AB217778

Human Neuron specific Enolase ELISA Kit

Recombinant
SimpleStep
What is this?

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(6 Publications)

Human Neuron specific Enolase ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Neuron specific Enolase with a sensitivity of 127 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2

7 Images

ELISA - Human Neuron specific Enolase ELISA Kit (AB217778)

ELISA

Supplier Data

ELISA - Human Neuron specific Enolase ELISA Kit (AB217778)

sELISA

Supplier Data

Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (AB217778)

Recombinant Neuron-specific Enolase was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 50% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (AB217778)

Example of human Neuron-specific Enolase standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (AB217778)

Standard curve comparison between Human Neuron-specific Enolase SimpleStep ELISA kit and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (AB217778)

Example of human Neuron-specific Enolase standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (AB217778)

Recombinant Neuron-specific Enolase was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 50% cell culture media and CSF. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Neuron specific Enolase ELISA Kit (AB217778)

Interpolated concentration of native Neuron-specific Enolase was measured in duplicate at different sample concentrations. Undiluted samples are 125 μg/mL Human hippocampus tissue extract, 12.5 μg/mL Human brain cortex tissue extract, and 100 μg/mL SH-SY5Y cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 127 pg/mL

Range

312.5 - 20000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Neuron specific Enolase ELISA Kit ab217778 is a rapid single-wash 90-min Sandwich ELISA to measure Human Neuron specific Enolase in cell culture extracts, cell culture supernatant, CSF, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 23 pg/mL.

How the assay works

Human Neuron specific Enolase SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Neuron specific Enolase ELISA Kit ab217778 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" } ]

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Recovery

[ { "sample": "Serum", "range": "88 - 105 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "101 - 104 %", "average": "= 95" }, { "sample": "Cerebral Spinal Fluid", "range": "86 - 109 %", "average": "= 96" }, { "sample": "Cell culture extracts", "range": "84 - 93 %", "average": "= 88" }, { "sample": "Tissue Extracts", "range": "86 - 109 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "95 - 113 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "83 - 102 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "100 - 105 %", "average": "= 103" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neuron-specific enolase (NSE) also known simply as enolase-2 is a glycolytic enzyme with a molecular weight of approximately 47 kDa. This protein mainly facilitates the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. NSE is expressed significantly in neural tissues and neuroendocrine cells. Researchers often utilize it in various assays including NSE IHC and NSE ELISA to assess its expression levels in different tissue types.

Biological function summary

NSE plays a major role in cellular metabolism particularly in neurons where it aids energy production through glycolysis. It forms part of a dimeric complex bonding typically with itself or other enolase isoforms for proper function. As an important enzyme in the metabolic pathway NSE helps ensure neurons and associated cells maintain energy homeostasis which is fundamental for sustenance and normal cellular functions.

Pathways

NSE is an integral component of glycolysis a metabolic pathway pivotal in cellular energy production. The process collaborates closely with other glycolytic enzymes like pyruvate kinase and hexokinase to facilitate efficient energy release from glucose. Through these interactions NSE guarantees the smooth continuation of glycolysis highlighting its importance within cellular metabolism frameworks.

NSE serves as a marker for neuroblastoma and small cell lung cancer. Elevated NSE levels suggest potential malignancies due to its release into the bloodstream from damaged neural and neuroendocrine cells. Moreover it often appears alongside proteins like neuron-specific markers such as synaptophysin in diagnostic assays. Understanding its involvement in these conditions helps clinicians diagnose and monitor the progression of certain cancers effectively.

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Product protocols

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Target data

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).

See full target information ENO2

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 21:488 PubMed38105218

2023

Inhibitory effects of the nanoscale lysate derived from xenogenic dental pulp stem cells in lung cancer models.

Applications

Unspecified application

Species

Unspecified reactive species

Yan He,Ruohan Li,Wenting She,Yilong Ai,Kesheng Li,Tushar Kumeria,Ziran Jiang,Qing Shao,Chen Zou,Abdullkhaleg Ali Albashari,Xingxiang Duan,Qingsong Ye

PloS one 18:e0279343 PubMed36800341

2023

Doublecortin and Glypican-2 concentrations in the cerebrospinal fluid from infants are developmentally downregulated.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine Brégère,Urs Fisch,Florian Samuel Halbeisen,Christian Schneider,Tanja Dittmar,Sarah Stricker,Soheila Aghlmandi,Raphael Guzman

American journal of translational research 14:6638-6646 PubMed36247290

2022

Treatment effects of monosialotetrahexosylganglioside on severe traumatic brain injury in adults.

Applications

Unspecified application

Species

Unspecified reactive species

Hanqing Chu,Jindan Gao

Stem cells international 2022:2744789 PubMed36106176

2022

Promoting Immortalized Adipose-Derived Stem Cell Transdifferentiation and Proliferation into Neuronal-Like Cells through Consecutive 525 nm and 825 nm Photobiomodulation.

Applications

Unspecified application

Species

Unspecified reactive species

Madeleen Jansen van Rensburg,Anine Crous,Heidi Abrahamse

Journal of Alzheimer's disease : JAD 69:871-880 PubMed31156178

2019

Immortalized Human Bone Marrow Derived Stromal Cells in Treatment of Transient Cerebral Ischemia in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jianyuan Li,Weidong Liu,Weicheng Yao

Molecular medicine reports 16:3201-3207 PubMed28714031

2017

Effects of neuritin on the differentiation of bone marrow‑derived mesenchymal stem cells into neuron‑like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jingling Zhu,Pingping Meng,Qian Wang,Haiyan Wang,Jinli Zhang,Yuanyuan Li,Dongzheng Li,Xiaohua Tan,Lei Yang,Jin Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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Associated Products

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