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AB309415

Human Nogo Receptor ELISA Kit

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Human Nogo Receptor ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Nogo Receptor in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

NOGOR, UNQ330/PRO526, RTN4R, Reticulon-4 receptor, Nogo receptor, Nogo-66 receptor, NgR

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Sandwich ELISA - Human Nogo Receptor ELISA Kit (AB309415)
  • sELISA

Supplier Data

Sandwich ELISA - Human Nogo Receptor ELISA Kit (AB309415)

Example of standard curve using ab309415. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.85 ng/mL

Range

0.85 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Nogo Receptor ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD42b in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Nogo Receptor coated on a 96-well plate.
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Recovery

[ { "sample": "Cell culture media", "range": "79 - 105 %", "average": "= 90.81" }, { "sample": "Plasma", "range": "69 - 76 %", "average": "= 71.38" }, { "sample": "Serum", "range": "68 - 75 %", "average": "= 71.02" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309415-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309415-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309415-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Nogo Receptor/Reticulon-4 Receptor Antibody-coated ELISA Plate", "number":"AB309415-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Nogo Receptor/Reticulon-4 Receptor Detection Antibody", "number":"AB309415-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB309415-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309415-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309415-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Nogo Receptor/Reticulon-4 Receptor Protein Standard", "number":"AB309415-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Nogo Receptor also known as Reticulon 4 receptor (RTN4R) is a protein that functions as an axon growth inhibitor. This receptor with an approximate molecular mass of 55 kDa binds to myelin-associated inhibitors to regulate neuronal growth and regeneration. It is part of the Nogo signaling pathway and is expressed mainly in the central nervous system. The receptor interacts with ligands like Nogo-A MAG and OMgp which play key roles in the restriction of axonal regeneration.
Biological function summary

Nogo Receptor plays a critical role in the regulation of neural plasticity and axonal growth. The receptor forms a complex with co-receptors such as p75 neurotrophin receptor (p75NTR) and LINGO-1 and this complex significantly influences signal transduction. Nogo Receptor-mediated signaling restricts the ability of neurons to regrow or extend after injury maintaining inhibitory influence on the nervous system regeneration.

Pathways

This receptor actively participates in neural inhibitory signaling and axon guidance pathways. It integrates signals from proteins like Nogo-A and glial inhibitors to regulate neuron morphology and network architecture. Understanding these pathways reveals Nogo Receptor's involvement in neuronal development and injury responses associating with signaling proteins like ROCK that mediate structural rearrangement of the cytoskeleton.

Nogo Receptor has relevance particularly in central nervous system injuries and multiple sclerosis (MS). Its high expression in the CNS aligns with limited neural repair and recovery in these conditions. Nogo Receptor signaling inhibits axonal regeneration contributing to the pathology of diseases like spinal cord injury and MS. In multiple sclerosis interactions between Nogo Receptor and MAG or OMgp correspond with the restricted repair and remyelination processes observed in demyelinating lesions.
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Product protocols

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Target data

Receptor for RTN4, OMG and MAG (PubMed : 12037567, PubMed : 12068310, PubMed : 12089450, PubMed : 12426574, PubMed : 12839991, PubMed : 16712417, PubMed : 18411262, PubMed : 19052207). Functions as a receptor for the sialylated gangliosides GT1b and GM1 (PubMed : 18411262). Besides, functions as a receptor for chondroitin sulfate proteoglycans (By similarity). Can also bind heparin (By similarity). Intracellular signaling cascades are triggered via the coreceptor NGFR (PubMed : 12426574). Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton (PubMed : 16712417, PubMed : 22325200). Mediates axonal growth inhibition (PubMed : 12839991, PubMed : 19052207, PubMed : 28892071). Plays a role in regulating axon regeneration and neuronal plasticity in the adult central nervous system. Plays a role in postnatal brain development. Required for normal axon migration across the brain midline and normal formation of the corpus callosum. Protects motoneurons against apoptosis; protection against apoptosis is probably mediated via interaction with MAG. Acts in conjunction with RTN4 and LINGO1 in regulating neuronal precursor cell motility during cortical development. Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development (PubMed : 22325200).
See full target information RTN4R
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