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AB155437

Human Notch-1 ELISA Kit (TAN-1)

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Human Notch-1 ELISA Kit (TAN-1) is a Sandwich (quantitative) ELISA for the measurement of Human Notch-1 (TAN-1) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

TAN1, NOTCH1, Neurogenic locus notch homolog protein 1, Notch 1, hN1, Translocation-associated notch protein TAN-1

2 Images
Sandwich ELISA - Human Notch-1 ELISA Kit (TAN-1) (AB155437)
  • sELISA

Unknown

Sandwich ELISA - Human Notch-1 ELISA Kit (TAN-1) (AB155437)

Representative standard curve using ab155437 - Assay Diluent B.

Sandwich ELISA - Human Notch-1 ELISA Kit (TAN-1) (AB155437)
  • sELISA

Unknown

Sandwich ELISA - Human Notch-1 ELISA Kit (TAN-1) (AB155437)

Representative standard curve using ab155437 - Assay Diluent A.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 20 pg/mL

Range

0.029 - 7 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Notch-1 (TAN-1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human Notch-1 (TAN-1) in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human Notch-1(TAN-1) coated on a 96-well plate. Standards and samples are pipetted into the wells and Notch-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated Anti-Human Notch-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Notch-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

This kit can detect extracellular domain of NOTCH1.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Cell culture media", "range": "93 - 113 %", "average": "= 103.1" }, { "sample": "Plasma", "range": "86 - 102 %", "average": "= 93.78" }, { "sample": "Serum", "range": "88 - 102 %", "average": "= 96.06" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155437-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155437-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB155437-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155437-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Notch-1", "number":"AB155437-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB155437-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Notch-1 Microplate (12 x 8 wells)", "number":"AB155437-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155437-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human Notch-1 Standard (lyophilized)", "number":"AB155437-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155437-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Notch-1 also known as TAN-1 is a transmembrane receptor protein with a molecular mass of approximately 300 kDa. It is part of the Notch family and is extensively expressed in various tissues including the brain heart and immune cells. The receptor plays an important role in cell-fate determination by mediating communication between adjacent cells through its interaction with notch ligands like Delta and Jagged.
Biological function summary

Notch-1 directly influences cell differentiation proliferation and apoptosis by participating in the Notch signaling pathway. It is part of a complex with Notch ligands and the γ-secretase enzyme which facilitates its cleavage and activation. This signaling assists in controlling the balance between cell types in the developing organism and adult tissue homeostasis making it important for proper function and organism development.

Pathways

Notch-1 is involved in regulating the Notch signaling pathway which impacts gene expression once the intracellular domain translocates to the nucleus. Additionally it interacts with proteins such as Mastermind-like proteins to activate transcription of target genes. Notch-1 also intersects with the Wnt signaling pathway suggesting crosstalk between these important pathways in maintaining cell fate decisions and tissue development.

Notch-1 has connections to T-cell acute lymphoblastic leukemia as mutations in Notch-1 often lead to uncontrolled cell proliferation. The receptor is also linked to breast cancer where abnormal Notch-1 signaling encourages tumor progression. In these disorders Notch-1 interacts with proteins such as NF-kB which influences cancer cell survival and proliferation making Notch-1 a significant target for therapeutic interventions.
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Product protocols

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Target data

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).
See full target information NOTCH1
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websiteProtocolBooklet
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