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AB309453

Human NELL-1 ELISA

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Human NELL-1 ELISA is a Sandwich (quantitative) ELISA for the measurement of Human NELL-1 ELISA in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

NRP1, NELL1, Protein kinase C-binding protein NELL1, NEL-like protein 1, Nel-related protein 1

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Sandwich ELISA - Human NELL-1 ELISA (AB309453)
  • sELISA

Supplier Data

Sandwich ELISA - Human NELL-1 ELISA (AB309453)

Example of standard curve using ab309453. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 10 pg/mL

Range

10 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Product details

Human NELL-1 ELISA is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human NELL-1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human NELL-1 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "97 - 137 %", "average": "= 114.3" }, { "sample": "Plasma", "range": "85 - 138 %", "average": "= 114.5" }, { "sample": "Cell culture supernatant", "range": "91 - 109 %", "average": "= 100.2" }, { "sample": "Cell culture media", "range": "91 - 109 %", "average": "= 100.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309453-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB309453-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB309453-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309453-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human NELL1 Detection Antibody", "number":"AB309453-CMP14", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human NELL1 Protein Standard", "number":"AB309453-CMP16", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human NELL1 Antibody-coated ELISA Plate", "number":"AB309453-CMP15", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB309453-CMP13", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309453-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309453-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NELL1 also known as Neural EGFL Like 1 is a protein primarily involved in cell signaling processes. Its molecular weight is approximately 87 kDa. NELL1 expresses in tissues such as bone cartilage blood vessels and the nervous system. It functions as a secreted glycoprotein and may play a role in mediating cellular communication.
Biological function summary

This protein influences cell growth differentiation and apoptosis. NELL1 is not known to be part of a multiprotein complex but it interacts with various factors that regulate tissue development. It has significant functions in osteogenesis and angiogenesis impacting bone formation and blood vessel formation. The presence of NELL1 is essential for normal skeletal development and repair.

Pathways

Multiple molecular interactions involve NELL1 in critical signaling pathways. NELL1 acts in the BMP signaling pathway an important player in bone and cartilage development. It also interacts with TGF-beta signaling pathways which regulate cell proliferation and differentiation. These interactions link NELL1 to proteins like BMP2 and TGF-beta receptors highlighting its role in cellular signaling dynamics.

NELL1 has associations with conditions like craniosynostosis and osteoporosis. Altered expression of NELL1 contributes to the premature fusion of skull bones in craniosynostosis. In osteoporosis NELL1 influences bone density and structure abnormalities. NELL1 also interacts with proteins such as RUNX2 which further contribute to the pathogenesis of these skeletal disorders.
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Product protocols

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Target data

Plays a role in the control of cell growth and differentiation. Promotes osteoblast cell differentiation and terminal mineralization.
See full target information NELL1
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB197315

Anti-NELL1 antibody

Western blot - Anti-NELL1 antibody (AB197315)

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Proteins & Peptides

AB116955

Recombinant Human NELL1 protein

SDS-PAGE - Recombinant Human NELL1 protein (AB116955)

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Product promise

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