AB323497

Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Intracellular

SimpleStep
Recombinant
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Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human Neuronal Pentraxin-1 (NP-I) with a sensitivity of 23.263 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Neuronal pentraxin-1, NP1, Neuronal pentraxin I, NP-I, NPTX1

6 Images

Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Intracellular (AB323497)

sELISA

Supplier Data

Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Intracellular (AB323497)

Example of human Neuronal Pentraxin-1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Intracellular (AB323497)

Example of human Neuronal Pentraxin-1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Intracellular (AB323497)

Interpolated concentration of native Neuronal Pentraxin-1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 500 μg/mL HeLa cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Supplier Data

Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Intracellular (AB323497)

50 ng/mL of recombinant human NPTX2 and 50 ng/mL of recombinant human NPTXR were individually tested for interference with 1,150 pg/mL of recombinant human Neuronal Pentraxin-1 (NP-I/NPTX1). Percent reactivity was determined by interpolating the protein concentration from the human Neuronal Pentraxin-1 standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested. No significant interference was observed.

sELISA

Supplier Data

Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Intracellular (AB323497)

Interpolated concentration of native Neuronal Pentraxin-1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HeLa cell extract 500 μg/mL and hippocampus tissue extract 40 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Supplier Data

Sandwich ELISA - Human Neuronal Pentraxin-1 (NP-I) ELISA Kit- Intracellular (AB323497)

50 ng/mL of recombinant human NPTX2 and NPTXR were individually tested for cross reactivity. Percent reactivity was determined by interpolating the protein concentration from the human Neuronal Pentraxin-1 (NP-I/NPTX1) standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested. No cross reactivity was observed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 23.263 pg/mL

Range

71.875 - 4600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Neuronal Pentraxin-1 (NP-I) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Neuronal Pentraxin-1 (NP-I) protein in Cell Lysate, Tissue extracts. Quantitate Human Neuronal Pentraxin-1 (NP-I) with 23.263 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples -96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.6" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.7" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "91 - 103 %", "average": "= 97" }, { "sample": "Tissue Extracts", "range": "100 - 107 %", "average": "= 103" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses.

See full target information NPTX1_HUMAN

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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