Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Neuronal Pentraxin-1 (NP-I) - Intracellular in Cell Lysate, Tissue Extracts samples.
Images
Key facts
- Detection method
Colorimetric
- Sample types
Cell Lysate, Tissue Extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
71.875 - 4600 pg/mL
- Assay time
1h 30m
- Sensitivity
= 23.263 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses.
Alternative names
Neuronal pentraxin-1, NP1, Neuronal pentraxin I, NP-I, NPTX1
What's included?
Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Neuronal Pentraxin-1 (NP-I) - Intracellular in Cell Lysate, Tissue Extracts samples.
Key facts
- Detection method
Colorimetric
- Sample types
Cell Lysate, Tissue Extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
71.875 - 4600 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 23.263 pg/mL
- General recovery
97%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. = 3.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. = 9.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 97 | Range 91 - 103 % |
Sample type Tissue Extracts | Average % = 103 | Range 100 - 107 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human Neuronal Pentraxin-1 (NP-I) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Neuronal Pentraxin-1 (NP-I) protein in Cell Lysate, Tissue extracts. Quantitate Human Neuronal Pentraxin-1 (NP-I) with 23.263 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples -96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Intracellular (ab323497)
50 ng/mL of recombinant human NPTX2 and 50 ng/mL of recombinant human NPTXR were individually tested for interference with 1,150 pg/mL of recombinant human Neuronal Pentraxin-1 (NP-I/NPTX1). Percent reactivity was determined by interpolating the protein concentration from the human Neuronal Pentraxin-1 standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested. No significant interference was observed.
Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Intracellular (ab323497)
50 ng/mL of recombinant human NPTX2 and NPTXR were individually tested for cross reactivity. Percent reactivity was determined by interpolating the protein concentration from the human Neuronal Pentraxin-1 (NP-I/NPTX1) standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested. No cross reactivity was observed.
Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Intracellular (ab323497)
Example of human Neuronal Pentraxin-1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Intracellular (ab323497)
Interpolated concentration of native Neuronal Pentraxin-1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: HeLa cell extract 500 μg/mL and hippocampus tissue extract 40 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.
Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Intracellular (ab323497)
Example of human Neuronal Pentraxin-1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Intracellular (ab323497)
Interpolated concentration of native Neuronal Pentraxin-1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 500 μg/mL HeLa cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.
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