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AB327290

Human NOX2/gp91phox ELISA Kit, Chemiluminescent - Intracellular

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Human NOX2/gp91phox ELISA Kit, Chemiluminescent - Intracellular is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human NOX2/gp91phox with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 1.1 - 2400 pg/ml with a sensitivity of 0.34 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
3 Images
Sandwich ELISA - Human NOX2/gp91phox ELISA Kit, Chemiluminescent - Intracellular (AB327290)
  • sELISA

Supplier Data

Sandwich ELISA - Human NOX2/gp91phox ELISA Kit, Chemiluminescent - Intracellular (AB327290)

Interpolated concentration of native NOX2/gp91phox was measured in duplicate at different sample concentrations. Undiluted samples are as follows : PBMC cell extract 145 µg/mL and HL-60 cell extract 250 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human NOX2/gp91phox ELISA Kit, Chemiluminescent - Intracellular (AB327290)
  • sELISA

Lab

Sandwich ELISA - Human NOX2/gp91phox ELISA Kit, Chemiluminescent - Intracellular (AB327290)

Standard curve comparison between the Chemiluminescent Human NOX2/gp91phox SimpleStep ELISA and colorimetric Human NOX2/gp91phox SimpleStep ELISA (ab300334) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human NOX2/gp91phox ELISA Kit, Chemiluminescent - Intracellular (AB327290)
  • sELISA

Lab

Sandwich ELISA - Human NOX2/gp91phox ELISA Kit, Chemiluminescent - Intracellular (AB327290)

Dynamic range comparison between the Chemiluminescent Human NOX2/gp91phox SimpleStep ELISA, colorimetric Human NOX2/gp91phox SimpleStep ELISA (ab300334), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Key facts

Detection method

Luminescent

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.34 pg/mL

Range

1.1 - 2400 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human NOX2/gp91phox ELISA Kit, Chemiluminescent - Intracellular (ab327290) is a rapid single-wash sandwich 55-min ELISA to measure Human NOX2/gp91phox in Cell Lysate with a sensitivity of 0.34 pg/mL.

How the assay works

Human NOX2/gp91phox ELISA Kit, Chemiluminescent - Intracellular (ab327290) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human NOX2/gp91phox (ab327290) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-NOX2/gp91phox antibody [EPR24537-56] - BSA and Aside free (Capture) ab300696
- Anti-NOX2/gp91phox antibody [EPR24537-21] - BSA and Aside free (Detector) ab300697

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.4" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "98 - 107 %", "average": "= 101" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Catalytic subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (PubMed : 15338276, PubMed : 36241643, PubMed : 36413210, PubMed : 38355798). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction (Probable) (PubMed : 38355798). Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane (PubMed : 19028840, PubMed : 38355798). This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox (By similarity). NADPH oxidase complex assembly is impaired through interaction with NRROS (By similarity).
See full target information CYBB

Alternative Names

NOX2, CYBB, NADPH oxidase 2, CGD91-phox, Cytochrome b(558) subunit beta, Cytochrome b-245 heavy chain, Heme-binding membrane glycoprotein gp91phox, Neutrophil cytochrome b 91 kDa polypeptide, Superoxide-generating NADPH oxidase heavy chain subunit, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, Cytochrome b558 subunit beta
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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