AB254512

Human N-Cadherin ELISA Kit

Recombinant
SimpleStep
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(9 Publications)

Human N-Cadherin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human N-Cadherin with a sensitivity of 41 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

CD325, CDHN, NCAD, CDH2, Cadherin-2, CDw325, Neural cadherin, N-cadherin

3 Images

Sandwich ELISA - Human N-Cadherin ELISA Kit (AB254512)

sELISA

Supplier Data

Sandwich ELISA - Human N-Cadherin ELISA Kit (AB254512)

Interpolated concentrations of native N-Cadherin in human serum, plasma and cell culture supernatant samples.

The concentrations of N-Cadherin were measured in duplicates, interpolated from the N-Cadherin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (EDTA) 50%, plasma (heparin) 50% and HeLa cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean N-Cadherin concentration was determined to be 17,850 pg/mL in serum, 19,670 pg/mL in plasma (EDTA) and 12,801 pg/mL in plasma (heparin) and 8391 pg/mL in HeLa cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human N-Cadherin ELISA Kit (AB254512)

Interpolated concentrations of native N-Cadherin in human HeLa cell extract based on a 500 μg/mL extract load.

The concentrations of N-Cadherin were measured in duplicate and interpolated from the N-Cadherin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean N-Cadherin concentration was determined to be 13,895 pg/mL in HeLa cell extract.

sELISA

Supplier Data

Sandwich ELISA - Human N-Cadherin ELISA Kit (AB254512)

Example of human N-Cadherin standard curve in Sample Diluent NS.

The N-Cadherin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Cow, Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 41 pg/mL

Range

156.25 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human N-Cadherin ELISA Kit (ab254512) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of N-Cadherin protein in edta plasma, hep plasma, cell culture extracts, serum, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human N-Cadherin with 41 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

N-Cadherin is a member of the calcium-dependent adhesion molecule family of classical cadherins encoded by the CDH2 gene. This transmembrane, homophilic glycoprotein often can be found concentrated at adherens junctions in most cell types. The function of N-Cadherin depends much on the cellular contents such as cancer metastasis, interactions with catenins in neutral tissues, and maintaining the structural integrity of the heart by mediating strong adhesion between cardiomyocytes. This kit is raised as an extracellular fragment of N-Cadherin.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "81 - 102 %", "average": "= 92" }, { "sample": "Serum", "range": "113 - 115 %", "average": "= 114" }, { "sample": "EDTA Plasma", "range": "106 - 109 %", "average": "= 108" }, { "sample": "Cell culture extracts", "range": "84 - 108 %", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "89 - 95 %", "average": "= 92" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab254512-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB254512-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human N-Cadherin Detector Antibody", "number":"AB254512-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB254512-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB254512-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB254512-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB254512-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB254512-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB254512-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB254512-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human N-Cadherin Lyophilized Recombinant Protein", "number":"AB254512-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human N-Cadherin Capture Antibody", "number":"AB254512-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-Cadherin also known as CDH2 or neuronal cadherin is a calcium-dependent cell adhesion protein. It is characterized by its role in mediating intercellular connections primarily through its presence on neurons fibroblasts and certain epithelial cells. N-Cadherin has an approximate mass of 130 kDa. The protein is integral in forming homophilic interactions where N-Cadherin molecules on adjacent cells interact to establish robust cell-cell adhesion.

Biological function summary

N-Cadherin significantly influences cell-cell interaction and communication facilitating cellular adhesion and signal transduction. It is a vital component of adherens junctions contributing to tissue morphogenesis and stability. N-Cadherin interacts with other cytoplasmic proteins such as catenins forming a complex essential for linking the actin cytoskeleton to the cell membrane. This interaction affects cellular behaviors including migration and differentiation.

Pathways

N-Cadherin plays a significant role in the neural development and epithelial-to-mesenchymal transition (EMT) pathways important for development and cancer progression. It engages with related proteins such as beta-catenin which helps transduce signals within these pathways. N-Cadherin's interactions within these pathways highlight its role in maintaining multicellular structure and signaling processes important for development and pathogenesis.

