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AB323694

Human Nephrin ELISA Kit- Intracellular

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Human Nephrin ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human Nephrin with a sensitivity of 11.1 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders

View Alternative Names

NPHN, NPHS1, Nephrin, Renal glomerulus-specific cell adhesion receptor

2 Images
Sandwich ELISA - Human Nephrin ELISA Kit- Intracellular (AB323694)
  • sELISA

Supplier Data

Sandwich ELISA - Human Nephrin ELISA Kit- Intracellular (AB323694)

Interpolated concentration of native Nephrin was measured in duplicate at different sample concentrations. Undiluted samples are 500 µg/mL human kidney tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human Nephrin ELISA Kit- Intracellular (AB323694)
  • sELISA

Supplier Data

Sandwich ELISA - Human Nephrin ELISA Kit- Intracellular (AB323694)

Example of human Nephrin standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 11.1 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Nephrin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Nephrin protein in Tissue extracts. Quantitate Human Nephrin with 11.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.8" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.9" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "101 - 105 %", "average": "= 103" } ]
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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab323694-10x96Tests", "assetComponentDetails": [ { "size":"10 x 600 µL", "name":"10X Human Nephrin Detector Antibody", "number":"AB323694-CMP02" }, { "size":"10 x 600 µL", "name":"10X Human Nephrin Capture Antibody", "number":"AB323694-CMP01" }, { "size":"20 x 1 Vial", "name":"Human Nephrin Lyophilized Recombinant Protein", "number":"AB323694-CMP03" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB323694-CMP09" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB323694-CMP08" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323694-CMP10" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323694-CMP06" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB323694-CMP05" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB323694-CMP04" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB323694-CMP11" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab323694-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human Nephrin Detector Antibody", "number":"AB323694-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Nephrin Capture Antibody", "number":"AB323694-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Nephrin Lyophilized Recombinant Protein", "number":"AB323694-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB323694-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB323694-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323694-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB323694-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323694-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB323694-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB323694-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323694-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).
See full target information NPHS1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com