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AB315059

Human N-368 Tau ELISA Kit- Intracellular

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Human N-368 Tau ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human N-368 Tau with a sensitivity of 33.085 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

3 Images
Sandwich ELISA - Human N-368 Tau ELISA Kit- Intracellular (AB315059)
  • sELISA

Supplier Data

Sandwich ELISA - Human N-368 Tau ELISA Kit- Intracellular (AB315059)

Example of human N-368 Tau standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human N-368 Tau ELISA Kit- Intracellular (AB315059)
  • sELISA

Supplier Data

Sandwich ELISA - Human N-368 Tau ELISA Kit- Intracellular (AB315059)

Interpolated concentration of native N-368 Tau was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 600 µg/mL SH-SY5Y cell extract, 40 µg/mL brain cortex tissue extract, and 240 µg/mL brain stem tissue extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human N-368 Tau ELISA Kit- Intracellular (AB315059)
  • sELISA

Supplier Data

Sandwich ELISA - Human N-368 Tau ELISA Kit- Intracellular (AB315059)

The concentration of N-368 Tau was measured in 5-40 µg/mL of mouse brain tissue extract and in 30-60 µg/mL of rat brain tissue extract in duplicate.  The interpolated dilution factor corrected values are plotted as pg of N-368 Tau per µg of extract (mean +/- SD). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 33.085 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human N-368 Tau SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of N-368 Tau protein in Tissue extracts and Cell Lysate. Quantitate Human N-368 Tau with 33.085 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.1" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.3" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "92 - 106 %", "average": "= 100" }, { "sample": "Cell Lysate", "range": "84 - 91 %", "average": "= 87" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315059-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315059-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315059-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS", "number":"AB315059-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate", "number":"AB315059-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB315059-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human N-368 Tau Capture Antibody", "number":"AB315059-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human N-368 Tau Lyophilized Recombinant Protein", "number":"AB315059-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR", "number":"AB315059-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human N-368 Tau Detector Antibody", "number":"AB315059-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-Coated 96-Well Microplate", "number":"AB315059-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB315059-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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Product protocols

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Target data

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
See full target information MAPT
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websiteProtocolBooklet
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