AB283879

Human Noggin ELISA Kit

Recombinant
SimpleStep
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Human Noggin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Noggin with a sensitivity of 11 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Noggin, NOG

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Sandwich ELISA - Human Noggin ELISA Kit (AB283879)

sELISA

Supplier Data

Sandwich ELISA - Human Noggin ELISA Kit (AB283879)

Interpolated concentrations of spike Noggin in human serum, plasma samples.

The concentrations of Noggin were measured in duplicates, interpolated from the Noggin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma - citrate 50%, plasma - EDTA 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Noggin ELISA Kit (AB283879)

Native Noggin detected in serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Noggin concentration was determined to be 99 pg/mL with a range of 30 – 260 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Noggin ELISA Kit (AB283879)

Example of human Noggin standard curve in Sample Diluent NS.

The Noggin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 11 pg/mL

Range

54.7 - 3500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Noggin ELISA Kit (ab270218) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Noggin protein in serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Noggin with 11 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Noggin, also known as NOG, is a signaling molecule that regulates the function of bone morphogenic protein. It is known to play a key role in embryonic development, learning, and bone homeostasis. Dysregulation of this protein leads to multiple deformities. The immunogen from which the antibodies in this kit were raised represent the full-length mature protein. This protein is homologous with many species, so cross-reactivity is expected.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]

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Recovery

[ { "sample": "Serum", "range": "80 - 85 %", "average": "= 83" }, { "sample": "EDTA Plasma", "range": "83 - 88 %", "average": "= 85" }, { "sample": "Citrate plasma", "range": "82 - 93 %", "average": "= 88" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Noggin also known as noggin protein is a secreted glycoprotein with a mass of approximately 26 kDa. It antagonizes bone morphogenetic proteins (BMPs) by directly binding them preventing their interaction with receptors. This binding inhibits the signaling pathway which affects cellular processes such as differentiation growth and apoptosis. Noggin expresses prominently in embryonic tissues and in tissues undergoing differentiation particularly in the neural tube notochord and developing articular cartilage. The expression pattern suggests its importance during critical phases of development and tissue patterning.

Biological function summary

Noggin functions as an essential regulator in embryonic development sculpting tissues by inhibiting BMP activity. It does not work alone but functions as part of a complex regulatory network involving other BMP antagonists. By inhibiting BMPs Noggin plays an important role in neural tube closure limb formation and somite development. Noggin's activities help modulate the balance between proliferation and differentiation in these contexts allowing for proper structural formation during embryogenesis.

Pathways

Noggin operates mainly within the TGF-beta/BMP signaling pathway serving as a critical modulator. Inhibiting BMP through Noggin affects downstream targets connecting it to other signaling proteins like SMADs which mediate transcriptional responses. This control enables Noggin to indirectly regulate Wnt signaling as well showing its impact on various developmental stages and processes. Its influence on these pathways indicates Noggin’s importance in maintaining cellular homeostasis during growth and morphogenesis.

Noggin relates to conditions such as fibrodysplasia ossificans progressiva and cartilage-related abnormalities. Mutations or dysregulation of noggin protein can disrupt normal BMP signaling leading to aberrant skeletal development and calcification. This disruption often involves interactions with other proteins like BMP4 revealing Noggin's critical role in maintaining bone and cartilage integrity. Targeting noggin pathway components may provide therapeutic potential for addressing such conditions.

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Product protocols

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Target data

Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Essential for cartilage morphogenesis and joint formation. Inhibits chondrocyte differentiation through its interaction with GDF5 and, probably, GDF6 (PubMed : 21976273, PubMed : 26643732).

See full target information NOG

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human Noggin ELISA Kit

Sandwich ELISA - Human Noggin ELISA Kit (AB283879)

Sandwich ELISA - Human Noggin ELISA Kit (AB283879)

Sandwich ELISA - Human Noggin ELISA Kit (AB283879)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

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Primary Antibodies

Secondary Antibodies

Product promise