AB282310

Human NCR1/NKp46 ELISA Kit

Recombinant
SimpleStep
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Human NCR1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human NCR1 with a sensitivity of 1.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD335, LY94, NCR1, Natural cytotoxicity triggering receptor 1, Lymphocyte antigen 94 homolog, NK cell-activating receptor, Natural killer cell p46-related protein, NK-p46, NKp46, hNKp46

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Sandwich ELISA - Human NCR1/NKp46 ELISA Kit (AB282310)

sELISA

Supplier Data

Sandwich ELISA - Human NCR1/NKp46 ELISA Kit (AB282310)

Interpolated concentrations of native NCR1 in human serum, plasma (citrate, EDTA and heparin) and NK-92 supernatant samples.

The concentrations of NCR1 were measured in duplicates, interpolated from the NCR1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (EDTA) 100%, plasma (heparin) 100% and NK-92 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NCR1 concentration was determined to be 142 pg/mL in neat serum, 116 pg/mL in neat plasma (citrate), 123 pg/mL in neat plasma (EDTA), 132 pg/mL in neat plasma (heparin) and 669 pg/mL in neat NK-92 supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human NCR1/NKp46 ELISA Kit (AB282310)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NCR1 concentration was determined to be 104 pg/mL with a range of 38 – 241 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human NCR1/NKp46 ELISA Kit (AB282310)

Example of human NCR1 standard curve in Sample Diluent NS.

The NCR1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.7 pg/mL

Range

10.94 - 700 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human NCR1 ELISA kit (ab282310) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human NCR1 protein in human serum, plasma, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human NCR1 with 1.7 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Natural cytotoxicity triggering receptor 1(NCR1) is a plasma membrane cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer cells to mediate tumor cell lysis.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "101 - 107 %", "average": "= 104" }, { "sample": "Serum", "range": "93 - 99 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "90 - 99 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "95 - 98 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "80 - 85 %", "average": "= 82" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCR1 also known as NKp46 is a receptor mainly expressed on the surface of natural killer (NK) cells. Its primary role involves recognizing and binding to ligands present on target cells including tumor cells and virus-infected cells. NCR1 initiates the activation of NK cells leading to the destruction of these abnormal cells. The NCR1 protein has a molecular weight of approximately 46 kDa and is a part of the immunoglobulin superfamily. NCR1 is mainly expressed in the immune system specifically on NK cells which are an important component of the innate immune response.

Biological function summary

The function of NCR1 involves the recognition of stressed transformed or infected cells marking them for elimination by NK cells. It does not typically function as a part of a complex; rather it serves a somewhat autonomous role in cell signaling. However its interaction with other receptors on NK cells including DAP12 enhances its signaling capabilities. Through the activation of cascades NCR1 promotes the release of cytotoxic granules and the production of cytokines therefore mediating the immune response.

Pathways

NCR1 plays a significant role in the regulation of immune surveillance pathways. It is involved in the recognition and elimination pathways that modulate the immune response against infected or cancerous cells. NK cells with NCR1's involvement interact with receptors like NKG2D adjusting the NK cell's cytotoxic activity. These interactions coordinate the broader immune response important for innate immunity effectively bridging innate and adaptive immunity by influencing other immune cells.

NCR1 has been implicated in cancer and infectious diseases. In cancer altered expression or dysfunction of NCR1 may contribute to the evasion of immune surveillance by tumor cells potentially leading to tumor progression. Studies have shown reduced NKp46 expression in some cancerous states linking it to poor prognosis. In infectious diseases NCR1 plays a role in targeting virus-infected cells and its dysfunction can result in reduced effectiveness in clearing viral infections. Interactions with viral envelope proteins can interfere with NCR1 activity exemplifying its importance in antiviral defense mechanisms.

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Product protocols

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Target data

Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.

See full target information NCR1

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human NCR1/NKp46 ELISA Kit

Sandwich ELISA - Human NCR1/NKp46 ELISA Kit (AB282310)

Sandwich ELISA - Human NCR1/NKp46 ELISA Kit (AB282310)

Sandwich ELISA - Human NCR1/NKp46 ELISA Kit (AB282310)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise