Human N-368 Tau ELISA Kit- Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human N-368 Tau - Extracellular in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Cerebral Spinal Fluid samples.
Colorimetric
Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Cerebral Spinal Fluid
Sandwich (quantitative)
Human
46.875 - 3000 pg/mL
1h 30m
= 11.829 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Human N-368 Tau ELISA Kit- Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human N-368 Tau - Extracellular in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Cerebral Spinal Fluid samples.
Colorimetric
Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Cerebral Spinal Fluid
Sandwich (quantitative)
Human
46.875 - 3000 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 11.829 pg/mL
84%
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 4.3 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 5 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 84 | Range 79 - 86 % |
Sample type Citrate plasma | Average % = 81 | Range 79 - 84 % |
Sample type EDTA Plasma | Average % = 80 | Range 78 - 82 % |
Sample type Heparin Plasma | Average % = 115 | Range 107 - 121 % |
Sample type Cell culture media | Average % = 86 | Range 83 - 89 % |
Sample type Cerebral Spinal Fluid | Average % = 94 | Range 92 - 96 % |
Blue Ice
+4°C
+4°C
+4°C
Human N-368 Tau SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of N-368 Tau protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media, Cerebral Spinal Fluid. Quantitate Human N-368 Tau with 11.829 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
This supplementary information is collated from multiple sources and compiled automatically.
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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Example of human N-368 Tau standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Recombinant N-368 Tau was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are as follows: 25% human serum, 25% plasma (citrate), 25% plasma (EDTA), 25% plasma (heparin), 50% cell culture media, and 50% CSF. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean N-368 Tau concentration of detected samples was determined to be 76.61 pg/mL with a range of undetected – 92.97 pg/mL.
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