JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB264607

Human Nectin-4 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Nectin-4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Nectin-4 with a sensitivity of 98.62 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

LNIR, PRR4, PVRL4, NECTIN4, Nectin-4, Ig superfamily receptor LNIR, Nectin cell adhesion molecule 4, Poliovirus receptor-related protein 4

3 Images
Sandwich ELISA - Human Nectin-4 ELISA Kit (AB264607)
  • sELISA

Supplier Data

Sandwich ELISA - Human Nectin-4 ELISA Kit (AB264607)

Interpolated concentrations of native Nectin-4 in human pooled sex urine samples.

The concentrations of Nectin-4 were measured in duplicates, interpolated from the Nectin-4 standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nectin-4 concentration was determined to be 7.34 ng/mL in urine.

Sandwich ELISA - Human Nectin-4 ELISA Kit (AB264607)
  • sELISA

Supplier Data

Sandwich ELISA - Human Nectin-4 ELISA Kit (AB264607)

Interpolated concentrations of native Nectin-4 in human MCF7 based on a 62.50 μg/mL extract load.

The concentrations of Nectin-4 were measured in duplicate and interpolated from the Nectin-4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nectin-4 concentration was determined to be 1.44 ng/mL in MCF7 cell culture extract.

Sandwich ELISA - Human Nectin-4 ELISA Kit (AB264607)
  • sELISA

Supplier Data

Sandwich ELISA - Human Nectin-4 ELISA Kit (AB264607)

Example of human Nectin-4 standard curve in Sample Diluent 25BS + Enhancer.

The Nectin-4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Cell culture supernatant, Serum, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 98.62 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB192033

Anti-Nectin-4 antibody [EPR15613-68]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-Nectin-4 antibody [EPR15613-68] (AB192033)

  • 0
  • 0
Assay Kits

AB200016

Human Cardiac Troponin I ELISA Kit

Recombinant|SimpleStep

ELISA - Human Cardiac Troponin I ELISA Kit (AB200016)

  • 5
  • 2
Assay Kits

AB264632

Human EpCAM ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human EpCAM ELISA Kit (AB264632)

  • 0
  • 0
Assay Kits

AB260052

Human Alpha-synuclein ELISA Kit

KO Validated|Recombinant|SimpleStep

ELISA - Human Alpha-synuclein ELISA Kit (AB260052)

  • 5
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Nectin-4 ELISA Kit (ab264607) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Nectin-4 protein in cell lysate, hep plasma, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Nectin-4 with 98.62 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Nectin-4 is a cell adhesion molecule located in the epithelial adherens junctions. Mutations in Nectin-4 lead to Ectodermal dysplasia-syndactyly syndrome, a condition where there is abnormal development of two or more ectodermal structures (like hair, tooth and nails). A fragment of Nectin-4 is shed and circulates in serum in certain types of cancers. Additionally, Nectin-4 is also a receptor for the measles virus.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 99 %", "average": "= 93" }, { "sample": "Serum", "range": "82 - 98 %", "average": "= 89" }, { "sample": "Urine", "range": "85 - 98 %", "average": "= 89" }, { "sample": "Heparin Plasma", "range": "82 - 94 %", "average": "= 87" }, { "sample": "Cell Lysate", "range": "105 - 121 %", "average": "= 112" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab264607-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264607-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB264607-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264607-CMP13", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB264607-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264607-CMP14", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB264607-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB264607-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Nectin-4 Lyophilized Recombinant Protein", "number":"AB264607-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB264607-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Nectin-4 Capture Antibody", "number":"AB264607-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BS", "number":"AB264607-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB264607-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Nectin-4 Detector Antibody", "number":"AB264607-CMP11", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nectin-4 also known as PVRL4 is a member of the immunoglobulin-like adhesion molecule family. It has a molecular weight of approximately 66 kDa. Nectin-4 plays an important role in cell-cell adhesion by forming trans-dimers promoting cellular cohesion. It is expressed in tissues such as placenta embryonic tissues and several epithelial cancers indicating its potential involvement in developmental processes and pathological conditions. When utilizing methods such as 'nectin-4 ELISA' and 'nectin-4 IHC' researchers can study its expression extensively across different tissue samples.
Biological function summary

Nectin-4 functions as an important adhesion molecule that contributes to the maintenance and organization of cell junctions. It is often part of larger adhesive complexes that include afadin which links nectin proteins to the actin cytoskeleton stabilizing cell structure. Besides its role in maintaining cell-cell adhesion nectin-4 is significant in processes like cellular polarization and migration. These properties make it an important target for understanding tissue development and integrity as well as cancer metastasis.

Pathways

Nectin-4 belongs to the pathways regulating cell adhesion and the cytoskeletal organization. It is closely associated with the cadherin and integrin signaling pathways where it collaborates with other nectin family members and afadin to regulate these networks. These interactions impact cellular processes such as signal transduction and intercellular communication critical for tissue maintenance. Studies involving 'nectin expression' help further elucidate these pathway connections and their implications in healthy and diseased tissues.

Nectin-4 shows significant correlation with certain cancers such as breast and lung carcinoma. Its elevated expression in tumor tissues suggests a role in tumorigenesis and cancer progression. The protein's interaction with oncogenes like HER2 indicates a possible pathway by which nectin-4 affects cancer cell proliferation and survival. Understanding 'nectin-4 expression' in the context of these diseases aids in identifying potential biomarkers and therapeutic targets for cancer diagnosis and treatment.
style
left-column

Product protocols

style
left-column

Target data

Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.. (Microbial infection) Acts as a receptor for measles virus.
See full target information NECTIN4
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com