Human Neuroserpin ELISA Kit
- Recombinant
- SimpleStep
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(1 Publication)
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- sELISA
Supplier Data
Sandwich ELISA - Human Neuroserpin ELISA Kit (AB283539)
Interpolated concentrations of native Neuroserpin in human serum and plasma samples.
The concentrations of Neuroserpin were measured in duplicates, interpolated from the Neuroserpin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma-citrate 50%, plasma-heparin 50% and plasma-edta 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuroserpin concentration was determined to be 5464 pg/mL in serum, 5,291 pg/mL in plasma-citrate, 5,070 pg/mL in plasma-edta, and 5,265 pg/mL in plasma-heparin.
- sELISA
Supplier Data
Sandwich ELISA - Human Neuroserpin ELISA Kit (AB283539)
Serum from ten individual healthy human gender donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuroserpin concentration was determined to be 3.74 ng/mL with a range of 1.62 – 6.02 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human Neuroserpin ELISA Kit (AB283539)
Example of human Neuroserpin standard curve in 1X Cell Extraction Buffer PTR.
The Neuroserpin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Reactivity data
Product details
Human Neuroserpin ELISA kit (ab283539) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Neuroserpin protein in human serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Neuroserpin with 25 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Neuroserpin is a serine protease which plays a key role in neural growth regulation, protection from ischemic damage, learning, and behavior. It plays a key role in extra cellular matrix homeostasis through its regulatory role over the enzyme tissue-type plasminogen activator. Dysregulation of this protein is a hallmark of familial encephalopathy. The immunogen from which the antibodies in this kit were raised represent the full-length mature protein. This protein shares only 88% and 89% sequence identity with mouse and rat, respectively, and is not expected to be highly cross-reactive.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Neuroserpin plays an important role in nervous system development and maintenance. It inhibits tissue plasminogen activator (tPA) a protease involved in breaking down blood clots and controls extracellular matrix modifications. Neuroserpin does not form part of a complex but interacts with other key proteins for its function. Its regulation of tPA influences synaptic plasticity and protection of neurons from excessive proteolysis which is essential in maintaining proper brain function.
Pathways
Neuroserpin's activity is significant in the plasminogen activation system and neurotrophic pathways. It influences the balance of proteolytic activities that affect cell migration and synaptic remodeling. Related proteins in these pathways include proteases like tPA and neurotrophins like brain-derived neurotrophic factor (BDNF) which support neuronal survival.
Product protocols
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Publications (1)
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EJHaem 4:324-338 PubMed37206290
2023
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