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AB256391

Human Nodal ELISA Kit

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Human Nodal ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Nodal with a sensitivity of 0.32 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Nodal homolog, NODAL

2 Images
Sandwich ELISA - Human Nodal ELISA Kit (AB256391)
  • sELISA

Supplier Data

Sandwich ELISA - Human Nodal ELISA Kit (AB256391)

Interpolated concentrations of native Nodal in mouse cell culture supernatant samples.

The concentrations of Nodal were measured in duplicates, interpolated from the Nodal standard curves and corrected for sample dilution. Undiluted samples are as follows : F9 cell supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nodal concentration was determined to be 9.7 ng/mL in F9 supernatant.

Sandwich ELISA - Human Nodal ELISA Kit (AB256391)
  • sELISA

Supplier Data

Sandwich ELISA - Human Nodal ELISA Kit (AB256391)

Example of human Nodal standard curve in Sample Diluent NS.

The Nodal standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.32 ng/mL

Range

0.781 - 50 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Nodal ELISA Kit (ab256391) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Nodal protein in cell culture media and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Nodal with 0.32 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Human Nodal homolog is a secreted member of the TGF-beta superfamily of proteins encoded by the gene *NODAL*. Nodal binds to Type I and II serine/threonine kinase receptors, which recruits members of the SMAD family of transcription factors. Nodal signaling regulates mesoderm formation and axial patterning during embryonic development.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.9" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.4" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "86 - 91 %", "average": "= 88" }, { "sample": "Cell culture media", "range": "95 - 99 %", "average": "= 97" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab256391-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB256391-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB256391-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB256391-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB256391-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB256391-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB256391-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Nodal Capture Antibody", "number":"AB256391-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Nodal Lyophilized Recombinant Protein", "number":"AB256391-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"10X Human Nodal Detector Antibody", "number":"AB256391-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB256391-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nodal also known as NODAL protein is a growth factor belonging to the transforming growth factor beta (TGF-β) superfamily with a molecular mass of approximately 44 kDa. This protein acts mechanically by transmitting signals through receptors on the cell surface initiating intracellular responses critical for embryonic development. Nodal expression occurs mostly in early embryonic tissues including the primitive streak and visceral endoderm. In adults it shows limited expression yet you might find it in some cancer cells. Nodal antibodies can be used for research to study its expression patterns and roles in various contexts.
Biological function summary

Nodal plays an important role in processes such as mesoderm formation and left-right body axis specification. It is part of a signaling complex that includes co-factors like Cripto and receptor heteromers composed of Activin receptors and SMAD proteins. These interactions initiate cascades influencing cell fate decisions impacting diverse biological activities like cell proliferation and migration. Antinodal mechanisms act to modulate these signals ensuring proper gradient formation necessary for precise developmental outcomes.

Pathways

Nodal integrally fits within the TGF-β signaling pathway and the Wnt signaling pathway. It closely interacts with proteins such as Lefty and PITX2. The TGF-β pathway involves multiple SMAD proteins directing Nodal signals toward gene expression changes important for embryonic patterning. Meanwhile interactions with the Wnt pathway provide cross-talk opportunities that can affect a cell's destiny highlighting Nodal's broad regulatory capacity in embryogenesis.

Nodal is implicated in conditions like cancer and congenital abnormalities. In cancer aberrant Nodal expression connects with increased tumor aggressiveness and metastasis often through pathways involving proteins such as E-cadherin and N-cadherin which are important for cell adhesion and motility. In congenital disorders mutations or dysregulation in Nodal signaling can result in defects like holoprosencephaly often tied to the Hedgehog signaling pathway illustrating its critical developmental contributions.
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Product protocols

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Target data

Essential for mesoderm formation and axial patterning during embryonic development.
See full target information Nodal homolog
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websiteProtocolBooklet
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