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AB155436

Human NGF R ELISA Kit (CD271)

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(1 Publication)

Human NGF R ELISA Kit (CD271) is a Sandwich (quantitative) ELISA for the measurement of Human NGF R (CD271) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD271, TNFRSF16, NGFR, Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR, p75 ICD, NGF receptor

1 Images
Sandwich ELISA - Human NGF R ELISA Kit (CD271) (AB155436)
  • sELISA

Unknown

Sandwich ELISA - Human NGF R ELISA Kit (CD271) (AB155436)

NGF R (CD271) Human ELISA kit.

Representative standard curve using ab155436

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 80 pg/mL

Range

0.082 - 20 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's NGF R (CD271) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human NGF R (CD271) in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human NGF R coated on a 96-well plate. Standards and samples are pipetted into the wells and NGF R present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human NGF R antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NGF R bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

The standard is affinity purified.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "79 - 95 %", "average": "= 86.88" }, { "sample": "Serum", "range": "75 - 93 %", "average": "= 82.75" }, { "sample": "Plasma", "range": "76 - 93 %", "average": "= 84.17" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155436-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155436-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155436-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"NGF R Microplate (12 x 8 wells)", "number":"AB155436-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human NGF R Standard (lyophilized)", "number":"AB155436-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155436-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155436-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB155436-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB155436-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human NGF R", "number":"AB155436-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Low affinity receptor which can bind to NGF, BDNF, NTF3, and NTF4. Forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF, BDNF and NTF3 with high affinity, and has much lower affinity for mature NGF and BDNF (PubMed : 24908487). Plays an important role in differentiation and survival of specific neuronal populations during development (By similarity). Can mediate cell survival as well as cell death of neural cells. Plays a role in the inactivation of RHOA (PubMed : 26646181). Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Necessary for the circadian oscillation of the clock genes BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCmgetaN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver (PubMed : 23785138).
See full target information NGFR
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational psychiatry 7:e1079 PubMed28375203

2017

The serum protein levels of the tPA-BDNF pathway are implicated in depression and antidepressant treatment.

Applications

ELISA

Species

Unspecified reactive species

H Jiang,S Chen,C Li,N Lu,Y Yue,Y Yin,Y Zhang,X Zhi,D Zhang,Y Yuan
View all publications
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