AB317780

Human NOTUM ELISA Kit- Intracellular

SimpleStep
Recombinant
What is this?

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Human NOTUM ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human NOTUM with a sensitivity of 17.047 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

OK/SW-CL.30, NOTUM, Palmitoleoyl-protein carboxylesterase NOTUM, hNOTUM

3 Images

Sandwich ELISA - Human NOTUM ELISA Kit- Intracellular (AB317780)

sELISA

Supplier Data

Sandwich ELISA - Human NOTUM ELISA Kit- Intracellular (AB317780)

Example of human NOTUM standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human NOTUM ELISA Kit- Intracellular (AB317780)

Interpolated concentrations of human NOTUM in HepG2 Extract, Placenta Extract. Interpolated concentration of native NOTUM was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HepG2 Extract 250 ug/mL and Placenta Extract 500 ug/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human NOTUM ELISA Kit- Intracellular (AB317780)

Example of human NOTUM standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 17.047 pg/mL

Range

46.875 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human NOTUM SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NOTUM protein in Cell Lysate, Tissue extracts. Quantitate Human NOTUM with 17.047 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.3" } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": "85 - 110 %", "average": "= 100" }, { "sample": "Cell Lysate", "range": "95 - 117 %", "average": "= 105" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab317780-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB317780-CMP04" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB317780-CMP03" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317780-CMP12" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB317780-CMP10" }, { "size":"20 x 1 Vial", "name":"Human NOTUM Lyophilized Recombinant Protein", "number":"AB317780-CMP09" }, { "size":"10 x 600 µL", "name":"10X Human NOTUM Capture Antibody", "number":"AB317780-CMP01" }, { "size":"10 x 600 µL", "name":"10X Human NOTUM Detector Antibody", "number":"AB317780-CMP08" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317780-CMP05" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB317780-CMP11" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB317780-CMP06" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab317780-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB317780-CMP04" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB317780-CMP03" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317780-CMP12" }, { "size":"2 x 1 Vial", "name":"Human NOTUM Lyophilized Recombinant Protein", "number":"AB317780-CMP09" }, { "size":"1 x 600 µL", "name":"10X Human NOTUM Capture Antibody", "number":"AB317780-CMP01" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB317780-CMP10" }, { "size":"1 x 600 µL", "name":"10X Human NOTUM Detector Antibody", "number":"AB317780-CMP08" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB317780-CMP07" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB317780-CMP11" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB317780-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab317780-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB317780-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB317780-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB317780-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human NOTUM Lyophilized Recombinant Protein", "number":"AB317780-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317780-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human NOTUM Capture Antibody", "number":"AB317780-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB317780-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human NOTUM Detector Antibody", "number":"AB317780-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317780-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB317780-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB317780-CMP06", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOTUM is a lipase-like enzyme that functions mechanically to remove lipid modifications specifically palmitoleate from the Wnt proteins. It is alternatively known as palmitoleoyl-protein carboxylesterase. The molecular mass of NOTUM is approximately 53 kDa. NOTUM is expressed mainly in tissues such as the liver kidneys and gut where it plays a role in regulating signaling pathways.

Biological function summary

NOTUM inhibits the Wnt signaling pathway by cleaving the lipid moiety attached to Wnt proteins making it inactive. NOTUM acts independently and is not part of larger protein complexes. This regulation of Wnt signaling is important for maintaining cellular processes such as cell proliferation differentiation and migration especially during development and tissue homeostasis.

Pathways

NOTUM primarily influences the Wnt signaling pathway. This pathway is significant for embryonic development and adult homeostasis. By inactivating Wnt proteins NOTUM indirectly affects other pathway components like β-catenin which is an important mediator in the Wnt pathway. NOTUM ensures the balance of Wnt signaling preventing overactivation that could lead to abnormal cell growth.

NOTUM's role in regulating Wnt signaling links it to conditions such as colorectal cancer and osteoporosis. In cancers overexpression of NOTUM can contribute to Wnt pathway dysregulation while its inhibition may present a therapeutic angle. In osteoporosis alterations in NOTUM activity can affect bone formation processes through its influence on Wnt-related proteins like LRP5/6. Understanding NOTUM's interactions in these diseases may provide insights into novel treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors (PubMed : 25731175).

See full target information NOTUM

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com