AB277704

Human NFAT2 ELISA Kit (NFATC1)

Recombinant
SimpleStep
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Human NFAT2 ELISA Kit (NFATC1) is a single-wash 90-min Simplestep used to quantify Human NFAT2 (NFATC1) with a sensitivity of 4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

NFAT2, NFATC, NFATC1, NF-ATc1, NFATc1, NFAT transcription complex cytosolic component, NF-ATc, NFATc

2 Images

Sandwich ELISA - Human NFAT2 ELISA Kit (NFATC1) (AB277704)

sELISA

Supplier Data

Sandwich ELISA - Human NFAT2 ELISA Kit (NFATC1) (AB277704)

Interpolated concentrations of native NFAT2 in human RAMOS Cell Extract based on a 1,000 μg/mL extract load.

The concentrations of NFAT2 were measured in duplicate and interpolated from the NFAT2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NFAT2 concentration was determined to be 779 pg/mL.

sELISA

Unknown

Sandwich ELISA - Human NFAT2 ELISA Kit (NFATC1) (AB277704)

Example of human NFAT2 standard curve in 1X Cell Extraction Buffer PTR.

The NFAT2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 4 pg/mL

Range

19.53 - 1250 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human NFAT2 ELISA kit (ab277704) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human NFAT2 protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human NFAT2 with 4 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Nuclear factor of activated T-cells, cytoplasmic 1, also known as NFATC1 or NFAT2, is a transcription factor that plays a key role in regulating the immune response. With its complex partners, NFAT2 regulates the expression of cytokines and differentiation of T-lymphocytes. This protein is upregulated in certain type of cancers including chronic lymphocytic leukemia. The immunogen with which the antibodies for this kit were raised share high sequence identity with mouse and rat, and are therefore, expected to cross-react.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.87" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "80 - 96 %", "average": "= 91" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277704-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB277704-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB277704-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB277704-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB277704-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB277704-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human NFAT2 (NFATC1) Capture Antibody", "number":"AB277704-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB277704-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human NFAT2 (NFATC1) Lyophilized Recombinant Protein", "number":"AB277704-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB277704-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human NFAT2 (NFATC1) Detector Antibody", "number":"AB277704-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB277704-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NFAT2 also known as NFATc1 is a transcription factor with a mass of approximately 100 kDa. It belongs to the Nuclear Factor of Activated T-cells (NFAT) family which plays significant roles in gene expression regulation. NFAT2 is expressed in T-cells B-cells and cardiac muscle tissues. It is activated through dephosphorylation by the enzyme calcineurin allowing it to translocate into the nucleus. NFAT2 binds to specific DNA motifs to regulate the transcription of target genes impacting various cellular processes.

Biological function summary

NFAT2 plays a significant role in the regulation of immune responses and cardiac hypertrophy. It functions as part of a complex with AP-1 (Activator Protein-1) during T-cell activation integrating signals necessary for cytokine production and immune cell differentiation. In cardiac cells NFAT2 influences the expression of genes involved in hypertrophic growth. NFAT2 contributes to the development and function of the immune system by modulating critical immune pathways and cellular proliferation.

Pathways

NFAT2 serves a vital function in the calcineurin-NFAT signaling pathway. This pathway is important for T-cell activation and is triggered by increased intracellular calcium levels leading to dephosphorylation of NFAT2. The dephosphorylated NFAT2 cooperates with proteins such as AP-1 to induce expression of cytokines like interleukin-2. NFAT2 is also linked with the MAPK signaling pathway which affects cellular proliferation and differentiation. Together these pathways highlight the integral role of NFAT2 in immune system function and cardiac development.

NFAT2 has strong connections to autoimmune diseases and cardiac hypertrophy. Aberrations in NFAT2 activity and regulation are associated with autoimmune conditions due to its central role in immune response modulation. In cardiac hypertrophy heightened NFAT2 activity may lead to pathological enlargement of heart tissue. The protein has interactions with calcineurin which also influences these conditions. Understanding these relationships helps in exploring therapeutic targets for managing immune-related diseases and cardiac dysfunctions.

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Product protocols

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Target data

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells (PubMed : 10358178). Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function (By similarity).

See full target information NFATC1

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

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Human NFAT2 ELISA Kit (NFATC1)

Sandwich ELISA - Human NFAT2 ELISA Kit (NFATC1) (AB277704)

Sandwich ELISA - Human NFAT2 ELISA Kit (NFATC1) (AB277704)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

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Product promise