Human NOTUM ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human NOTUM - Intracellular in Cell Lysate, Tissue Extracts samples.
Images
Key facts
- Detection method
Colorimetric
- Sample types
Cell Lysate, Tissue Extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
46.875 - 3000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 17.047 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors (PubMed:25731175).
Alternative names
Palmitoleoyl-protein carboxylesterase NOTUM, hNOTUM, NOTUM, OK/SW-CL.30
What's included?
Recommended products
Human NOTUM ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human NOTUM - Intracellular in Cell Lysate, Tissue Extracts samples.
Alternative names
Palmitoleoyl-protein carboxylesterase NOTUM, hNOTUM, NOTUM, OK/SW-CL.30
Key facts
- Detection method
Colorimetric
- Sample types
Cell Lysate, Tissue Extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
46.875 - 3000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 17.047 pg/mL
- General recovery
105%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 3.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 7.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Tissue Extracts | Average % = 100 | Range 85 - 110 % |
Sample type Cell Lysate | Average % = 105 | Range 95 - 117 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human NOTUM SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NOTUM protein in Cell Lysate, Tissue extracts. Quantitate Human NOTUM with 17.047 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NOTUM is a lipase-like enzyme that functions mechanically to remove lipid modifications specifically palmitoleate from the Wnt proteins. It is alternatively known as palmitoleoyl-protein carboxylesterase. The molecular mass of NOTUM is approximately 53 kDa. NOTUM is expressed mainly in tissues such as the liver kidneys and gut where it plays a role in regulating signaling pathways.
- Biological function summary
NOTUM inhibits the Wnt signaling pathway by cleaving the lipid moiety attached to Wnt proteins making it inactive. NOTUM acts independently and is not part of larger protein complexes. This regulation of Wnt signaling is important for maintaining cellular processes such as cell proliferation differentiation and migration especially during development and tissue homeostasis.
- Pathways
NOTUM primarily influences the Wnt signaling pathway. This pathway is significant for embryonic development and adult homeostasis. By inactivating Wnt proteins NOTUM indirectly affects other pathway components like β-catenin which is an important mediator in the Wnt pathway. NOTUM ensures the balance of Wnt signaling preventing overactivation that could lead to abnormal cell growth.
- Associated diseases and disorders
NOTUM's role in regulating Wnt signaling links it to conditions such as colorectal cancer and osteoporosis. In cancers overexpression of NOTUM can contribute to Wnt pathway dysregulation while its inhibition may present a therapeutic angle. In osteoporosis alterations in NOTUM activity can affect bone formation processes through its influence on Wnt-related proteins like LRP5/6. Understanding NOTUM's interactions in these diseases may provide insights into novel treatment strategies.
Product promise
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3 product images
Sandwich ELISA - Human NOTUM ELISA Kit- Intracellular (ab317780)
Example of human NOTUM standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human NOTUM ELISA Kit- Intracellular (ab317780)
Interpolated concentrations of human NOTUM in HepG2 Extract, Placenta Extract. Interpolated concentration of native NOTUM was measured in duplicate at different sample concentrations. Undiluted samples are as follows: HepG2 Extract 250 ug/mL and Placenta Extract 500 ug/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human NOTUM ELISA Kit- Intracellular (ab317780)
Example of human NOTUM standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
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