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Human N-Cadherin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human N-Cadherin in Cell culture extracts, Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

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Images

Sandwich ELISA - Human N-Cadherin ELISA Kit (AB254512), expandable thumbnail
  • Sandwich ELISA - Human N-Cadherin ELISA Kit (AB254512), expandable thumbnail
  • Sandwich ELISA - Human N-Cadherin ELISA Kit (AB254512), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Cow, Human

Range

156.25 - 10000 pg/mL

Assay time

1h 30m

Sensitivity

= 41 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

Alternative names

What's included?

1 x 96 Tests
Components
10X Human N-Cadherin Capture Antibody
1 x 600 µL
10X Human N-Cadherin Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 10 mL
Antibody Diluent 5BI
1 x 6 mL
Human N-Cadherin Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human N-Cadherin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human N-Cadherin in Cell culture extracts, Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Cow, Human

Range

156.25 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 41 pg/mL

Precision

Intra assay

Sample

Serum

n

8

mean

-

SD

-

C.V.

4.4

Inter assay

Sample

Serum

n

3

mean

-

SD

-

C.V.

9.8

Recovery

Sample specific recovery

Sample type

Cell culture supernatant

Average %

= 92

Range

81 - 102 %

Sample type

Serum

Average %

= 114

Range

113 - 115 %

Sample type

EDTA Plasma

Average %

= 108

Range

106 - 109 %

Sample type

Cell culture extracts

Average %

= 97

Range

84 - 108 %

Sample type

Heparin Plasma

Average %

= 92

Range

89 - 95 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human N-Cadherin ELISA Kit (ab254512) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of N-Cadherin protein in edta plasma, hep plasma, cell culture extracts, serum, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human N-Cadherin with 41 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

N-Cadherin is a member of the calcium-dependent adhesion molecule family of classical cadherins encoded by the CDH2 gene. This transmembrane, homophilic glycoprotein often can be found concentrated at adherens junctions in most cell types. The function of N-Cadherin depends much on the cellular contents such as cancer metastasis, interactions with catenins in neutral tissues, and maintaining the structural integrity of the heart by mediating strong adhesion between cardiomyocytes. This kit is raised as an extracellular fragment of N-Cadherin.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

N-Cadherin also known as CDH2 or neuronal cadherin is a calcium-dependent cell adhesion protein. It is characterized by its role in mediating intercellular connections primarily through its presence on neurons fibroblasts and certain epithelial cells. N-Cadherin has an approximate mass of 130 kDa. The protein is integral in forming homophilic interactions where N-Cadherin molecules on adjacent cells interact to establish robust cell-cell adhesion.

Biological function summary

N-Cadherin significantly influences cell-cell interaction and communication facilitating cellular adhesion and signal transduction. It is a vital component of adherens junctions contributing to tissue morphogenesis and stability. N-Cadherin interacts with other cytoplasmic proteins such as catenins forming a complex essential for linking the actin cytoskeleton to the cell membrane. This interaction affects cellular behaviors including migration and differentiation.

Pathways

N-Cadherin plays a significant role in the neural development and epithelial-to-mesenchymal transition (EMT) pathways important for development and cancer progression. It engages with related proteins such as beta-catenin which helps transduce signals within these pathways. N-Cadherin's interactions within these pathways highlight its role in maintaining multicellular structure and signaling processes important for development and pathogenesis.

Associated diseases and disorders

N-Cadherin is associated with cancer and heart disease. In cancer particularly in the context of the EMT N-Cadherin facilitates tumor progression and metastasis cooperating with proteins like Twist and Snail to promote these processes. In heart disease alterations in N-Cadherin expression and function can impact cardiac muscle integrity and syncytium highlighting potential interactions with connexin43 to maintain cardiac function. Understanding these associations provides insights into therapeutic targets for these conditions.

Product promise

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Full details and terms and conditions can be found here:
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3 product images

  • Sandwich ELISA - Human N-Cadherin ELISA Kit (ab254512), expandable thumbnail

    Sandwich ELISA - Human N-Cadherin ELISA Kit (ab254512)

    Example of human N-Cadherin standard curve in Sample Diluent NS.

    The N-Cadherin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human N-Cadherin ELISA Kit (ab254512), expandable thumbnail

    Sandwich ELISA - Human N-Cadherin ELISA Kit (ab254512)

    Interpolated concentrations of native N-Cadherin in human serum, plasma and cell culture supernatant samples.

    The concentrations of N-Cadherin were measured in duplicates, interpolated from the N-Cadherin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 50% and HeLa cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean N-Cadherin concentration was determined to be 17,850 pg/mL in serum, 19,670 pg/mL in plasma (EDTA) and 12,801 pg/mL in plasma (heparin) and 8391 pg/mL in HeLa cell culture supernatant.

  • Sandwich ELISA - Human N-Cadherin ELISA Kit (ab254512), expandable thumbnail

    Sandwich ELISA - Human N-Cadherin ELISA Kit (ab254512)

    Interpolated concentrations of native N-Cadherin in human HeLa cell extract based on a 500 μg/mL extract load.

    The concentrations of N-Cadherin were measured in duplicate and interpolated from the N-Cadherin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean N-Cadherin concentration was determined to be 13,895 pg/mL in HeLa cell extract.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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