AB323496

Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Extracellular

SimpleStep
Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human Neuronal Pentraxin-1 (NP-I) with a sensitivity of 24.265 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Neuronal pentraxin-1, NP1, Neuronal pentraxin I, NP-I, NPTX1

7 Images

Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Extracellular (AB323496)

sELISA

Supplier Data

Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Extracellular (AB323496)

50 ng/mL of recombinant human NPTX2 and NPTXR were individually tested for cross reactivity. Percent reactivity was determined by interpolating the protein concentration from the human Neuronal Pentraxin-1 (NP-I/NPTX1) standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested. No cross reactivity was observed.

sELISA

Supplier Data

Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Extracellular (AB323496)

50 ng/mL of recombinant human NPTX2 and 50 ng/mL of recombinant human NPTXR were individually tested for interference with 1,150 pg/mL of recombinant human Neuronal Pentraxin-1 (NP-I/NPTX1). Percent reactivity was determined by interpolating the protein concentration from the human Neuronal Pentraxin-1 standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested. No significant interference was observed.

sELISA

Supplier Data

Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Extracellular (AB323496)

Interpolated concentration of native Neuronal Pentraxin-1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 20% cerebrospinal fluid. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Supplier Data

Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Extracellular (AB323496)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Neuronal Pentraxin-1 concentration was determined to be 1,922 pg/mL with a range of 818 – 4,218 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Extracellular (AB323496)

Example of human Neuronal Pentraxin-1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Extracellular (AB323496)

Example of human Neuronal Pentraxin-1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Extracellular (AB323496)

Interpolated concentration of native Neuronal Pentraxin-1 was measured in duplicate at different sample concentrations. Undiluted samples are 25% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Cerebral Spinal Fluid

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 24.265 pg/mL

Range

71.875 - 4600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

style

left-column

Product details

Human Neuronal Pentraxin-1 (NP-I) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Neuronal Pentraxin-1 (NP-I) protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media, Cerebral Spinal Fluid. Quantitate Human Neuronal Pentraxin-1 (NP-I) with 24.265 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

style

left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Cerebral Spinal Fluid", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.7" }, { "reproducibilityType": "Inter", "sample": "Cerebral Spinal Fluid", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.1" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "80 - 92 %", "average": "= 85" }, { "sample": "Citrate plasma", "range": "82 - 84 %", "average": "= 83" }, { "sample": "EDTA Plasma", "range": "76 - 85 %", "average": "= 81" }, { "sample": "Heparin Plasma", "range": "80 - 84 %", "average": "= 81" }, { "sample": "Cell culture media", "range": "92 - 99 %", "average": "= 97" }, { "sample": "Cerebral Spinal Fluid", "range": "94 - 102 %", "average": "= 98" } ]

style

left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab323496-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB323496-CMP08" }, { "size":"10 x 600 µL", "name":"10X Human Neuronal Pentraxin-1 (NP-I) Detector Antibody", "number":"AB323496-CMP02" }, { "size":"10 x 600 µL", "name":"10X Human Neuronal Pentraxin-1 (NP-I) Capture Antibody", "number":"AB323496-CMP01" }, { "size":"20 x 1 Vial", "name":"Human Neuronal Pentraxin-1 (NP-I) Lyophilized Recombinant Protein", "number":"AB323496-CMP03" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB323496-CMP07" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323496-CMP09" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323496-CMP05" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB323496-CMP10" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB323496-CMP04" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab323496-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB323496-CMP08" }, { "size":"1 x 600 µL", "name":"10X Human Neuronal Pentraxin-1 (NP-I) Detector Antibody", "number":"AB323496-CMP02" }, { "size":"1 x 600 µL", "name":"10X Human Neuronal Pentraxin-1 (NP-I) Capture Antibody", "number":"AB323496-CMP01" }, { "size":"2 x 1 Vial", "name":"Human Neuronal Pentraxin-1 (NP-I) Lyophilized Recombinant Protein", "number":"AB323496-CMP03" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB323496-CMP07" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB323496-CMP11" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB323496-CMP10" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB323496-CMP04" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323496-CMP05" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab323496-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB323496-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Neuronal Pentraxin-1 (NP-I) Detector Antibody", "number":"AB323496-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Neuronal Pentraxin-1 (NP-I) Capture Antibody", "number":"AB323496-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Neuronal Pentraxin-1 (NP-I) Lyophilized Recombinant Protein", "number":"AB323496-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323496-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB323496-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB323496-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB323496-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323496-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323496-CMP05", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

style

left-column

Target data

May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses.

See full target information NPTX1_HUMAN

style

left-column

style

left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

style

left-column

websiteProtocolBooklet

style

right-column

style

bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com