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AB270204

Human Neutrophil Elastase ELISA Kit

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(6 Publications)

Human Neutrophil Elastase ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Neutrophil Elastase with a sensitivity of 15.8 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ELA2, ELANE, Neutrophil elastase, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, Medullasin, PMN elastase, HLE

10 Images
ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)
  • ELISA

Supplier Data

ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)
Sandwich ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)

Example of human Neutrophil Elastase standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.

The Neutrophil Elastase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)

Interpolated concentrations of native Neutrophil Elastase in human U937 cell extract based on a 2.5 μg/mL extract load.

The concentrations of Neutrophil Elastase were measured in duplicate and interpolated from the Neutrophil Elastase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)

Serum from eight individual healthy Neutrophil Elastase gender donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neutrophil Elastase concentration was determined to be 192 ng/mL with a range of 74 – 413 ng/mL.

Sandwich ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)

Interpolated concentrations of native Neutrophil Elastase in human serum, plasma (EDTA), plasma (citrate), plasma (heparin).

The concentrations of Neutrophil Elastase were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 100, plasma (EDTA) 1 : 50, Plasma (citrate) 1 : 100, and plasma (heparin)1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 225 ng/mL in serum, 113 ng/mL in plasma (EDTA), 215 ng/mL in plasma (citrate), and 291 ng/mL in plasma (heparin).

Sandwich ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)

Interpolated concentration of native Neutrophil Elastase was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 200 human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 25BS.

Sandwich ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)

Interpolated concentration of native Neutrophil Elastase was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2.5 µg/mL U937 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)

Example of human Neutrophil Elastase standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)

Example of human Neutrophil Elastase standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)

Example of human Neutrophil Elastase standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 15.8 pg/mL

Range

47 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Neutrophil Elastase ELISA Kit ab270204 is a rapid single-wash 90-min ELISA kit to measure Human Neutrophil Elastase cell culture media, cell Lysate, serum and plasma. 15.8 pg/mL.

How the assay works

Human Neutrophil Elastase SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and recombinant human Neutrophil Elastase protein in serum, plasmas, cell culture supernatant, and cell extract samples only. Urine, saliva, and milk samples have not been tested with this kit.

Human Neutrophil Elastase ELISA Kit ab270204 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human Neutrophil Elastase ELISA Kit ab270204

Human Neutrophil Elastase ELISA Kit ab270204 has been used to study the plasma proteome of long-covid patients(1) and to study interactions of neutrophil elastase with anti-thrombin in venous thromboembolism(2)

References:

(1) Cristiana Iosef et al. 2023, PMID: 37301958,
(2)Laura E Vincent et al. 2022, PMID: 35731524

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human Neutrophil Elastase Antibody Pair - BSA and Azide free ab253304
- Anti-Neutrophil Elastase antibody [EPR22896-31] - BSA and Azide free (Capture) ab272279
- Anti-Neutrophil Elastase antibody [EPR22896-78] - BSA and Azide free (Detector) ab272280

Related and recommended products

Human Neutrophil elastase is commonly used to study inflammation.

To measure Neutrophil elastase activity:

- Neutrophil Elastase Activity Assay Kit (Fluorometric) ab204730

Neutrophil Elastase, also known as bone marrow serine protease, Elastase-2, human leukocyte Elastase, and medullasin is a serine protease in the peptidase S1 family and is encoded by the ELANE gene. Neutrophil Elastase is expressed as a zymogen by neutrophils and macrophages during inflammation, where it breaks down bacterial and host membrane proteins. Neutrophil Elastase can localize to Neutrophil extracellular traps (NETs) by the protein’s affinity for DNA.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" } ]
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Recovery

[ { "sample": "Serum", "range": "105 - 120 %", "average": "= 112" }, { "sample": "EDTA Plasma", "range": "103 - 118 %", "average": "= 111" }, { "sample": "Cell culture media", "range": "103 - 109 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "105 - 113 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "100 - 105 %", "average": "= 102" }, { "sample": "Cell culture extracts", "range": "115 - 117 %", "average": "= 116" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neutrophil Elastase also known as neutrophil proteinase 3 has a molecular mass of approximately 30 kDa. It is a serine protease expressed mainly in azurophilic granules of neutrophils. This enzyme breaks down various proteins including elastin at sites of inflammation. Neutrophil Elastase releases during degranulation or through neutrophil extracellular traps (NETs) to combat pathogens.
Biological function summary

The target plays a significant role in neutrophil-mediated tissue remodeling and host defense. It leads to the breaking down of extracellular matrix components contributing to tissue remodeling and repair. Neutrophil Elastase is part of a complex interplay with other proteases like matrix metalloproteinases (MMPs). Its activity is regulated by endogenous inhibitors such as alpha-1 antitrypsin to prevent excessive tissue damage.

