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AB119587

Human NCAM1 ELISA Kit (CD56)

5

(1 Review)

|

(4 Publications)

Human NCAM1 ELISA Kit (CD56) is a Sandwich (quantitative) ELISA for the measurement of Human NCAM1 (CD56) in Human in Biofluids, Cell/Tissue Extracts, Cell Culture Media samples.

View Alternative Names

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

1 Images
Sandwich ELISA - Human NCAM1 ELISA Kit (CD56) (AB119587)
  • sELISA

Supplier Data

Sandwich ELISA - Human NCAM1 ELISA Kit (CD56) (AB119587)

Representative Standard Curve using ab119587.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

78 - 5000 pg/mL

Assay Platform

Microplate

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Reactivity data

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Product details

Abcam's Human NCAM1 (CD56) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human NCAM1 (CD56) in cell culture supernatants, serum and plasma (heparin, EDTA).

A NCAM1 (CD56) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for NCAM1 (CD56) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human NCAM1 (CD56) amount of sample captured in plate.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.
Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.
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Product protocols

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Target data

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.. (Microbial infection) Acts as a receptor for rabies virus.. (Microbial infection) Acts as a receptor for Zika virus.
See full target information NCAM1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eadi3647 PubMed37713494

2023

ATP1A3 as a target for isolating neuron-specific extracellular vesicles from human brain and biofluids.

Applications

Unspecified application

Species

Unspecified reactive species

Yang You,Zhengrong Zhang,Nadia Sultana,Maria Ericsson,Yuka A Martens,Min Sun,Takahisa Kanekiyo,Seiko Ikezu,Scott A Shaffer,Tsuneya Ikezu

Aging clinical and experimental research 35:2563-2571 PubMed37658983

2023

Handgrip strength asymmetry as a new biomarker for sarcopenia and individual sarcopenia signatures.

Applications

Unspecified application

Species

Unspecified reactive species

Jedd Pratt,Ludmilla Pessanha,Marco Narici,Colin Boreham,Giuseppe De Vito

Journal of translational medicine 21:377 PubMed37301958

2023

Plasma proteome of Long-COVID patients indicates HIF-mediated vasculo-proliferative disease with impact on brain and heart function.

Applications

Unspecified application

Species

Unspecified reactive species

Cristiana Iosef,Michael J Knauer,Michael Nicholson,Logan R Van Nynatten,Gediminas Cepinskas,Sorin Draghici,Victor K M Han,Douglas D Fraser

Scientific reports 7:6138 PubMed28733594

2017

CD56 Is a Pathogen Recognition Receptor on Human Natural Killer Cells.

Applications

ELISA

Species

Human

Sabrina Ziegler,Esther Weiss,Anna-Lena Schmitt,Jan Schlegel,Anne Burgert,Ulrich Terpitz,Markus Sauer,Lorenzo Moretta,Simona Sivori,Ines Leonhardt,Oliver Kurzai,Hermann Einsele,Juergen Loeffler
View all publications
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