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AB119605

Human Myeloperoxidase ELISA Kit (MPO)

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(39 Publications)

Human Myeloperoxidase ELISA Kit (MPO) is a sandwich ELISA designed to quantify Human Myeloperoxidase (MPO) with a sensitivity of 10 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 312 - 20000 pg/mL
- Cited in over 30 publications

View Alternative Names

Myeloperoxidase, MPO

2 Images
Sandwich ELISA - Human Myeloperoxidase ELISA Kit (MPO) (AB119605)
  • sELISA

Supplier Data

Sandwich ELISA - Human Myeloperoxidase ELISA Kit (MPO) (AB119605)

Representative Standard Curve using ab119605.

Enzyme Immunoassay - Human Myeloperoxidase ELISA Kit (MPO) (AB119605)
  • EIA

CiteAb

Enzyme Immunoassay - Human Myeloperoxidase ELISA Kit (MPO) (AB119605)

Myeloperoxidase Enzyme-linked immunosorbent assay using Human Myeloperoxidase ELISA Kit (MPO), ab119605. Publication image from Koupenova, M. et al., 2019, Nat Commun, PubMed : 30992428.

Platelet-TLR7mediates release of MPO from neutrophils. Isolated human platelets and neutrophils were incubated together or by themselves for 30 min, at 37 °C and constant rotation, in the presence of TLR agonists [TLR7 (Loxo)—1 mM; TLR2 (Pam)—10 μg/mL] or thrombin (IIa)—0.05 U/mL. Myeloperoxidase (MPO) release from neutrophils was measured in the a absence (p = 0.7206, F = 0.4493, df = 3) and b presence of platelets by ELISA (p = 0.0002, F = 10.4, df = 3). The graphs represent the average fold change for each individual of n = 6 (3F; 3M) ± SD. Source data are provided as a Source Data file. Significance was measured by ANOVA followed by Bonferroni follow-up test; in all cases star symbol (*) indicates p < 0.05

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Saliva, Serum, Tissue Homogenate, Urine

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

312 - 20000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Myeloperoxidase ELISA Kit (MPO) ab119605 is a sandwich ELISA to measure Human Myeloperoxidase (MPO) in serum, EDTA plasma, heparin plasma, cell culture supernatant, saliva, urine, cell lysate, tissue homogenate with a sensitivity of 10 pg/ml.

How the assay works

A Myeloperoxidase specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Myeloperoxidase is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Myeloperoxidase amount of sample captured in plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Myeloperoxidase ELISA Kit (MPO) ab119605 protocol summary

1. Add standard or sample to each well used. Incubate 37C
2. Add prepared biotin antibody to each well. Incubate at 37C
3. Add prepared Avidin-BiotinPeroxidase Complex (ABC). Incubate at 37C
4. Add TMB to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450 nm

Get results in 90 minutes with our SimpleStep ELISA® kit

Human Myeloperoxidase ELISA Kit (ab195212)
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.
Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.
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Product protocols

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Target data

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).
See full target information MPO
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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Kidney research and clinical practice : PubMed40528475

2025

A comparative study of oxidative stress, vascular calcification, and volume status in non-dippers and dippers receiving maintenance hemodialysis.

Applications

Unspecified application

Species

Unspecified reactive species

Hyangkyoung Kim,Jungho Shin,Jin Ho Hwang,Hye Ryoun Kim,Cheul Hong Min,Hyung-Eun Son,Semin Cho,Jeong Min Cho,Soie Kwon,Su Hyun Kim

NPJ Parkinson's disease 11:94 PubMed40287421

2025

Association between serum myeloperoxidase enzyme activity and Parkinson's disease status.

Applications

Unspecified application

Species

Unspecified reactive species

Emilio Fernández Espejo,María-Del-Mar Guerra,Silvia Castellano

Gastroenterology research and practice 2025:4950214 PubMed40046958

2025

Neutrophil Extracellular Traps Promote Pancreatic Cancer Progression via the STING Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mengdi Qu,Chenyu Zhu,Caihong Sun,Shuainan Zhu,Hao Zhang,Changhong Miao,Di Zhou

Nutrients 16: PubMed39599716

2024

Relationship Between Serum Levels of Oxidized Lipoproteins, Circulating Levels of Myeloperoxidase and Paraoxonase 1, and Diet in Young Subjects with Insulin Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Yaquelin Marchán-Figueroa,Brenda Tepec-Casarrubias,Ulises de la Cruz-Mosso,Constanza Cecilia Astudillo-López,Inés Matia-García,Lorenzo Salgado-Goytia,Mónica Espinoza-Rojo,Natividad Castro-Alarcón,Eugenia Flores-Alfaro,Isela Parra-Rojas

Frontiers in molecular biosciences 11:1252058 PubMed38584705

2024

Serum proteomic profiling of precancerous gastric lesions and early gastric cancer reveals signatures associated with systemic inflammatory response and metaplastic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yueqing Gong,Yaxin Lou,Xiurui Han,Keyan Chen,Yang Zhao,Hejun Zhang,Jing Zhang,Ying Xiong,Weiwei Fu,Shigang Ding

Cell communication and signaling : CCS 22:97 PubMed38308264

2024

Ferritin-mediated neutrophil extracellular traps formation and cytokine storm via macrophage scavenger receptor in sepsis-associated lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhang,Dan Wu,Yanghanzhao Wang,Yuxin Shi,Yuwen Shao,Fu Zeng,Charles B Spencer,Lilibeth Ortoga,Dehua Wu,Changhong Miao

Nature communications 14:5894 PubMed37736772

2023

CREB1-driven CXCR4 neutrophils promote skin inflammation in mouse models and human patients.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaoling Chen,Yaxing Bai,Ke Xue,Zhiguo Li,Zhenlai Zhu,Qingyang Li,Chen Yu,Bing Li,Shengxian Shen,Pei Qiao,Caixia Li,Yixin Luo,Hongjiang Qiao,Erle Dang,Wen Yin,Johann E Gudjonsson,Gang Wang,Shuai Shao

Experimental & molecular medicine 55:939-951 PubMed37121969

2023

Mesenchymal stem cell-derived extracellular vesicles protect against abdominal aortic aneurysm formation by inhibiting NET-induced ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Chen,Yuting Liu,Zheyu Wang,Leiyang Zhang,Yi Xu,Yinan Li,Lan Zhang,Guiming Wang,Shuofei Yang,Guanhua Xue

Scientific reports 13:4903 PubMed36966172

2023

The abundances of LTF and SOD2 in amniotic fluid are potential biomarkers of gestational age and preterm birth.

Applications

Unspecified application

Species

Unspecified reactive species

Te-Yao Hsu,Hsin-Hsin Cheng,Kuo-Chung Lan,Hsuan-Ning Hung,Yun-Ju Lai,Chih-Chang Tsai,Wen-Lang Fan,Sung-Chou Li

Journal of proteomics 278:104865 PubMed36870676

2023

Biochemical network analysis of protein-protein interactions to follow-up T1 bladder cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Luís B Carvalho,José Luis Capelo Martínez,Carlos Lodeiro,Rafael Bento,Rajiv Dhir,Jeremiah J Morrissey,Luis Campos Pinheiro,Mariana Medeiros,Hugo M Santos
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