Pathways

This protease is important in inflammation and immune response pathways. It plays a role in the complement system by activating C5 a component of the complement cascade. It also acts in conjunction with other proteases such as cathepsin G to modulate immune and inflammatory responses. Neutrophil Elastase's role within these pathways is pivotal for the regulation of inflammation and destruction of pathogens.

Individuals with conditions such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis often exhibit dysregulation or excessive activity of Neutrophil Elastase. This excessive activity leads to tissue damage and exacerbates disease progression. The enzyme also interacts with proteins like elastin and collagen in these diseases further contributing to pathological tissue remodeling and damage.
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Product protocols

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Target data

Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed : 15140022). Promotes cleavage of GSDMB, thereby inhibiting pyroptosis (PubMed : 36899106). Promotes blood coagulation (PubMed : 20676107). Through the activation of the platelet fibrinogen receptor integrin alpha-IIb/beta-3, potentiates platelet aggregation induced by a threshold concentration of cathepsin G (CTSG) (PubMed : 25211214, PubMed : 9111081). Cleaves and thus inactivates tissue factor pathway inhibitor (TFPI) (PubMed : 20676107, PubMed : 25211214). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed : 10947984).
See full target information ELANE
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 15: PubMed40305335

2025

Metabolic Implications of Elevated Neutrophil Extracellular Traps in Polycystic Ovary Syndrome: A Focus on Hepatic Glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Siyu Lin,Yushan Li,Wei Liu,Yanzhi Du,Tao Tao

Scientific reports 15:8661 PubMed40082470

2025

Immature forms of low density granulocytes are increased in acute myeloid leukemia and myelodysplastic syndromes.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Bertolini,Francesca Picone,Idalucia Ferrara,Anna Maria Della Corte,Bianca Serio,Marisa Gorrese,Annapaola Campana,Vittorio Simeon,Serena Luponio,Rossella Marcucci,Pasqualina Scala,Maddalena Langella,Carmine Selleri,Valentina Giudice

Cancers 16: PubMed38398138

2024

Characterizing the Inflammatory Profile of Neutrophil-Rich Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fatma Al Qutami,Walaa AlHalabi,Aswathy Vijayakumar,Surendra Singh Rawat,Abubakr H Mossa,Manju Nidagodu Jayakumar,Baila Samreen,Mahmood Y Hachim

Journal of translational medicine 21:377 PubMed37301958

2023

Plasma proteome of Long-COVID patients indicates HIF-mediated vasculo-proliferative disease with impact on brain and heart function.

Applications

Unspecified application

Species

Unspecified reactive species

Cristiana Iosef,Michael J Knauer,Michael Nicholson,Logan R Van Nynatten,Gediminas Cepinskas,Sorin Draghici,Victor K M Han,Douglas D Fraser

JAMA surgery 157:713-721 PubMed35731524

2022

Association of Changes in Antithrombin Activity Over Time With Responsiveness to Enoxaparin Prophylaxis and Risk of Trauma-Related Venous Thromboembolism.

Applications

Unspecified application

Species

Unspecified reactive species

Laura E Vincent,Michael M Talanker,Dakota D Butler,Xu Zhang,Jeanette M Podbielski,Yao-Wei W Wang,Amber Chen-Goodspeed,Selina L Hernandez Gonzalez,Erin E Fox,Bryan A Cotton,Charles E Wade,Jessica C Cardenas

Critical care (London, England) 26:155 PubMed35637483

2022

Impairment of neutrophil functions and homeostasis in COVID-19 patients: association with disease severity.

Applications

Unspecified application

Species

Unspecified reactive species

Chloé Loyer,Arnaud Lapostolle,Tomas Urbina,Alexandre Elabbadi,Jean-Rémi Lavillegrand,Thomas Chaigneau,Coraly Simoes,Julien Dessajan,Cyrielle Desnos,Mélanie Morin-Brureau,Yannick Chantran,Pierre Aucouturier,Bertrand Guidet,Guillaume Voiriot,Hafid Ait-Oufella,Carole Elbim
View all publications
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