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AB313906

Human Nidogen 2/NID-2 ELISA Kit

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Human Nidogen 2/NID-2 ELISA Kit is a Sandwich ELISA for the measurement of Human Nidogen 2/NID-2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Nidogen-2, NID-2, Osteonidogen, NID2

1 Images
Sandwich ELISA - Human Nidogen 2/NID-2 ELISA Kit (AB313906)
  • sELISA

Supplier Data

Sandwich ELISA - Human Nidogen 2/NID-2 ELISA Kit (AB313906)

Example of standard curve using ab313906. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 49 pg/mL

Range

49 - 12000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB131279

Anti-Nidogen 2/NID-2 antibody [EPR8697]

RabMAb|Recombinant|Trial Size

Western blot - Anti-Nidogen 2/NID-2 antibody [EPR8697] (AB131279)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Nidogen 2/NID-2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Nidogen 2/NID-2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Nidogen 2/NID-2 coated on a 96- well plate.

Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ACE, ALK-4, ADAM23, Artemin, CT-1, Cathepsin V, FABP1, FGF-20, GDF-8, HAI-1, IL-27 R alpha (WSX-1), Insulin R (CD220), Kallikrein 7, LIF R alpha, Lipocalin-1, LTbR (Lymphotoxin beta R), Mesothelin, MFRP, Neuropilin-2, Neurturin, Olfactomedin-2 (Noelin-2), p53, PDECGF, PDGF-CC, Progranulin, Ret, ROBO4, Semaphorin 6B, Serpin F1 (PEDF), SREC-I (SCARF1), SREC-II (SCARF2), TLR1, TLR3, TPP1, TREM-2, TrkC, TROY, Uromodulin, XIAP.

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Recovery

[ { "sample": "Serum", "range": "88 - 145 %", "average": "= 119.9" }, { "sample": "Plasma", "range": "75 - 147 %", "average": "= 110" }, { "sample": "Cell culture media", "range": "72 - 124 %", "average": "= 95.79" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313906-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Human Nidogen 2/NID-2 Antibody-coated ELISA Plate", "number":"AB313906-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313906-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Nidogen 2/NID-2 Detection Antibody", "number":"AB313906-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313906-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313906-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB313906-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Nidogen 2/NID-2 Protein Standard", "number":"AB313906-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313906-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'Nidogen 2' also called 'NID-2' or '"nidogen protein'" or '"nid-2"' is a glycoprotein found in basement membranes. It has a molecular mass around 150 kDa and expresses across various tissues particularly in the vascular structures and certain epithelial tissues. This protein has sulfated and carbohydrate-rich regions making it important in forming stable extracellular matrix architecture.
Biological function summary

'nidogen 2' plays roles in maintaining the structural integrity of basement membranes. It interacts with other matrix components like laminin and collagen IV forming part of complexes that ensure the basal membrane's maintenance and stability. 'NID-2' provides structural support and helps secure cellular environments which is important for tissue repair and morphogenesis.

Pathways

'nidogen 2' contributes to the extracellular matrix remodeling and cell signaling pathways. It participates in the integrin signaling pathway where it interacts with proteins such as 'integrin' and 'perlecan'. These interactions help mediate cellular adhesion and signal transduction impacting cellular behavior like proliferation differentiation and migration processes.

Abnormalities in 'nidogen 2' relate to certain cancers and kidney disorders. In cancer disrupted interactions with 'laminin' may promote tumor cell invasion and metastasis. In kidney disorders such as nephrotic syndrome altered 'nidogen 2' function relates to basement membrane defects affecting kidney filtration function. Understanding its role could lead to insights into therapies for these conditions.
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Product protocols

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Target data

Cell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions.
See full target information Nidogen-2
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websiteProtocolBooklet
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Product promise

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