Curve comparison between Human MPO SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human Myeloperoxidase ELISA Kit (AB272101)

Example of human MPO standard curve in Sample Diluent NS.

The MPO standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Myeloperoxidase ELISA Kit (AB272101)

Human MPO standard curve comparison.

Standard Curve comparison between the original Human MPO SimpleStep ELISA kit (ab195212) and current Human MPO SimpleStep ELISA kit (ab272101).

sELISA

Supplier Data

Sandwich ELISA - Human Myeloperoxidase ELISA Kit (AB272101)

Interpolated concentrations of native MPO in human serum, plasma and cell culture supernatant samples.

The concentrations of MPO were measured in duplicates, interpolated from the MPO standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (citrate) 25%, plasma (EDTA) 25%, and HL-60 supernatant 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MPO concentration was determined to be 7.38 ng/mL in serum, 20.34 ng/mL in plasma (citrate), 13.06 ng/ml plasma (EDTA), and 1,035.38 ng/ml in HL-60 supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human Myeloperoxidase ELISA Kit (AB272101)

Interpolated concentrations of native MPO in human HL-60 cell culture extract based on a 12.5 μg/mL extract load.

The concentrations of MPO were measured in duplicate and interpolated from the MPO standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MPO concentration was determined to be 3.69 ng/ml in HL-60 cell culture extract.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Cell culture extracts, Cell culture media, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 33.37 pg/mL

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Myeloperoxidase ELISA Kit ab272101 is a rapid single-wash 90-min Sandwich ELISA to measure Human Myeloperoxidase in cell culture extracts, cell culture media, cell culture supernatant, citrate plasma, EDTA plasma, saliva, serum, urine. This SimpleStep sensitivity is 33.37 pg/mL.

How the assay works

Human Myeloperoxidase SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Myeloperoxidase ELISA Kit ab272101 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Myeloperoxidase antibody [EPR22162-183] - BSA and Azide free (Capture) ab267426
- Anti-Myeloperoxidase antibody [EPR22162-121] - BSA and Azide free (Detector) ab267426

Human Myeloperoxidase (MPO) is a hemoprotein expressed in the neutrophils (polymorphonuclear leukocytes) and is secreted during activation. Myeloperoxidase catalyzes the oxidation of chloride ions to hypochlorus acid, which is a potent antimicrobial agent. Myeloperoxidase pro-oxidative and pro-inflammatory properties cause an increase in MPO in multiple diseases including heart disease, myocardial infraction, and multiple sclerosis. Myeloperoxidase is a clinical marker of myocardial infraction and cardiovascular disease.

The standard protein in this product is purified from whole blood shown to be non-reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "94 - 104 %", "average": "= 99" }, { "sample": "Serum", "range": "88 - 94 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "86 - 92 %", "average": "= 89" }, { "sample": "Saliva", "range": "94 - 118 %", "average": "= 103" }, { "sample": "Urine", "range": "85 - 95 %", "average": "= 89" }, { "sample": "Cell culture extracts", "range": "99 - 108 %", "average": "= 104" }, { "sample": "Cell culture media", "range": "86 - 98 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "86 - 91 %", "average": "= 88" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272101-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB272101-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB272101-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB272101-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Myeloperoxidase (MPO) Lyophilized Purified Protein", "number":"AB272101-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB272101-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Myeloperoxidase (MPO) Capture Antibody", "number":"AB272101-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB272101-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB272101-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (12 x 8 wells)", "number":"AB272101-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB272101-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Myeloperoxidase (MPO) Detector Antibody", "number":"AB272101-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

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Product protocols

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Target data

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).

See full target information MPO

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:22557 PubMed40595199

2025

A pilot clinical trial to explore the effects of UV exposure on vitamin D synthesis and inflammatory responses in vitamin D-Deficient adults.

Applications

Unspecified application

Species

Unspecified reactive species

Chaodeng Mo,Johny Bajgai,Md Habibur Rahman,Soonchul Choe,Sofian Abdul-Nasir,Hui Ma,Wenjing He,Thu Thao Pham,Haiyang Zhang,Goh Seong Hoon,Kim Bomi,Cheol-Su Kim,Kyu-Jae Lee,Insik Shin

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2411524 PubMed40013981

2025

NETs-CD44-IL-17A Feedback Loop Drives Th17-Mediated Inflammation in Behçet's Uveitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Wu,Kang Ning,Zhaohao Huang,Binyao Chen,Junjie Chen,Yingying Wen,Jian Bu,Han Hong,Qiaorong Chen,Zhuoqi Zhang,Renbing Jia,Wenru Su

Cancers 16: PubMed38398138

2024

Characterizing the Inflammatory Profile of Neutrophil-Rich Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fatma Al Qutami,Walaa AlHalabi,Aswathy Vijayakumar,Surendra Singh Rawat,Abubakr H Mossa,Manju Nidagodu Jayakumar,Baila Samreen,Mahmood Y Hachim

Life (Basel, Switzerland) 13: PubMed38004280

2023

Enhanced Microvascular Adaptation to Acute Physical Stress and Reduced Oxidative Stress in Male Athletes Who Consumed Chicken Eggs Enriched with n-3 Polyunsaturated Fatty Acids and Antioxidants-Randomized Clinical Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Luka Kolar,Petar Šušnjara,Marko Stupin,Ana Stupin,Ivana Jukić,Zrinka Mihaljević,Nikolina Kolobarić,Iva Bebek,Diana Nejašmić,Marija Lovrić,Ines Drenjančević

Redox biology 67:102906 PubMed37812880

2023

Neutrophil extracellular traps drive intestinal microvascular endothelial ferroptosis by impairing Fundc1-dependent mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Chengnan Chu,Xinyu Wang,Chao Yang,Fang Chen,Lin Shi,Weiqi Xu,Kai Wang,Baochen Liu,Chenyang Wang,Dongping Sun,Weiwei Ding

Cell proliferation 57:e13538 PubMed37691112

2023

Neutrophil extracellular traps aggravate intestinal epithelial necroptosis in ischaemia-reperfusion by regulating TLR4/RIPK3/FUNDC1-required mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Chengnan Chu,Xinyu Wang,Fang Chen,Chao Yang,Lin Shi,Weiqi Xu,Kai Wang,Baochen Liu,Chenyang Wang,Dongping Sun,Jieshou Li,Weiwei Ding

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e21552 PubMed33826788

2021

Microbial-derived indoles inhibit neutrophil myeloperoxidase to diminish bystander tissue damage.

Applications

Unspecified application

Species

Unspecified reactive species

Erica E Alexeev,Alexander S Dowdell,Morkos A Henen,Jordi M Lanis,J Scott Lee,Ian M Cartwright,Rachel E M Schaefer,Alfredo Ornelas,Joseph C Onyiah,Beat Vögeli,Sean P Colgan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Myeloperoxidase ELISA Kit

ELISA - Human Myeloperoxidase ELISA Kit (AB272101)

Sandwich ELISA - Human Myeloperoxidase ELISA Kit (AB272101)

Sandwich ELISA - Human Myeloperoxidase ELISA Kit (AB272101)

Sandwich ELISA - Human Myeloperoxidase ELISA Kit (AB272101)

Sandwich ELISA - Human Myeloperoxidase ELISA Kit (AB272101)

Sandwich ELISA - Human Myeloperoxidase ELISA Kit (AB272101)

Sandwich ELISA - Human Myeloperoxidase ELISA Kit (AB272101)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Complementary Products

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Primary Antibodies

Assay Kits

Secondary Antibodies

Product promise