Human Niemann Pick C2 ELISA Kit
- Recombinant
- SimpleStep
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Human Niemann Pick C2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Niemann Pick C2 with a sensitivity of 2.58 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
HE1, NPC2, NPC intracellular cholesterol transporter 2, Epididymal secretory protein E1, Human epididymis-specific protein 1, Niemann-Pick disease type C2 protein, He1
- sELISA
Supplier Data
Sandwich ELISA - Human Niemann Pick C2 ELISA Kit (AB227894)
Example of Human Niemann Pick C2 standard curve Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Niemann Pick C2 ELISA Kit (AB227894)
Interpolated concentrations of native Niemann Pick C2 in Human cell and tissue extract samples.
The concentrations of Niemann Pick C2 were measured in duplicate, interpolated from the Niemann Pick C2 standard curves and corrected for sample dilution. Undiluted samples are as follows : HepG2 cell extract (100 mg/ml), Human Pancreas (100 mg/ml) and Human Placenta (100 mg/ml) extracts. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Niemann Pick C2 concentration was determined to be 286.36 pg/mL in HepG2 extract, 345.68 pg/mL in Human Pancreas extract and 303.6 pg/mL in Human Placenta extract based on the 100 μg/mL load.
- sELISA
Supplier Data
Sandwich ELISA - Human Niemann Pick C2 ELISA Kit (AB227894)
Example of Human Niemann Pick C2 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Niemann Pick C2 ELISA Kit (AB227894)
Interpolated concentrations of native Niemann Pick C2 in Human HepG2 Supernatant (2.5%).
The concentrations of Niemann Pick C2 were measured in duplicate and interpolated from the Niemann Pick C2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Niemann Pick C2 concentration was determined to be 3288.10 pg/mL in HepG2 Supernatant.
Reactivity data
Product details
Human Niemann Pick C2 ELISA Kit (ab227894) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Niemann Pick C2 protein in cell culture extracts, cell culture supernatant, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Niemann Pick C2 with 2.58 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NPC2 protein facilitates the transfer of cholesterol from lysosomes to other parts of the cell. It operates in tandem with its partner protein Niemann Pick C1 (NPC1) to mediate the egress of cholesterol. The NPC2 protein docks to NPC1 within the lysosomal membrane forming an essential complex in lipid transport. This function is pivotal for the maintenance of cellular cholesterol homeostasis influencing various cellular and systemic functions linked to cholesterol metabolism.
Pathways
NPC2 protein participates prominently in the cholesterol efflux pathways. It associates with the NPC1 protein to regulate the movement of cholesterol from the lysosomal membrane to cytoplasmic areas where it can be utilized or stored. This pathway links NPC2 to significant biological processes like the homeostasis of lipoprotein-derived cholesterol influencing the SREBP pathway which governs lipid synthesis and uptake. Such interactions highlight NPC2's involvement in regulating cellular lipid balance.
Product protocols
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- Download websiteProtocolBooklet|en
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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