JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB315060

Human N-368 Tau ELISA Kit- Extracellular

Be the first to review this product! Submit a review

|

(0 Publication)

Human N-368 Tau ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human N-368 Tau with a sensitivity of 11.829 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

4 Images
Sandwich ELISA - Human N-368 Tau ELISA Kit- Extracellular (AB315060)
  • sELISA

Supplier Data

Sandwich ELISA - Human N-368 Tau ELISA Kit- Extracellular (AB315060)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean N-368 Tau concentration of detected samples was determined to be 76.61 pg/mL with a range of undetected – 92.97 pg/mL.

Sandwich ELISA - Human N-368 Tau ELISA Kit- Extracellular (AB315060)
  • sELISA

Supplier Data

Sandwich ELISA - Human N-368 Tau ELISA Kit- Extracellular (AB315060)

Example of human N-368 Tau standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human N-368 Tau ELISA Kit- Extracellular (AB315060)
  • sELISA

Supplier Data

Sandwich ELISA - Human N-368 Tau ELISA Kit- Extracellular (AB315060)

Recombinant N-368 Tau was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are as follows : 25% human serum, 25% plasma (citrate), 25% plasma (EDTA), 25% plasma (heparin), 50% cell culture media, and 50% CSF.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human N-368 Tau ELISA Kit- Extracellular (AB315060)
  • sELISA

Supplier Data

Sandwich ELISA - Human N-368 Tau ELISA Kit- Extracellular (AB315060)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean N-368 Tau concentration of detected samples was determined to be 76.61 pg/mL with a range of undetected – 92.97 pg/mL.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Cerebral Spinal Fluid

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 11.829 pg/mL

Range

46.875 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Product details

Human N-368 Tau SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of N-368 Tau protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media, Cerebral Spinal Fluid. Quantitate Human N-368 Tau with 11.829 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "79 - 86 %", "average": "= 84" }, { "sample": "Citrate plasma", "range": "79 - 84 %", "average": "= 81" }, { "sample": "EDTA Plasma", "range": "78 - 82 %", "average": "= 80" }, { "sample": "Heparin Plasma", "range": "107 - 121 %", "average": "= 115" }, { "sample": "Cell culture media", "range": "83 - 89 %", "average": "= 86" }, { "sample": "Cerebral Spinal Fluid", "range": "92 - 96 %", "average": "= 94" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315060-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315060-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315060-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS", "number":"AB315060-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate", "number":"AB315060-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB315060-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human N-368 Tau Capture Antibody", "number":"AB315060-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human N-368 Tau Lyophilized Recombinant Protein", "number":"AB315060-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human N-368 Tau Detector Antibody", "number":"AB315060-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-Coated 96-Well Microplate", "number":"AB315060-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB315060-CMP10", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
style
left-column

Product protocols

style
left-column

Target data

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
See full target information MAPT
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
websiteProtocolBooklet
en
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com