Human Neurogranin ELISA Kit (NRGN) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Neurogranin (NRGN) in Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Colorimetric
Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Human
9.375 - 600 pg/mL
1h 30m
= 1.4 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Acts as a 'third messenger' substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium (By similarity).
Neurogranin, Ng, RC3, NRGN
Human Neurogranin ELISA Kit (NRGN) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Neurogranin (NRGN) in Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Colorimetric
Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Human
9.375 - 600 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 1.4 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Plasma | n 8 | mean - | SD - | C.V. 7.2 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Plasma | n 3 | mean - | SD - | C.V. 14.9 |
Sample type | Average % | Range |
---|---|---|
Sample type Cell culture supernatant | Average % = 76 | Range 72 - 78 % |
Sample type Serum | Average % = 86 | Range 80 - 93 % |
Sample type EDTA Plasma | Average % = 96 | Range 88 - 103 % |
Sample type Citrate plasma | Average % = 104 | Range 98 - 109 % |
Blue Ice
+4°C
+4°C
+4°C
Human Neurogranin ELISA kit (ab277083) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Neurogranin protein in human serum, plasma - citrate, plasma EDTA, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Neurogranin with 1.4 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Neurogranin (NRGN), encoded by the NRGN gene, is a postsynaptic protein kinase that is highly expressed in the brain and regulates the availability of calmodulin; neurogranin binds to calmodulin in the absence of calcium. Phosphorylation by protein kinase C has been observed to decrease the calmodulin binding activity. Studies have previously shown that NRGN expression is regulated during development by thyroid hormones and retinoids for proper development of the central nervous system. ab277083 has been developed to specifically recognize full length neurogranin.
This supplementary information is collated from multiple sources and compiled automatically.
Neurogranin also known as NRGN is a small neuronal protein with an approximate molecular mass of 7.6 kDa. It is mainly expressed in the brain predominantly within the dendrites of neurons. Neurogranin binds to calmodulin in the presence of calcium playing an important role in synaptic plasticity. This protein acts as a kinase substrate influencing signal transduction pathways tied to learning and memory. Due to its specific expression pattern neurogranin serves as a significant molecular marker for studying the nervous system.
Neurogranin functions as a modulator in cerebellar signaling and synaptic transmission. It influences calcium-calmodulin signaling facilitating the activation of proteins important in the central nervous system. Neurogranin does not form part of a multi-protein complex but interacts closely with calmodulin to regulate downstream signal cascades. These interactions emphasize its fundamental role in modulating processes that influence cognitive functions like learning and memory.
Neurogranin significantly impacts the calcium signaling pathway and contributes to the regulation of long-term potentiation. It closely associates with proteins such as Protein Kinase C (PKC) and calmodulin-dependent protein kinases which are instrumental in these pathways. By modulating these essential signaling routes neurogranin affects intracellular signals that are vital for synaptic plasticity and enhanced neural communication.
Neurogranin has a noted involvement in conditions like Alzheimer's disease and cognitive impairment. Changes in neurogranin expression levels are linked to the progression of Alzheimer's where reduced levels may contribute to the disruption of synaptic plasticity. Interactions with amyloid-beta peptides and other proteins such as tau highlight its potential as a biomarker for neurodegenerative diseases. These connections underline neurogranin's significant role in understanding cognitive-related disorders and guiding therapeutic research.
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Example of human Neurogranin standard curve in Sample Diluent NS.
The Neurogranin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of native Neurogranin in human plasma samples.
The concentrations of Neurogranin were measured in duplicates, interpolated from the Neurogranin standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 100% and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neurogranin concentration was determined to be 77 pg/mL in plasma (citrate).
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