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AB227901

Human Neuropilin-1 ELISA Kit

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(2 Publications)

Human Neuropilin-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Neuropilin-1 with a sensitivity of 7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD304, NRP, VEGF165R, NRP1, Neuropilin-1, Vascular endothelial cell growth factor 165 receptor

7 Images
Sandwich ELISA - Human Neuropilin-1 ELISA Kit (AB227901)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neuropilin-1 ELISA Kit (AB227901)

Human Neuropilin-1 Standard Curve Data.

Standard curve comparison between human Neuropilin-1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human Neuropilin-1 ELISA Kit (AB227901)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neuropilin-1 ELISA Kit (AB227901)

Example of human Neuropilin-1 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Neuropilin-1 ELISA Kit (AB227901)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neuropilin-1 ELISA Kit (AB227901)

Example of human Neuropilin-1 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Neuropilin-1 ELISA Kit (AB227901)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neuropilin-1 ELISA Kit (AB227901)

Interpolated concentrations of native Neuropilin-1 in human serum and plasma samples.

The concentrations of Neuropilin-1 were measured in duplicates, interpolated from the Neuropilin-1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 200, plasma (citrate) 1 : 100, plasma (heparin) 1 : 100 and plasma (EDTA) 1 : 100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuropilin-1 concentration was determined to be 330.1 ng/mL in neat serum, 249.9 ng/mL in neat plasma (citrate), 232.5 ng/mL in neat plasma (heparin), and 268.1 ng/mL in neat plasma (EDTA).

Sandwich ELISA - Human Neuropilin-1 ELISA Kit (AB227901)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neuropilin-1 ELISA Kit (AB227901)

Interpolated concentrations of native Neuropilin-1 in human breast milk and urine samples.

The concentrations of Neuropilin-1 were measured in duplicates, interpolated from the Neuropilin-1 standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 1 : 50 and urine 1 : 8. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuropilin-1 concentration was determined to be 24.31 ng/mL in neat breast milk and 10.12 ng/mL in neat urine.

Sandwich ELISA - Human Neuropilin-1 ELISA Kit (AB227901)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neuropilin-1 ELISA Kit (AB227901)

Interpolated concentrations of native Neuropilin-1 in human HT-29 cell extract based on a 250 µg/mL extract load and human heart tissue extract based on a 125 μg/mL extract load.

The concentrations of Neuropilin-1 were measured in duplicate and interpolated from the Neuropilin-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuropilin-1 concentration was determined to be 2.982 ng/mL in HT-29 cell extract and 2.732 ng/mL in human heart tissue extract.

Sandwich ELISA - Human Neuropilin-1 ELISA Kit (AB227901)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neuropilin-1 ELISA Kit (AB227901)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuropilin-1 concentration was determined to be 702.5 ng/mL with a range of 533.6 – 937.3 ng/mL.

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Milk, Serum, Tissue Culture Media, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 7 pg/mL

Range

37.5 - 4800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Neuropilin-1 ELISA Kit (ab227901) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Neuropilin-1 protein in cit plasma, edta plasma, hep plasma, milk, serum, tissue culture media, tissue extracts, urine, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Neuropilin-1 with 7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Human Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" }, { "reproducibilityType": "Intra", "sample": "Human Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" } ]
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Recovery

[ { "sample": "Milk", "range": "84 - 97 %", "average": "= 89" }, { "sample": "Serum", "range": "107 - 113 %", "average": "= 110" }, { "sample": "EDTA Plasma", "range": "106 - 110 %", "average": "= 108" }, { "sample": "Cell culture extracts", "range": "86 - 105 %", "average": "= 95" }, { "sample": "Urine", "range": "81 - 85 %", "average": "= 83" }, { "sample": "Tissue Extracts", "range": "94 - 104 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "91 - 97 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "111 - 119 %", "average": "= 114" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neuropilin-1 also known as NRP1 is a transmembrane protein with a significant role in the nervous and vascular systems. It has a molecular mass of approximately 130 kDa. Neuropilin-1 expression occurs broadly in tissues including neurons endothelial cells and tumor cells. Its structure includes a large extracellular domain that binds various ligands mediating several cellular functions. Neuropilin-1 is sometimes targeted in experiments using methods like neuropilin-1 ELISA and PE immunofluorescence to study its distribution and function in different tissues.
Biological function summary

Neuropilin-1 serves as a co-receptor for both the Vascular Endothelial Growth Factor (VEGF) and Semaphorin family proteins. It plays an important role in processes such as angiogenesis axonal guidance and the immune system. Neuropilin-1 does not function alone; it forms complexes with neuropilin-2 and other receptors like Plexin and VEGFR enhancing signal transduction pathways for angiogenesis and neuronal development. This involvement allows cells to respond appropriately to their environment especially during organismal development and repair processes.

Pathways

Neuropilin-1 facilitates interactions within the VEGF and Semaphorin pathways. In the VEGF pathway Neuropilin-1 enhances binding and signaling efficiency with VEGF closely working alongside VEGFR to promote endothelial cell survival migration and new blood vessel formation. In the Semaphorin pathway Neuropilin-1 interacts with Plexins mediating neuronal pathfinding and axonal growth. These interactions highlight Neuropilin-1's adaptive capabilities in various physiological processes critical for system development.

Neuropilin-1 is linked to pathological conditions like cancer and cardiovascular diseases. Neuropilin-1 overexpression is frequently observed in tumors driving cancer progression through enhanced angiogenesis and tissue invasion closely interacting with proteins like VEGF-A. In cardiovascular disease Neuropilin-1 contributes to abnormal blood vessel formation and stability. By studying Neuropilin-1 and other biomarkers like CD304 FITC researchers aim to develop therapeutic strategies targeting its role in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins (PubMed : 10688880, PubMed : 9288753, PubMed : 9529250). Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage (PubMed : 19805273). It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB (PubMed : 10688880, PubMed : 19805273, PubMed : 9288753, PubMed : 9529250). Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR (PubMed : 30623799).. (Microbial infection) Acts as a host factor for human coronavirus SARS-CoV-2 infection. Recognizes and binds to CendR motif RRAR on SARS-CoV-2 spike protein S1 which enhances SARS-CoV-2 infection.. Isoform 2. Binds VEGF-165 and may inhibit its binding to cells (PubMed : 10748121, PubMed : 26503042). May induce apoptosis by sequestering VEGF-165 (PubMed : 10748121). May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
See full target information NRP1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 13:10809-10815 PubMed34650759

2021

Effect of thalidomide combined with TP chemotherapy on serum VEGF and NRP-1 levels advanced esophageal cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhong Zheng,Lijuan Fu

PloS one 14:e0222006 PubMed31490977

2019

Dextranol: An inert xeroprotectant.

Applications

Unspecified application

Species

Unspecified reactive species

Bryan J Jones,Advitiya Mahajan,Alptekin Aksan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

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AB213813

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