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AB313907

Human NKp30 ELISA Kit

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Human NKp30 ELISA Kit is a Sandwich ELISA for the measurement of Human NKp30 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

CD337, 1C7, LY117, NCR3, Natural cytotoxicity triggering receptor 3, Activating natural killer receptor p30, Natural killer cell p30-related protein, NK-p30, NKp30

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Sandwich ELISA - Human NKp30 ELISA Kit (AB313907)
  • sELISA

Supplier Data

Sandwich ELISA - Human NKp30 ELISA Kit (AB313907)

Example of standard curve using ab313907. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.085 ng/mL

Range

0.085 - 20 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human NKp30 (also known as NCR3 ) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human NKp30 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human NKp30 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ALK-1, B7-H2, BLAME, BMP-8, CD28, Common beta Chain (CD131), Contactin-1, Desmoglein-1, Desmoglein-3, EDAR, EphA1, EphB6, Ephrin-B3, Epiregulin, FGF-12, FGF-17, FOLR2, Galectin-8, Glypican 1, Glypican 5, GHR, IFNgamma R1, IL-22 R alpha 1, IL-22BP, IL-23 R, IL-31 RA, IL-7 R alpha, Integrin alpha 5, MDM2, Nectin-1, Nogo Receptor, Notch-3, OSM R beta, Prolactin R, RELT, Ryk, Semaphorin 6D, Semaphorin 7A, Siglec-11.

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Recovery

[ { "sample": "Serum", "range": "95 - 111 %", "average": "= 102.7" }, { "sample": "Plasma", "range": "89 - 111 %", "average": "= 98.86" }, { "sample": "Cell culture media", "range": "96 - 122 %", "average": "= 110.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313907-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313907-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313907-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human NKp30 Antibody-coated ELISA Plate", "number":"AB313907-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB313907-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313907-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human NKp30 Protein Standard", "number":"AB313907-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human NKp30 Detection Antibody", "number":"AB313907-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB313907-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313907-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NKp30 also known as NCR3 (Natural Cytotoxicity Receptor 3) is a type I transmembrane protein that functions as an activating receptor on natural killer (NK) cells. It is part of the natural cytotoxicity receptor family and is encoded by the NCR3 gene. NKp30 is characterized by its structure which includes a single immunoglobulin-like domain. This protein weighs approximately 30 kDa. NKp30 is expressed mainly on resting and activated NK cells as well as IL-15-stimulated NK cells found in humans.
Biological function summary

NKp30 plays a significant role in immune response by recognizing and binding to ligands on tumor cells and virus-infected cells facilitating their elimination. It forms part of a larger immunological complex that includes DNAM-1 and 2B4 other activating receptors that work in concert to enhance the natural killing ability of NK cells. NKp30 triggers the release of cytotoxic granules and cytokines like IFN-γ leading to an enhanced cytotoxic response against target cells.

Pathways

NKp30 participates heavily in immune regulation especially in NK cell-mediated cytotoxicity. This protein is involved in pathways such as the NK cell-mediated antibody-dependent cellular cytotoxicity (ADCC) and has strong interaction with signaling proteins like CD3ζ and DAP12 which transduce activating signals. Through these pathways NKp30 influences the immune surveillance capability of NK cells modulating immune responses and maintaining cellular homeostasis.

NKp30 has been implicated in cancer progression and immune evasion notably in melanoma and gastrointestinal stromal tumors (GIST). Altered expression of NKp30 can result in reduced immune recognition and elimination of these tumor cells. The protein's interaction with B7-H6 a ligand often overexpressed on cancer cells illustrates its importance in tumor immunity. NKp30's function or dysfunction makes it a relevant target for therapeutic interventions aimed at restoring immune competence against cancerous cells.
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Product protocols

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Target data

Cell membrane receptor of natural killer/NK cells that is activated by binding of extracellular ligands including BAG6 and NCR3LG1. Stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells. Engagement of NCR3 by BAG6 also promotes myeloid dendritic cells (DC) maturation, both through killing DCs that did not acquire a mature phenotype, and inducing the release by NK cells of TNFA and IFNG which promote DC maturation.
See full target information NCR3
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websiteProtocolBooklet
en
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Product promise

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