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AB277083

Human Neurogranin ELISA Kit (NRGN)

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Human Neurogranin ELISA Kit (NRGN) is a single-wash 90-min Simplestep used to quantify Human Neurogranin (NRGN) with a sensitivity of 1.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Neurogranin, Ng, RC3, NRGN

3 Images
ELISA - Human Neurogranin ELISA Kit (NRGN) (AB277083)
  • ELISA

Supplier Data

ELISA - Human Neurogranin ELISA Kit (NRGN) (AB277083)
Sandwich ELISA - Human Neurogranin ELISA Kit (NRGN) (AB277083)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neurogranin ELISA Kit (NRGN) (AB277083)

Interpolated concentrations of native Neurogranin in human plasma samples.

The concentrations of Neurogranin were measured in duplicates, interpolated from the Neurogranin standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma (citrate) 100% and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neurogranin concentration was determined to be 77 pg/mL in plasma (citrate).

Sandwich ELISA - Human Neurogranin ELISA Kit (NRGN) (AB277083)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neurogranin ELISA Kit (NRGN) (AB277083)

Example of human Neurogranin standard curve in Sample Diluent NS.

The Neurogranin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.4 pg/mL

Range

9.375 - 600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Neurogranin ELISA Kit (NRGN) ab277083 is a rapid single-wash 90-min Sandwich ELISA to measure Human Neurogranin (NRGN)in cell culture supernatant, citrate plasma, EDTA plasma, serum. This SimpleStep sensitivity is 1.4 pg/mL.

How the assay works

Human Neurogranin (NRGN)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Neurogranin ELISA Kit (NRGN) ab277083 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "14.9" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.2" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "72 - 78 %", "average": "= 76" }, { "sample": "Serum", "range": "80 - 93 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "88 - 103 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "98 - 109 %", "average": "= 104" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277083-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB277083-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB277083-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB277083-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB277083-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB277083-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Neurogranin (NRGN) Capture Antibody", "number":"AB277083-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Neurogranin (NRGN) Lyophilized Recombinant Protein", "number":"AB277083-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB277083-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB277083-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Neurogranin (NRGN) Detector Antibody", "number":"AB277083-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurogranin also known as NRGN is a small neuronal protein with an approximate molecular mass of 7.6 kDa. It is mainly expressed in the brain predominantly within the dendrites of neurons. Neurogranin binds to calmodulin in the presence of calcium playing an important role in synaptic plasticity. This protein acts as a kinase substrate influencing signal transduction pathways tied to learning and memory. Due to its specific expression pattern neurogranin serves as a significant molecular marker for studying the nervous system.
Biological function summary

Neurogranin functions as a modulator in cerebellar signaling and synaptic transmission. It influences calcium-calmodulin signaling facilitating the activation of proteins important in the central nervous system. Neurogranin does not form part of a multi-protein complex but interacts closely with calmodulin to regulate downstream signal cascades. These interactions emphasize its fundamental role in modulating processes that influence cognitive functions like learning and memory.

Pathways

Neurogranin significantly impacts the calcium signaling pathway and contributes to the regulation of long-term potentiation. It closely associates with proteins such as Protein Kinase C (PKC) and calmodulin-dependent protein kinases which are instrumental in these pathways. By modulating these essential signaling routes neurogranin affects intracellular signals that are vital for synaptic plasticity and enhanced neural communication.

Neurogranin has a noted involvement in conditions like Alzheimer's disease and cognitive impairment. Changes in neurogranin expression levels are linked to the progression of Alzheimer's where reduced levels may contribute to the disruption of synaptic plasticity. Interactions with amyloid-beta peptides and other proteins such as tau highlight its potential as a biomarker for neurodegenerative diseases. These connections underline neurogranin's significant role in understanding cognitive-related disorders and guiding therapeutic research.
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Product protocols

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Target data

Acts as a 'third messenger' substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium (By similarity).
See full target information NRGN
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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