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AB313909

Human Nmnat1/NMNAT ELISA Kit

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Human Nmnat1/NMNAT ELISA Kit is a Sandwich ELISA for the measurement of Human Nmnat1/NMNAT in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

NMNAT, NMNAT1, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1, NMN/NaMN adenylyltransferase 1, Nicotinamide-nucleotide adenylyltransferase 1, Nicotinate-nucleotide adenylyltransferase 1, NMN adenylyltransferase 1, NaMN adenylyltransferase 1

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Sandwich ELISA - Human Nmnat1/NMNAT ELISA Kit (AB313909)
  • sELISA

Supplier Data

Sandwich ELISA - Human Nmnat1/NMNAT ELISA Kit (AB313909)

Example of standard curve using ab313909. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 8 pg/mL

Range

8.19 - 2000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB118270

Anti-Nmnat1/NMNAT antibody [OTI1F7]

Immunocytochemistry/ Immunofluorescence - Anti-Nmnat1/NMNAT antibody [OTI1F7] (AB118270)

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Proteins & Peptides

AB198473

Recombinant human Nmnat1/NMNAT protein (His tag N-Terminus)

Functional Studies - Recombinant human Nmnat1/NMNAT protein (His tag N-Terminus) (AB198473)

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Assay Kits

AB221820

NMNAT1 Activity Assay Kit (Colorimetric)

Functional Studies - NMNAT1 Activity Assay Kit (Colorimetric) (AB221820)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human NMNAT-1 (Nicotinamide mononucleotide adenylyltransferase-1) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human NMNAT-1 in serum, plasma (collect plasma using EDTA and Citrate as an anticoagulant. Heparin is not recommended), and cell culture supernatants. This assay employs an antibody specific for human NMNAT-1 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "100 - 113 %", "average": "= 104.4" }, { "sample": "Plasma", "range": "79 - 99 %", "average": "= 88.9" }, { "sample": "Cell culture media", "range": "76 - 83 %", "average": "= 78.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313909-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Lyophilized Human Nmnat1/NMNAT Protein Standard", "number":"AB313909-CMP11", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313909-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Nmnat1/NMNAT Detection Antibody", "number":"AB313909-CMP10", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB313909-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313909-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313909-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB313909-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Nmnat1/NMNAT Antibody-coated ELISA Plate", "number":"AB313909-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313909-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nmnat1 also known as nicotinamide mononucleotide adenylyltransferase 1 is an enzyme involved in the conversion of nicotinamide mononucleotide (NMN) to nicotinamide adenine dinucleotide (NAD+). This enzyme weighs approximately 32 kDa and is expressed largely in mammalian tissues with higher concentrations in the brain heart and liver. In the research community variations like 'nmn ireland' or 'elisa namn' may refer to the study tools or location of research related to NMNAT.
Biological function summary

NMNAT1 plays a role in NAD+ biosynthesis which is essential for energy metabolism and cellular functions. It acts as a NAD+ synthase and may not work alone but within larger enzymatic complexes that handle cellular redox reactions. In neurons the enzyme supports axonal survival which connects it to neurodegenerative health studies.

Pathways

This enzyme becomes critical in the NAD+ biosynthesis pathway interacting closely with proteins such as nicotinamide phosphoribosyltransferase (NAMPT) in the salvage pathway. This pathway is key in maintaining cellular energy balance and gene expression regulation. The enzyme along with these related proteins ensures the recycling and regulation of NAD+ within the cells.

NMNAT1 holds significance in conditions like axonopathy and certain retinal degenerations. Mutations in the NMNAT1 gene have been linked to Leber's congenital amaurosis a severe genetic eye disorder. Studies often focus on NMNAT1 alongside other proteins like sirtuins as these participate in mitigating the oxidative stress aspects involved in neurodegeneration and associated disorders.
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Product protocols

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Target data

Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP (PubMed : 17402747). Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency (PubMed : 17402747). Can use triazofurin monophosphate (TrMP) as substrate (PubMed : 17402747). Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+) (PubMed : 17402747). For the pyrophosphorolytic activity, prefers NAD(+) and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively (PubMed : 17402747). Involved in the synthesis of ATP in the nucleus, together with PARP1, PARG and NUDT5 (PubMed : 27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed : 27257257). Also acts as a cofactor for glutamate and aspartate ADP-ribosylation by directing PARP1 catalytic activity to glutamate and aspartate residues on histones (By similarity). Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+) (PubMed : 17402747). Protects against axonal degeneration following mechanical or toxic insults (By similarity). Neural protection does not correlate with cellular NAD(+) levels but may still require enzyme activity (By similarity).
See full target information NMNAT1
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Product promise

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