Human Myosin light chain 3 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Myosin light chain 3 in Cell culture extracts, Tissue Extracts samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 125 - 8000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 89 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Regulatory light chain of myosin. Does not bind calcium.
Alternative names
Myosin light chain 3, Cardiac myosin light chain 1, Ventricular myosin alkali light chain, Ventricular myosin light chain 1, Ventricular/slow twitch myosin alkali light chain, CMLC1, MLC1SB, VLCl, MLC-lV/sb, MYL3
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Human Myosin light chain 3 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Myosin light chain 3 in Cell culture extracts, Tissue Extracts samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 125 - 8000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 89 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 1.9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Tissue Extracts | Average % = 95 | Range 92 - 100 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Myosin light chain 3 ELISA Kit (ab246538) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Myosin light chain 3 protein in tissue extracts and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Myosin light chain 3 with 89 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myosin light chain 3 (MLC3) also known as myosin regulatory light chain 3 is a part of the myosin protein complex. It plays a major role in muscle contraction by regulating the ATPase activity of myosin heads. MLC3 is encoded by the MYL3 gene and has a molecular mass of approximately 18kDa. It is expressed mainly in cardiac and skeletal muscle tissues. By interacting with myosin heavy chains MLC3 contributes to the force-generating capacity of muscles.
- Biological function summary
MLC3 functions as an essential component of the myosin light chain complex. This complex regulates contractile activity in muscle cells by modulating the interaction between actin and myosin chains. MLC3 activates the mechanical movement of myosin chains impacting cardiac and skeletal muscle contraction. When activated it affects the speed and strength of muscle contraction and it also has a role in muscle differentiation and development.
- Pathways
MLC3 is an integral part of the muscle contraction pathway. It interacts with proteins such as tropomyosin and troponin which are important for the regulation of muscle contraction. Additionally MLC3 participates in the calcium signaling pathway which is pivotal for contraction processes in muscles. This interaction helps to control the cytosolic calcium concentration a critical factor in muscle contraction dynamics.
- Associated diseases and disorders
MLC3 has connections to cardiomyopathy and hypertrophic conditions. Mutations or dysregulation of MYL3 can lead to cardiac hypertrophy a disease where the heart muscle thickens abnormally. MLC3's interaction with troponin and other myosin chains can exacerbate cardiac conditions when these pathways are disrupted. Studies have linked deficiencies in MLC3 to improper muscle development which may underline some muscle myopathies.
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2 product images
Sandwich ELISA - Human Myosin light chain 3 ELISA Kit (ab246538)
Example of human Myosin Light Chain 3 standard curve in 1X Cell Extraction Buffer PTR.
The Myosin Light Chain 3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Myosin light chain 3 ELISA Kit (ab246538)
Interpolated concentrations of native Myosin Light Chain 3 in human heart and skeletal muscle extract samples based on a 72 μg/mL and 20 µg/mL extract load respectively.
The concentrations of Myosin Light Chain 3 were measured in duplicate and interpolated from the Myosin Light Chain 3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myosin Light Chain 3 concentration was determined to be 1538 pg/mL in heart extract and 2893 pg/mL in skeletal muscle extract.
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