Human NADH dehydrogenase ELISA Kit (Complex I)

Product details

Human NADH Dehydrogenase (Complex I) ELISA kit (ab178011) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NADH Dehydrogenase protein in human cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human NADH Dehydrogenase with 430 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

NADH dehydrogenase (NADH: ubiquinone reductase (H+-translocating), Complex I) is the first enzyme of the oxidative phosphorylation (OXPHOS) system within the mitochondrial inner membrane. NADH dehydrogenase is a large protein complex of 950,000 MW made up of 45-46 different subunits. Seven of the subunits of the complex are encoded on mitochondrial DNA (mtDNA), the remaining subunits are nuclear encoded, made in the cytosol and translocated into the organelle for assembly at the inner membrane. The enzyme complex catalyses electron entry from NADH via a flavin (FMN) and several non-heme iron centers. Mutations in mtDNA, or nuclear DNA genes encoding NADH dehydrogenase subunits or assembly factors are a common cause of genetic OXPHOS defects. Mutations or loss of mtDNA may cause enzymatic dysfunction by disrupting enzyme assembly or alternatively by specifically affecting enzymatic activity with no effect on enzyme assembly.

NADH dehydrogenase (like Complex III) has been proposed as a site of superoxide 'leak' from the mitochondrial OXPHOS system. Altered functioning and increased superoxide production by this complex has been proposed to contribute to several neurological disorders including Parkinson's disease. Also there is evidence of NADH Dehydrogenase involvement in diabetes.