AB323741

Human NFATC4 ELISA Kit

Recombinant
SimpleStep
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Human NFATC4 ELISA Kit is a Sandwich ELISA for the measurement of Human NFATC4 in Human in Cell/Tissue Extracts samples.

View Alternative Names

NFAT3, NFATC4, NF-ATc4, NFATc4, T-cell transcription factor NFAT3, NF-AT3

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Sandwich ELISA - Human NFATC4 ELISA Kit (AB323741)

sELISA

Supplier Data

Sandwich ELISA - Human NFATC4 ELISA Kit (AB323741)

Interpolated concentration of native NFATC4 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2.0 mg/ human lung tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Supplier Data

Sandwich ELISA - Human NFATC4 ELISA Kit (AB323741)

Example of human NFATC4 standard curve in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human NFATC4 ELISA Kit (AB323741)

Example of human NFATC4 standard curve in 1X Cell Extraction Buffer SSW in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human NFATC4 ELISA Kit (AB323741)

Interpolated concentration of native NFATC4 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HeLa cell extract 1.0 mg/mL and human lung tissue extract 1.0 mg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 27.908 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human NFATC4 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NFATC4 protein in Cell Lysate, Tissue extracts. Quantitate Human NFATC4 with 27.908 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.1" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 10.9" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "95 - 110 %", "average": "= 100" }, { "sample": "Tissue Extracts", "range": "81 - 85 %", "average": "= 84" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Ca(2+)-regulated transcription factor that is involved in several processes, including the development and function of the immune, cardiovascular, musculoskeletal, and nervous systems (PubMed : 11514544, PubMed : 11997522, PubMed : 17213202, PubMed : 17875713, PubMed : 18668201, PubMed : 25663301, PubMed : 7749981). Involved in T-cell activation, stimulating the transcription of cytokine genes, including that of IL2 and IL4 (PubMed : 18347059, PubMed : 18668201, PubMed : 7749981). Along with NFATC3, involved in embryonic heart development. Following JAK/STAT signaling activation and as part of a complex with NFATC3 and STAT3, binds to the alpha-beta E4 promoter region of CRYAB and activates transcription in cardiomyocytes (By similarity). Involved in mitochondrial energy metabolism required for cardiac morphogenesis and function (By similarity). Transactivates many genes involved in the cardiovascular system, including AGTR2, NPPB/BNP (in synergy with GATA4), NPPA/ANP/ANF and MYH7/beta-MHC (By similarity). Involved in the regulation of adult hippocampal neurogenesis. Involved in BDNF-driven pro-survival signaling in hippocampal adult-born neurons. Involved in the formation of long-term spatial memory and long-term potentiation (By similarity). In cochlear nucleus neurons, may play a role in deafferentation-induced apoptosis during the developmental critical period, when auditory neurons depend on afferent input for survival (By similarity). Binds to and activates the BACE1/Beta-secretase 1 promoter, hence may regulate the proteolytic processing of the amyloid precursor protein (APP) (PubMed : 25663301). Plays a role in adipocyte differentiation (PubMed : 11997522). May be involved in myoblast differentiation into myotubes (PubMed : 17213202). Binds the consensus DNA sequence 5'-GGAAAAT-3' (Probable). In the presence of CREBBP, activates TNF transcription (PubMed : 11514544). Binds to PPARG gene promoter and regulates its activity (PubMed : 11997522). Binds to PPARG and REG3G gene promoters (By similarity).

See full target information NFATC4

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Product promise

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