AB322433

Human NFAT4/NF-ATc3 ELISA Kit

Recombinant
SimpleStep
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Human NFAT4/NF-ATc3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human NFAT4/NF-ATc3 with a sensitivity of 11.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

NFAT4, NFATC3, NF-ATc3, NFATc3, NFATx, T-cell transcription factor NFAT4, NF-AT4, NF-AT4c

4 Images

Sandwich ELISA - Human NFAT4/NF-ATc3 ELISA Kit (AB322433)

sELISA

Supplier Data

Sandwich ELISA - Human NFAT4/NF-ATc3 ELISA Kit (AB322433)

Interpolated concentration of native NFAT4/NF-ATc3 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 300 µg/mL human tonsil tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human NFAT4/NF-ATc3 ELISA Kit (AB322433)

Interpolated concentration of native NFAT4/NF-ATc3 was measured in duplicate at different sample concentrations. Undiluted samples are 300 µg/mL human tonsil tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human NFAT4/NF-ATc3 ELISA Kit (AB322433)

Example of human NFAT4/NF-ATc3 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human NFAT4/NF-ATc3 ELISA Kit (AB322433)

Example of human NFAT4/NF-ATc3 standard curve. Background-subtracted data values (mean +/- SD) are graphed

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 11.3 pg/mL

Range

28.125 - 1800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human NFAT4/NF-ATc3 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NFAT4/NF-ATc3 protein in Tissue extracts. Quantitate Human NFAT4/NF-ATc3 with 11.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": "94 - 107 %", "average": "= 101" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Acts as a regulator of transcriptional activation. Binds to the TNFSF11/RANKL promoter region and promotes TNFSF11 transcription (By similarity). Binding to the TNFSF11 promoter region is increased by high levels of Ca(2+) which induce NFATC3 expression and may lead to regulation of TNFSF11 expression in osteoblasts (By similarity). Plays a role in promoting mesenteric arterial wall remodeling in response to the intermittent hypoxia-induced increase in EDN1 and ROCK signaling (By similarity). As a result NFATC3 colocalizes with F-actin filaments, translocates to the nucleus and promotes transcription of the smooth muscle hypertrophy and differentiation marker ACTA2 (By similarity). Promotes lipopolysaccharide-induced apoptosis and hypertrophy in cardiomyocytes (By similarity). Following JAK/STAT signaling activation and as part of a complex with NFATC4 and STAT3, binds to the alpha-beta E4 promoter region of CRYAB and activates transcription in cardiomyocytes (By similarity). In conjunction with NFATC4, involved in embryonic heart development via maintenance of cardiomyocyte survival, proliferation and differentiation (By similarity). Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 (PubMed : 18815128). Required for thymocyte maturation during DN3 to DN4 transition and during positive selection (By similarity). Positively regulates macrophage-derived polymicrobial clearance, via binding to the promoter region and promoting transcription of NOS2 resulting in subsequent generation of nitric oxide (By similarity). Involved in Ca(2+)-mediated transcriptional responses upon Ca(2+) influx via ORAI1 CRAC channels.

See full target information NFATC3

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websiteProtocolBooklet

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Product promise

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