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AB288171

Human NEFL ELISA Kit

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Human NEFL ELISA Kit is a single-wash 90-min Simplestep used to quantify Human NEFL with a sensitivity of 30.785 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

NF68, NFL, NEFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

3 Images
ELISA - Human NEFL ELISA Kit (AB288171)
  • ELISA

Supplier Data

ELISA - Human NEFL ELISA Kit (AB288171)
Sandwich ELISA - Human NEFL ELISA Kit (AB288171)
  • sELISA

Supplier Data

Sandwich ELISA - Human NEFL ELISA Kit (AB288171)

Interpolated concentrations of spiked human NEFL in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Recombinant NEFL was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 100% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human NEFL ELISA Kit (AB288171)
  • sELISA

Supplier Data

Sandwich ELISA - Human NEFL ELISA Kit (AB288171)

Example of human NEFL standard curve in Sample Diluent NS. Example of human NEFL standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 30.785 pg/mL

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human NEFL ELISA Kit ab288171 is a rapid single-wash 90-min Sandwich ELISA to measure Human NEFL in EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 30.785 pg/mL.

How the assay works

Human NEFL SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human NEFL ELISA Kit ab288171 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-NEFL antibody [EPR23733-137] - BSA and Azide free (Capture) ab280028
- Anti-NEFL antibody [EPR23733-154] - BSA and Azide free (Detector) ab280028
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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.8" } ]
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Recovery

[ { "sample": "Serum", "range": "113 - 118 %", "average": "= 116" }, { "sample": "EDTA Plasma", "range": "105 - 119 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "99 - 117 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "113 - 116 %", "average": "= 115" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab288171-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB288171-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB288171-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB288171-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human NEFL Lyophilized Recombinant Protein", "number":"AB288171-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human NEFL Capture Antibody", "number":"AB288171-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB288171-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"10X Human NEFL Detector Antibody", "number":"AB288171-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB288171-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB288171-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB288171-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NEFL also known as neurofilament light polypeptide is a component of the cytoskeleton of neurons. It is one of the subunits of neurofilaments which also include medium and heavy chains. NEFL has a molecular mass of about 61.5 kDa. The protein is predominantly expressed in neurons throughout the central and peripheral nervous systems providing structural support and regulating axon diameter.
Biological function summary

Neurofilament light polypeptide forms heteropolymers with other neurofilament proteins contributing to the assembly of stable neurofilament fibers. These fibers maintain neuronal integrity and proper conductance velocities of nerve signals. NEFL interacts closely with neurofilament medium and heavy chains playing a role in the structural framework of the neuron. This enables efficient transmission of electrical impulses along axons.

Pathways

NEFL plays a significant role in the neurofilament pathway which is critical for neuronal cytoskeleton assembly and axonal transport. This pathway involves proteins such as NEFM (neurofilament medium) and NEFH (neurofilament heavy). NEFL's involvement in maintaining axonal caliber connects it to important signaling cascades like the axon guidance and neurotransmitter release pathways enhancing neuronal communication.

Defects in NEFL can lead to neurological conditions such as Charcot-Marie-Tooth disease type 2E and amyotrophic lateral sclerosis. Mutations or disruptions in NEFL impact axonal transport and neurofilament assembly contributing to neurodegeneration. NEFL is often studied alongside proteins like peripherin which can also exhibit mutations linked to similar neuropathies underlining its importance in neural health.
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Product protocols

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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
See full target information NEFL
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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