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AB272199

Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein)

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Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein) is a single-wash 90-min Simplestep used to quantify Human Neurofilament H (200 kDa Neurofilament protein) with a sensitivity of 141 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

KIAA0845, NFH, NEFH, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

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Sandwich ELISA - Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein) (AB272199)
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Supplier Data

Sandwich ELISA - Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein) (AB272199)

Species reactivity was determined by measuring the protein in various samples, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations.

The mean Neurofilament H concentration was determined to be 5.301 μg/mg in human brain tissue extract, 3.386 μg/mg in mouse brain tissue extract, and 0.216 μg/mg in rat brain tissue extract.

Sandwich ELISA - Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein) (AB272199)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein) (AB272199)

Representative image of Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein) (ab272199)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human Neurofilament H (200 kDa Neurofilament protein) Capture Antibody

- 10X Human Neurofilament H (200 kDa Neurofilament protein) Detector Antibody

- Human Neurofilament H (200 kDa Neurofilament protein) Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein) (AB272199)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein) (AB272199)

Interpolated concentrations of native Neurofilament H in human SH-SY5Y cell extract based on a 250 µg/mL extract load and human brain tissue extract based on a 10 μg/mL extract load.

The concentrations of Neurofilament H were measured in duplicate and interpolated from the Neurofilament H standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neurofilament H concentration was determined to be 22.94 ng/mL in SH-SY5Y cell extract and 57.07 ng/mL in human brain tissue extract.

Sandwich ELISA - Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein) (AB272199)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein) (AB272199)

Example of human Neurofilament H standard curve in 1X Cell Extraction Buffer PTR + Enhancer.

The Neurofilament H standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 141 pg/mL

Range

0.94 - 60 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Neurofilament H ELISA kit (ab272199) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Neurofilament H protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Neurofilament H with 1.41 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Neurofilament H, also known as 200 kDa Neurofilament Protein, together with Neurofilament L and M are three intermediate filament proteins of neurofilaments. Neurofilament H is involved in the maintenance of neuronal caliber and may play a role in intracellular transport. Neurofilament H is used as a neuronal damage biomarker and mutations to the gene have been associated with susceptibility to amyotrophic lateral sclerosis. The human Neurofilament H amino acids sequence is 75% identical to mouse and rat Neurofilament H sequence.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "115 - 127 %", "average": "= 120" }, { "sample": "Cell Lysate", "range": "82 - 90 %", "average": "= 86" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272199-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB272199-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB272199-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB272199-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB272199-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB272199-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB272199-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Neurofilament H (200 kDa Neurofilament Protein) Lyophilized Recombinant Protein", "number":"AB272199-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Neurofilament H (200 kDa Neurofilament Protein) Capture Antibody", "number":"AB272199-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB272199-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Neurofilament H (200 kDa Neurofilament Protein) Detector Antibody", "number":"AB272199-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB272199-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB272199-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurofilament heavy polypeptide often referred to as NF-H or neurofilament heavy chain plays an important role in the structural integrity of neurons. This protein is a critical component of the cytoskeleton in neurons consisting of an approximate molecular mass of 200 kDa. It is predominantly expressed in the neuronal axons where it contributes to the assembly and stability of neurofilament structures. NF-H is essential for maintaining axonal diameter and for proper conduction of electrical impulses along nerve fibers.
Biological function summary

This polypeptide participates in the formation of neurofilament a complex structure that includes other neurofilament proteins like the neurofilament light (NF-L) and neurofilament medium (NF-M). These proteins form a stable scaffold within the axon aiding in neuronal growth and repair. The phosphorylation of NF-H modulates its interactions within the neurofilament network influencing axonal transport processes and dynamics which are vital for neuron function and survival.

Pathways

Neurofilament heavy polypeptide is integral to the axonal transport pathway important in conveying molecules between the neuronal cell body and the synaptic terminals. Through its interactions with microtubules and motor proteins NF-H facilitates the movement of organelles and neurotransmitters. It has significant associations with proteins involved in the ubiquitin-proteasome system reflecting its role in maintaining neuronal homeostasis and proteostasis essential in neuronal development and function.

Neurofilament heavy polypeptide serves as an important biomarker for neurodegenerative disorders such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. NF-H especially in phosphorylated form accumulates in conditions of axonal injury or degeneration. It often interacts with tau protein in Alzheimer's pathology forming neurofibrillary tangles. Elevated levels of NF-H in cerebrospinal fluid or blood can indicate neuronal damage offering insights into disease progression and potential targets for therapeutic intervention.
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Product protocols

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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
See full target information NEFH
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websiteProtocolBooklet
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