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Human Neurogranin ELISA Kit (NRGN) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Neurogranin (NRGN) in Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

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Images

Sandwich ELISA - Human Neurogranin ELISA Kit (NRGN) (AB277083), expandable thumbnail
  • Sandwich ELISA - Human Neurogranin ELISA Kit (NRGN) (AB277083), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

9.375 - 600 pg/mL

Assay time

1h 30m

Sensitivity

= 1.4 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Acts as a 'third messenger' substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium (By similarity).

Alternative names

What's included?

1 x 96 Tests
Components
10X Human Neurogranin (NRGN) Capture Antibody
1 x 600 µL
10X Human Neurogranin (NRGN) Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent 4BI
1 x 6 mL
Human Neurogranin (NRGN) Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human Neurogranin ELISA Kit (NRGN) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Neurogranin (NRGN) in Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

9.375 - 600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 1.4 pg/mL

Precision

Intra assay

Sample

Plasma

n
8
mean
-
SD
-
C.V.

7.2

Inter assay

Sample

Plasma

n
3
mean
-
SD
-
C.V.

14.9

Recovery

Sample specific recovery

Sample type

Cell culture supernatant

Average %

= 76

Range

72 - 78 %

Sample type

Serum

Average %

= 86

Range

80 - 93 %

Sample type

EDTA Plasma

Average %

= 96

Range

88 - 103 %

Sample type

Citrate plasma

Average %

= 104

Range

98 - 109 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human Neurogranin ELISA kit (ab277083) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Neurogranin protein in human serum, plasma - citrate, plasma EDTA, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Neurogranin with 1.4 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Neurogranin (NRGN), encoded by the NRGN gene, is a postsynaptic protein kinase that is highly expressed in the brain and regulates the availability of calmodulin; neurogranin binds to calmodulin in the absence of calcium. Phosphorylation by protein kinase C has been observed to decrease the calmodulin binding activity. Studies have previously shown that NRGN expression is regulated during development by thyroid hormones and retinoids for proper development of the central nervous system. ab277083 has been developed to specifically recognize full length neurogranin.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Neurogranin also known as NRGN is a small neuronal protein with an approximate molecular mass of 7.6 kDa. It is mainly expressed in the brain predominantly within the dendrites of neurons. Neurogranin binds to calmodulin in the presence of calcium playing an important role in synaptic plasticity. This protein acts as a kinase substrate influencing signal transduction pathways tied to learning and memory. Due to its specific expression pattern neurogranin serves as a significant molecular marker for studying the nervous system.

Biological function summary

Neurogranin functions as a modulator in cerebellar signaling and synaptic transmission. It influences calcium-calmodulin signaling facilitating the activation of proteins important in the central nervous system. Neurogranin does not form part of a multi-protein complex but interacts closely with calmodulin to regulate downstream signal cascades. These interactions emphasize its fundamental role in modulating processes that influence cognitive functions like learning and memory.

Pathways

Neurogranin significantly impacts the calcium signaling pathway and contributes to the regulation of long-term potentiation. It closely associates with proteins such as Protein Kinase C (PKC) and calmodulin-dependent protein kinases which are instrumental in these pathways. By modulating these essential signaling routes neurogranin affects intracellular signals that are vital for synaptic plasticity and enhanced neural communication.

Associated diseases and disorders

Neurogranin has a noted involvement in conditions like Alzheimer's disease and cognitive impairment. Changes in neurogranin expression levels are linked to the progression of Alzheimer's where reduced levels may contribute to the disruption of synaptic plasticity. Interactions with amyloid-beta peptides and other proteins such as tau highlight its potential as a biomarker for neurodegenerative diseases. These connections underline neurogranin's significant role in understanding cognitive-related disorders and guiding therapeutic research.

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2 product images

  • Sandwich ELISA - Human Neurogranin ELISA Kit (NRGN) (ab277083), expandable thumbnail

    Sandwich ELISA - Human Neurogranin ELISA Kit (NRGN) (ab277083)

    Example of human Neurogranin standard curve in Sample Diluent NS.

    The Neurogranin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human Neurogranin ELISA Kit (NRGN) (ab277083), expandable thumbnail

    Sandwich ELISA - Human Neurogranin ELISA Kit (NRGN) (ab277083)

    Interpolated concentrations of native Neurogranin in human plasma samples.

    The concentrations of Neurogranin were measured in duplicates, interpolated from the Neurogranin standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 100% and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neurogranin concentration was determined to be 77 pg/mL in plasma (citrate).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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