Human Neuropilin-1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Neuropilin-1 in Human in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.78 - 50 ng/mL
- Assay time
- 3h 30m
- Sensitivity
- < 10 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins (PubMed:10688880, PubMed:9288753, PubMed:9529250). Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage (PubMed:19805273). It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB (PubMed:10688880, PubMed:19805273, PubMed:9288753, PubMed:9529250). Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR (PubMed:30623799). (Microbial infection) Acts as a host factor for human coronavirus SARS-CoV-2 infection. Recognizes and binds to CendR motif RRAR on SARS-CoV-2 spike protein S1 which enhances SARS-CoV-2 infection. Isoform 2. Binds VEGF-165 and may inhibit its binding to cells (PubMed:10748121, PubMed:26503042). May induce apoptosis by sequestering VEGF-165 (PubMed:10748121). May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
Alternative names
CD304, NRP, VEGF165R, NRP1, Neuropilin-1, Vascular endothelial cell growth factor 165 receptor
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Human Neuropilin-1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Neuropilin-1 in Human in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.78 - 50 ng/mL
- Assay time
- 3h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- < 10 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample 1 | n 16 | mean 1.578 ng/mL | SD 0.069 | C.V. 4.4 |
Sample Sample 2 | n 16 | mean 4.783 ng/mL | SD 0.317 | C.V. 6.6 |
Sample Sample 3 | n 16 | mean 20.934 ng/mL | SD 0.921 | C.V. 4.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample 1 | n 24 | mean 1.501 ng/mL | SD 0.09 | C.V. 6 |
Sample Sample 2 | n 24 | mean 4.697 ng/mL | SD 0.371 | C.V. 7.9 |
Sample Sample 3 | n 24 | mean 20.813 ng/mL | SD 1.207 | C.V. 5.8 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
The Human Neuropilin-1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213813) is designed for the quantitative detection of Human Neuropilin-1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polyconal antibody from goat specific for Neuropilin-1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: F22-K644) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neuropilin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Neuropilin-1 amount of sample captured in plate.
NRP1 (Neuropilin 1) also known as NP1, NRP, BDCA4 or VEGF165R, is a membrane-bound co-receptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF) and semaphorin family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration and invasion. By somatic cell hybrid analysis, the NRP1 gene was mapped to chromosome 10. NRP1 bounds PGF1 with lower affinity. NRP1-mediated interactions are a necessary element in the initiation of the primary immune response and offer another example, like that of agrin, of a molecule shared by neurologic and immunologic synapses. After T-cell contact with DC, T-cell NRP1 colocalized with CD3 in the immunologic synapse and, sometimes, also at the opposite pole of the T cell. Soluble NRP1 interacts in a homophilic fashion with NRP1 on both DC and T cells, and this binding can be inhibited by blocking antibodies to NRP1. Furthermore, selective NRP1 inhibition in this model suppressed neovascular formation substantially.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Neuropilin-1 also known as NRP1 is a transmembrane protein with a significant role in the nervous and vascular systems. It has a molecular mass of approximately 130 kDa. Neuropilin-1 expression occurs broadly in tissues including neurons endothelial cells and tumor cells. Its structure includes a large extracellular domain that binds various ligands mediating several cellular functions. Neuropilin-1 is sometimes targeted in experiments using methods like neuropilin-1 ELISA and PE immunofluorescence to study its distribution and function in different tissues.
- Biological function summary
Neuropilin-1 serves as a co-receptor for both the Vascular Endothelial Growth Factor (VEGF) and Semaphorin family proteins. It plays an important role in processes such as angiogenesis axonal guidance and the immune system. Neuropilin-1 does not function alone; it forms complexes with neuropilin-2 and other receptors like Plexin and VEGFR enhancing signal transduction pathways for angiogenesis and neuronal development. This involvement allows cells to respond appropriately to their environment especially during organismal development and repair processes.
- Pathways
Neuropilin-1 facilitates interactions within the VEGF and Semaphorin pathways. In the VEGF pathway Neuropilin-1 enhances binding and signaling efficiency with VEGF closely working alongside VEGFR to promote endothelial cell survival migration and new blood vessel formation. In the Semaphorin pathway Neuropilin-1 interacts with Plexins mediating neuronal pathfinding and axonal growth. These interactions highlight Neuropilin-1's adaptive capabilities in various physiological processes critical for system development.
- Associated diseases and disorders
Neuropilin-1 is linked to pathological conditions like cancer and cardiovascular diseases. Neuropilin-1 overexpression is frequently observed in tumors driving cancer progression through enhanced angiogenesis and tissue invasion closely interacting with proteins like VEGF-A. In cardiovascular disease Neuropilin-1 contributes to abnormal blood vessel formation and stability. By studying Neuropilin-1 and other biomarkers like CD304 FITC researchers aim to develop therapeutic strategies targeting its role in these diseases.
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Sandwich ELISA - Human Neuropilin-1 ELISA Kit (ab213813)
Human Neuropilin-1 ELISA Kit (ab213813) Standard Curve
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