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Human NEFL ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human NEFL in Heparin Plasma, Serum, EDTA Plasma samples.

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Images

Sandwich ELISA - Human NEFL ELISA Kit (AB288171), expandable thumbnail
  • Sandwich ELISA - Human NEFL ELISA Kit (AB288171), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Sensitivity

= 30.785 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Target data

Function

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

Alternative names

What's included?

1 x 96 Tests
Components
10X Human NEFL Capture Antibody
1 x 600 µL
10X Human NEFL Detector Antibody
2 x 1 Vial
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent 5BI
1 x 6 mL
Human NEFL Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human NEFL ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human NEFL in Heparin Plasma, Serum, EDTA Plasma samples.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 30.785 pg/mL

Precision

Intra assay

Sample

Serum

n
8
mean
-
SD
-
C.V.

7.8

Inter assay

Sample

Serum

n
3
mean
-
SD
-
C.V.

11

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 116

Range

113 - 118 %

Sample type

EDTA Plasma

Average %

= 110

Range

105 - 119 %

Sample type

Heparin Plasma

Average %

= 106

Range

99 - 117 %

Sample type

Citrate plasma

Average %

= 115

Range

113 - 116 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human NEFL ELISA Kit (ab288171) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human NEFL protein in plasma and serum samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate NEFL with 30.785 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

NEFL also known as neurofilament light polypeptide is a component of the cytoskeleton of neurons. It is one of the subunits of neurofilaments which also include medium and heavy chains. NEFL has a molecular mass of about 61.5 kDa. The protein is predominantly expressed in neurons throughout the central and peripheral nervous systems providing structural support and regulating axon diameter.

Biological function summary

Neurofilament light polypeptide forms heteropolymers with other neurofilament proteins contributing to the assembly of stable neurofilament fibers. These fibers maintain neuronal integrity and proper conductance velocities of nerve signals. NEFL interacts closely with neurofilament medium and heavy chains playing a role in the structural framework of the neuron. This enables efficient transmission of electrical impulses along axons.

Pathways

NEFL plays a significant role in the neurofilament pathway which is critical for neuronal cytoskeleton assembly and axonal transport. This pathway involves proteins such as NEFM (neurofilament medium) and NEFH (neurofilament heavy). NEFL's involvement in maintaining axonal caliber connects it to important signaling cascades like the axon guidance and neurotransmitter release pathways enhancing neuronal communication.

Associated diseases and disorders

Defects in NEFL can lead to neurological conditions such as Charcot-Marie-Tooth disease type 2E and amyotrophic lateral sclerosis. Mutations or disruptions in NEFL impact axonal transport and neurofilament assembly contributing to neurodegeneration. NEFL is often studied alongside proteins like peripherin which can also exhibit mutations linked to similar neuropathies underlining its importance in neural health.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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2 product images

  • Sandwich ELISA - Human NEFL ELISA Kit (ab288171), expandable thumbnail

    Sandwich ELISA - Human NEFL ELISA Kit (ab288171)

    Interpolated concentrations of spiked human NEFL in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Recombinant NEFL was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 100% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - Human NEFL ELISA Kit (ab288171), expandable thumbnail

    Sandwich ELISA - Human NEFL ELISA Kit (ab288171)

    Example of human NEFL standard curve in Sample Diluent NS. Example of human NEFL standard curve. Background-subtracted data values (mean +/- SD) are graphed.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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