N-Cadherin is associated with cancer and heart disease. In cancer particularly in the context of the EMT N-Cadherin facilitates tumor progression and metastasis cooperating with proteins like Twist and Snail to promote these processes. In heart disease alterations in N-Cadherin expression and function can impact cardiac muscle integrity and syncytium highlighting potential interactions with connexin43 to maintain cardiac function. Understanding these associations provides insights into therapeutic targets for these conditions.

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Product protocols

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Target data

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone : upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).

See full target information CDH2

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Mechanobiology in medicine 3:100099 PubMed40396132

2025

Mechanotransductive N-cadherin binding induces differentiation in human neural stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

McKay Cavanaugh,Rebecca Kuntz Willits

Discover oncology 15:157 PubMed38733530

2024

circPDK1 competitively binds miR-4731-5p to mediate GIGYF1 expression and increase paclitaxel sensitivity in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

YunYin Feng,TaoLong Zhang,Hong Liu

Evidence-based complementary and alternative medicine : eCAM 2022:4026688 PubMed36588592

2022

Caudatin Inhibits the Proliferation, Invasion, and Glycolysis of Osteosarcoma Cells via the Wnt/- Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Zhizhong Sheng,Chi Su,Yuli Xia,Xiangfan Chen,Xiaobo Huang,Honglin Li,Changsheng Ma,Ligang Wang

Frontiers in oncology 12:813809 PubMed35280775

2022

COL5A2 Inhibits the TGF-β and Wnt/β-Catenin Signaling Pathways to Inhibit the Invasion and Metastasis of Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Long Han,Dan Luo,Kakeng Habaxi,Julaiti Tayierjiang,Wei Zhao,Wei Wang,Wumaierjiang Aikebaier,Li Wang

BMC cancer 21:926 PubMed34399719

2021

Possible role of HPV/EBV coinfection in anoikis resistance and development in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Javid Sadri Nahand,Khadijeh Khanaliha,Hamed Mirzaei,Mohsen Moghoofei,Hossein Bannazadeh Baghi,Maryam Esghaei,Ali Reza Khatami,Maryam Fatemipour,Farah Bokharaei-Salim

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 30:727-736 PubMed34118143

2021

Baicalein modulates the radiosensitivity of cervical cancer cells in vitro via miR-183 and the JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwei Lei,Jingbin Shi,Yun Teng,Chenghui Song,Lijuan Zou,Fuxiu Ye,Haichen Zhang

Bioengineered 12:578-588 PubMed33517826

2021

Long non-coding RNA TTN antisense RNA 1 facilitates hepatocellular carcinoma progression via regulating miR-139-5p/SPOCK1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xinghao Zhu,Shiqing Jiang,Zongyao Wu,Tonghua Liu,Wei Zhang,Lili Wu,Lijun Xu,Mingliang Shao

Experimental and therapeutic medicine 21:116 PubMed33335579

2020

MicroRNA-451a prevents cutaneous squamous cell carcinoma progression via the 3-phosphoinositide-dependent protein kinase-1-mediated PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jixing Fu,Jianhua Zhao,Huamin Zhang,Xiaoli Fan,Wenjun Geng,Shaohua Qiao

Proceedings of SPIE--the International Society for 11251: PubMed32632340

2020

Prostate Cancer Epithelial Mesenchymal Transition by TGF-β1 exposure monitored with a Photonic Crystal Biosensor.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa Cadena,Frank DeLuna,Kwaku Baryeh,Lu-Zhe Sun,Jing Yong Ye

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human N-Cadherin ELISA Kit

Sandwich ELISA - Human N-Cadherin ELISA Kit (AB254512)

Sandwich ELISA - Human N-Cadherin ELISA Kit (AB254512)

Sandwich ELISA - Human N-Cadherin ELISA Kit (AB254512)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise