Human Nogo Receptor ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Nogo Receptor in Human in Plasma, Serum, Cell culture supernatant samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.85 - 200 ng/mL
- Sensitivity
- = 0.85 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor for RTN4, OMG and MAG (PubMed:12037567, PubMed:12068310, PubMed:12089450, PubMed:12426574, PubMed:12839991, PubMed:16712417, PubMed:18411262, PubMed:19052207). Functions as a receptor for the sialylated gangliosides GT1b and GM1 (PubMed:18411262). Besides, functions as a receptor for chondroitin sulfate proteoglycans (By similarity). Can also bind heparin (By similarity). Intracellular signaling cascades are triggered via the coreceptor NGFR (PubMed:12426574). Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton (PubMed:16712417, PubMed:22325200). Mediates axonal growth inhibition (PubMed:12839991, PubMed:19052207, PubMed:28892071). Plays a role in regulating axon regeneration and neuronal plasticity in the adult central nervous system. Plays a role in postnatal brain development. Required for normal axon migration across the brain midline and normal formation of the corpus callosum. Protects motoneurons against apoptosis; protection against apoptosis is probably mediated via interaction with MAG. Acts in conjunction with RTN4 and LINGO1 in regulating neuronal precursor cell motility during cortical development. Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development (PubMed:22325200).
Alternative names
NOGOR, UNQ330/PRO526, RTN4R, Reticulon-4 receptor, Nogo receptor, Nogo-66 receptor, NgR
What's included?
Recommended products
Human Nogo Receptor ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Nogo Receptor in Human in Plasma, Serum, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.85 - 200 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.85 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture media | Average % = 90.81 | Range 79 - 105 % |
Sample type Plasma | Average % = 71.38 | Range 69 - 76 % |
Sample type Serum | Average % = 71.02 | Range 68 - 75 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human Nogo Receptor ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD42b in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Nogo Receptor coated on a 96-well plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Nogo Receptor also known as Reticulon 4 receptor (RTN4R) is a protein that functions as an axon growth inhibitor. This receptor with an approximate molecular mass of 55 kDa binds to myelin-associated inhibitors to regulate neuronal growth and regeneration. It is part of the Nogo signaling pathway and is expressed mainly in the central nervous system. The receptor interacts with ligands like Nogo-A MAG and OMgp which play key roles in the restriction of axonal regeneration.
- Biological function summary
Nogo Receptor plays a critical role in the regulation of neural plasticity and axonal growth. The receptor forms a complex with co-receptors such as p75 neurotrophin receptor (p75NTR) and LINGO-1 and this complex significantly influences signal transduction. Nogo Receptor-mediated signaling restricts the ability of neurons to regrow or extend after injury maintaining inhibitory influence on the nervous system regeneration.
- Pathways
This receptor actively participates in neural inhibitory signaling and axon guidance pathways. It integrates signals from proteins like Nogo-A and glial inhibitors to regulate neuron morphology and network architecture. Understanding these pathways reveals Nogo Receptor's involvement in neuronal development and injury responses associating with signaling proteins like ROCK that mediate structural rearrangement of the cytoskeleton.
- Associated diseases and disorders
Nogo Receptor has relevance particularly in central nervous system injuries and multiple sclerosis (MS). Its high expression in the CNS aligns with limited neural repair and recovery in these conditions. Nogo Receptor signaling inhibits axonal regeneration contributing to the pathology of diseases like spinal cord injury and MS. In multiple sclerosis interactions between Nogo Receptor and MAG or OMgp correspond with the restricted repair and remyelination processes observed in demyelinating lesions.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Sandwich ELISA - Human Nogo Receptor ELISA Kit (ab309415)
Example of standard curve using ab309415. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com