Human Myostatin ELISA kit

Human Myostatin ELISA Kit is designed for the quantitative determination of Myostatin in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human Myostatin coated on a 96-well plate. Standards and samples are pipetted into the wells and Myostatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Myostatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Myostatin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: Human ACE, Activin RIB (ALK-4), ADAM23, Artemin, Cardiotrophin-1 (CT-1), Cathepsin V, FABP1 (LFABP), FGF-20, HAI-1, IL-27 R alpha (WSX-1), Insulin R (CD220), Kallikrein 7, LIF R alpha, Lipocalin-1, LTbR (Lymphotoxin beta R), Mesothelin, MFRP, Neuropilin-2, Neurturin, Nidogen-2, Olfactomedin-2 (Noelin-2), p53, PDECGF (Thymidine Phosphorylase), PDGF-CC, Progranulin, Ret, ROBO4, Semaphorin 6B, Serpin F1 (PEDF), SREC-I (SCARF1), SREC-II (SCARF2), TLR1, TLR3, TPP1 (Tripeptidyl-peptidase I), TREM-2, TrkC, TROY (TNFRSF19), Uromodulin, XIAP.