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AB326902

Human Noggin ELISA Kit, Chemiluminescent

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Human Noggin ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Noggin with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 5.49 - 12000 pg/ml with a sensitivity of 1.38 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

Noggin, NOG

4 Images
Sandwich ELISA - Human Noggin ELISA Kit, Chemiluminescent (AB326902)
  • sELISA

Lab

Sandwich ELISA - Human Noggin ELISA Kit, Chemiluminescent (AB326902)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Noggin concentration of detected samples was determined to be 18.5 pg/mL with a range of 13.3 - 26.2 pg/mL.

Sandwich ELISA - Human Noggin ELISA Kit, Chemiluminescent (AB326902)
  • sELISA

Supplier Data

Sandwich ELISA - Human Noggin ELISA Kit, Chemiluminescent (AB326902)

Interpolated concentrations of spike Noggin in human serum, plasma samples.

The concentrations of Noggin were measured in duplicates, interpolated from the Noggin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma - citrate 50%, plasma - EDTA 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human Noggin ELISA Kit, Chemiluminescent (AB326902)
  • sELISA

Lab

Sandwich ELISA - Human Noggin ELISA Kit, Chemiluminescent (AB326902)

Dynamic range comparison between the Chemiluminescent Human Noggin SimpleStep ELISA, colorimetric Human Noggin SimpleStep ELISA (ab283879), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human Noggin ELISA Kit, Chemiluminescent (AB326902)
  • sELISA

Lab

Sandwich ELISA - Human Noggin ELISA Kit, Chemiluminescent (AB326902)

Standard curve comparison between the Chemiluminescent Human Noggin SimpleStep ELISA and colorimetric Human Noggin SimpleStep ELISA (ab283879) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Citrate plasma

Reacts with

Human, Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.38 pg/mL

Range

5.49 - 12000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human Noggin ELISA Kit, Chemiluminescent (ab326902) is a rapid single-wash sandwich 55-min ELISA to measure Human Noggin in Serum, EDTA Plasma, Citrate plasma with a sensitivity of 1.38 pg/mL.

How the assay works

Human Noggin ELISA Kit, Chemiluminescent (ab326902) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Noggin (ab326902) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-NOG antibody [EPR23385-256] - BSA and Aside free (Capture) ab281161
- Anti-NOG antibody [EPR23385-234] - BSA and Aside free (Detector) ab281011
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.9" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 8.3" } ]
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Recovery

[ { "sample": "Serum", "range": "80 - 85 %", "average": "= 85" }, { "sample": "EDTA Plasma", "range": "83 - 88 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "82 - 93 %", "average": "= 93" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Essential for cartilage morphogenesis and joint formation. Inhibits chondrocyte differentiation through its interaction with GDF5 and, probably, GDF6 (PubMed : 21976273, PubMed : 26643732).
See full target information NOG
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com