JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB313912

Human Niemann Pick C1 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Niemann Pick C1 ELISA Kit is a Sandwich ELISA for the measurement of Human Niemann Pick C1 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

NPC intracellular cholesterol transporter 1, Niemann-Pick C1 protein, NPC1

1 Images
Sandwich ELISA - Human Niemann Pick C1 ELISA Kit (AB313912)
  • sELISA

Supplier Data

Sandwich ELISA - Human Niemann Pick C1 ELISA Kit (AB313912)

Example of standard curve using ab313912. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.12 ng/mL

Range

0.12 - 30 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB134113

Anti-Niemann Pick C1 antibody [EPR5209]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Niemann Pick C1 antibody [EPR5209] (AB134113)

  • 5
  • 2
Proteins & Peptides

AB276725

Recombinant Human Niemann Pick C1 protein (His tag)

SDS-PAGE - Recombinant Human Niemann Pick C1 protein (His tag) (AB276725)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Niemann Pick C1 (NPC intracellular cholesterol transporter 1) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Niemann Pick C1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Niemann Pick C1 coated on a 96-well plate.
style
left-column

Recovery

[ { "sample": "Serum", "range": "70 - 122 %", "average": "= 97.08" }, { "sample": "Plasma", "range": "77 - 113 %", "average": "= 96.49" }, { "sample": "Cell culture media", "range": "96 - 124 %", "average": "= 114.5" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313912-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313912-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313912-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Niemann Pick C1 Antibody-coated ELISA Plate", "number":"AB313912-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313912-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin", "number":"AB313912-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Niemann Pick C1 Detection Antibody", "number":"AB313912-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313912-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Niemann Pick C1 Protein Standard", "number":"AB313912-CMP10", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Niemann Pick C1 also known as NPC1 or C1 protein is an integral membrane protein involved in cholesterol and lipid transport within cells. It is located in late endosomes and lysosomes and plays a role in intracellular cholesterol traffic. The NPC1 protein has a molecular weight of approximately 170 kDa. It is expressed in various tissues with high expression levels in the liver brain and spleen.
Biological function summary

This protein acts in the movement of lipids and cholesterol through cellular compartments. NPC1 functions as part of a protein complex that includes NPC2 which works to mobilize cholesterol from lysosomes to other areas in the cell where it is further processed or stored. This transport mechanism is necessary to maintain cellular lipid homeostasis and proper cellular functions.

Pathways

NPC1 integrates into the cholesterol trafficking pathway and plays an essential part in intracellular lipid regulation. It interacts with proteins involved in cholesterol metabolism such as sterol regulatory element-binding proteins (SREBPs). These interactions ensure that cholesterol distribution within the cell remains balanced and adjusts to metabolic needs. The proper function of NPC1 in these pathways is necessary for maintaining cell health and homeostasis.

NPC1 mutations are directly linked to Niemann-Pick disease type C a severe lipid storage disorder. This condition is characterized by the accumulation of cholesterol and other lipids in lysosomes leading to neurological and hepatic dysfunction. NPC1's role in this disease involves disrupted cholesterol trafficking which is critical for cellular lipid homeostasis. Additionally NPC2 protein is also affected in this disorder as both work together in the cholesterol transport process.
style
left-column

Product protocols

style
left-column

Target data

Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment (PubMed : 10821832, PubMed : 12554680, PubMed : 18772377, PubMed : 27238017, PubMed : 9211849, PubMed : 9927649). Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1 (PubMed : 18772377, PubMed : 19563754, PubMed : 27238017, PubMed : 27378690, PubMed : 28784760, PubMed : 9211849, PubMed : 9927649). Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket (PubMed : 19563754). Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals (Probable). Inhibits cholesterol-mediated mTORC1 activation throught its interaction with SLC38A9 (PubMed : 28336668).. (Microbial infection) Acts as an endosomal entry receptor for ebolavirus.
See full target information NPC intracellular cholesterol transporter 1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com