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Human Neuron specific Enolase ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Neuron specific Enolase in Cerebral Spinal Fluid, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

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Images

Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (AB217778), expandable thumbnail
  • Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (AB217778), expandable thumbnail
  • Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (AB217778), expandable thumbnail
  • Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (AB217778), expandable thumbnail
  • Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (AB217778), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

312.5 - 20000 pg/mL

Assay time

1h 30m

Sensitivity

= 23 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).

Alternative names

What's included?

1 x 96 Tests
Components
10X Human Neuron-specific Enolase Capture Antibody
1 x 600 µL
10X Human Neuron-specific Enolase Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
50X Cell Extraction Enhancer Solution (ab193971)
1 x 1 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 10 mL
Antibody Diluent CPI2
1 x 6 mL
Human Neuron-specific Enolase Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human Neuron specific Enolase ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Neuron specific Enolase in Cerebral Spinal Fluid, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

312.5 - 20000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 23 pg/mL

Precision

Intra assay

Sample

Overall

n
5
mean
-
SD
-
C.V.

4.3

Inter assay

Sample

Overall

n
3
mean
-
SD
-
C.V.

11.4

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 106

Range

103 - 108 %

Sample type

EDTA Plasma

Average %

= 105

Range

96 - 118 %

Sample type

Cerebral Spinal Fluid

Average %

= 107

Range

103 - 110 %

Sample type

Cell culture extracts

Average %

= 90

Range

89 - 91 %

Sample type

Tissue Extracts

Average %

= 110

Range

106 - 113 %

Sample type

Heparin Plasma

Average %

= 109

Range

104 - 114 %

Sample type

Citrate plasma

Average %

= 102

Range

100 - 105 %

Sample type

Cell culture media

Average %

= 103

Range

102 - 105 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human Neuron specific Enolase ELISA Kit (ab217778) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Neuron specific Enolase protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Neuron specific Enolase with 23 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Neuron-specific Enolase (also known as NSE, gamma-enolase and Enolase 2) is a cytoplasmic phosphopyruvate hydratase. Neuron-specific Enolase has two related family members, Enolase 1 and Enolase 3. Neuron-specific Enolase can be used to identify neuronal cells and normal or malignant cells with neuroendocrine origin.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Neuron-specific enolase (NSE) also known simply as enolase-2 is a glycolytic enzyme with a molecular weight of approximately 47 kDa. This protein mainly facilitates the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. NSE is expressed significantly in neural tissues and neuroendocrine cells. Researchers often utilize it in various assays including NSE IHC and NSE ELISA to assess its expression levels in different tissue types.

Biological function summary

NSE plays a major role in cellular metabolism particularly in neurons where it aids energy production through glycolysis. It forms part of a dimeric complex bonding typically with itself or other enolase isoforms for proper function. As an important enzyme in the metabolic pathway NSE helps ensure neurons and associated cells maintain energy homeostasis which is fundamental for sustenance and normal cellular functions.

Pathways

NSE is an integral component of glycolysis a metabolic pathway pivotal in cellular energy production. The process collaborates closely with other glycolytic enzymes like pyruvate kinase and hexokinase to facilitate efficient energy release from glucose. Through these interactions NSE guarantees the smooth continuation of glycolysis highlighting its importance within cellular metabolism frameworks.

Associated diseases and disorders

NSE serves as a marker for neuroblastoma and small cell lung cancer. Elevated NSE levels suggest potential malignancies due to its release into the bloodstream from damaged neural and neuroendocrine cells. Moreover it often appears alongside proteins like neuron-specific markers such as synaptophysin in diagnostic assays. Understanding its involvement in these conditions helps clinicians diagnose and monitor the progression of certain cancers effectively.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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8 product images

  • Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (ab217778), expandable thumbnail

    Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (ab217778)

    Human Neuron specific Enolase standard curve comparison data.

    Standard curve comparison between human Neuron specific Enolase SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

  • Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (ab217778), expandable thumbnail

    Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (ab217778)

    Example of human Neuron-specific Enolase standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (ab217778), expandable thumbnail

    Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (ab217778)

    Example of human Neuron-specific Enolase standard curve in 1X Cell Extraction Buffer PTR.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (ab217778), expandable thumbnail

    Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (ab217778)

    Interpolated concentrations of spike Neuron-specific Enolase in human serum, and plasma samples.

    The concentrations of Neuron-specific Enolase were measured in duplicates, interpolated from the Neuron-specific Enolase standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (EDTA) 25%, plasma (heparin) 25%, and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (ab217778), expandable thumbnail

    Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (ab217778)

    Interpolated concentrations of native Neuron-specific Enolase in human serum, and plasma samples.

    The concentrations of Neuron-specific Enolase were measured in duplicates, interpolated from the Neuron-specific Enolase standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (EDTA) 25%, plasma (heparin) 25%, and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuron-specific Enolase concentration was determined to be 821 pg/mL in serum, 8545 pg/mL in plasma (EDTA), 1194 pg/mL in plasma (heparin), and 3386 pg/mL in plasma (citrate).

  • Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (ab217778), expandable thumbnail

    Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (ab217778)

    Serum from ten individual healthy human female donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Eight out of ten donors tested below the detectable dose. The mean Neuron-specific Enolase concentration of two donors was determined to be 1553 pg/mL with a range of 766 – 2340 pg/mL.

  • Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (ab217778), expandable thumbnail

    Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (ab217778)

    Interpolated concentrations of native Neuron-specific Enolase in human peripheral blood mononuclear (PBMC) cell culture supernatant samples.

    PBMCs were grown in the absence (unstimulated) or presence (stimulated) of phytohemagllutinin (PHA) for 3 days. The concentrations of Neuron-specific Enolase were measured in duplicates, interpolated from the Neuron-specific Enolase standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated 50%, and stimulated 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuron-specific Enolase concentration was determined to be undetectable in media, 711 pg/mL in unstimulated, and 4051 pg/mL in stimulated.

  • Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (ab217778), expandable thumbnail

    Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (ab217778)

    Interpolated concentrations of native Neuron-specific Enolase in human SH-SY5Y cell extract and human brain homogenate tissue extract samples based on a 50 μg/mL and 100 µg/mL extract load.

    The concentrations of Neuron-specific Enolase were measured in duplicate and interpolated from the Neuron-specific Enolase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuron-specific Enolase concentration was determined to be 5828 pg/mL in SH-SY5Y cell extract and 1482 pg/mL in human brain homogenate tissue extract.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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