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AB317778

Human NF2/Merlin ELISA Kit

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Human NF2/Merlin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human NF2/Merlin with a sensitivity of 36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput

View Alternative Names

SCH, NF2, Merlin, Moesin-ezrin-radixin-like protein, Neurofibromin-2, Schwannomerlin, Schwannomin

4 Images
Sandwich ELISA - Human NF2/Merlin ELISA Kit (AB317778)
  • sELISA

Supplier Data

Sandwich ELISA - Human NF2/Merlin ELISA Kit (AB317778)

Example of human NF2/Merlin standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human NF2/Merlin ELISA Kit (AB317778)
  • sELISA

Supplier Data

Sandwich ELISA - Human NF2/Merlin ELISA Kit (AB317778)

Interpolated concentrations of human NF2/Merlin in MG-63 cell extract in 96-well vs. 384-well plates. Interpolated concentration of native NF2/Merlin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 62.5 ug/mL MG-63 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human NF2/Merlin ELISA Kit (AB317778)
  • sELISA

Supplier Data

Sandwich ELISA - Human NF2/Merlin ELISA Kit (AB317778)

Interpolated concentrations of human NF2/Merlin in MG-63 and U-2 OS cell extracts. Interpolated concentration of native NF2/Merlin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : MG-63 cell extract 62.5 ug/mL and U-2 OS cell extract 31.25 ug/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human NF2/Merlin ELISA Kit (AB317778)
  • sELISA

Supplier Data

Sandwich ELISA - Human NF2/Merlin ELISA Kit (AB317778)

Example of human NF2/Merlin standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 36 pg/mL

Range

156.25 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB109244

Anti-NF2 / Merlin antibody [EPR2573(2)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] (AB109244)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human NF2/Merlin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NF2/Merlin protein in Cell Lysate 85. Quantitate Human NF2/Merlin with 36 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "93 - 100 %", "average": "= 96" }, { "sample": "Tissue Extracts", "range": "92 - 99 %", "average": "= 97" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NF2 gene produces a protein known as Neurofibromin 2 often called Merlin. The NF2 protein or Merlin protein plays a mechanical role in the cell as a tumor suppressor by stabilizing cellular structure and regulating cell growth. Its molar mass is approximately 70 kDa. NF2 protein is found mainly in the nervous system particularly in Schwann cells which are important for nerve function.
Biological function summary

The NF2 protein functions to control cell proliferation differentiation and motility. It associates with the cytoskeleton acting as a bridge between cell surface proteins and the actin cytoskeleton. Merlin as part of the ERM (ezrin radixin moesin) family contributes to the assembly of protein complexes at the cell membrane. Through its interactions it plays a significant role in maintaining proper cell signaling and structural integrity.

Pathways

NF2 protein integrates into the Hippo signaling pathway which regulates organ size by controlling cell growth and apoptosis. Within this pathway Merlin interacts with members such as YAP1 and LATS1/2 moderating their activity to prevent over-proliferation of cells. NF2 also connects with the mTOR pathway which is critical for cell metabolism and growth further emphasizing its regulatory functions in cellular homeostasis.

Mutations or deletions in the NF2 gene are associated with neurofibromatosis type 2 a condition that causes benign tumors on nerve tissues known as schwannomas. NF2 protein is linked to other proteins such as angiogenesis factors contributing to the development of these tumors. Schwannomatosis another disorder related to NF2 involves nerve sheath tumor formation with Merlin mutation playing a role indicating its importance in nervous system health.
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Product protocols

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Target data

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.
See full target information NF2
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websiteProtocolBooklet
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Product promise

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