JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB300335

Human NOX2/gp91phox ELISA Kit - Extracellular

Be the first to review this product! Submit a review

|

(0 Publication)

Human NOX2/gp91phox ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Human NOX2/gp91phox with a sensitivity of 1.75 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

NOX2, CYBB, Cytochrome b-245 heavy chain, CGD91-phox, Cytochrome b(558) subunit beta, Heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, Neutrophil cytochrome b 91 kDa polypeptide, Superoxide-generating NADPH oxidase heavy chain subunit, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, Cytochrome b558 subunit beta

2 Images
Sandwich ELISA - Human NOX2/gp91phox ELISA Kit - Extracellular (AB300335)
  • sELISA

Supplier Data

Sandwich ELISA - Human NOX2/gp91phox ELISA Kit - Extracellular (AB300335)

Example of human NOX2/gp91phox standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human NOX2/gp91phox ELISA Kit - Extracellular (AB300335)
  • sELISA

Supplier Data

Sandwich ELISA - Human NOX2/gp91phox ELISA Kit - Extracellular (AB300335)

Recombinant NOX2/gp91phox was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are as follows : plasma (citrate) 50%, plasma (EDTA) 25%, and 1640 RPMI cell culture media 50%. The interpolated values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, EDTA Plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.75 pg/mL

Range

6.25 - 400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human NOX2/gp91phox SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NOX2/gp91phox protein in human plasma (citrate), plasma (EDTA), and cell culture supernatant. Quantitate Human NOX2/gp91phox with 1.75 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" } ]
style
left-column

Recovery

[ { "sample": "Citrate plasma", "range": "81 - 86 %", "average": "= 83" }, { "sample": "EDTA Plasma", "range": "82 - 93 %", "average": "= 89" }, { "sample": "Cell culture media", "range": "82 - 94 %", "average": "= 89" } ]
style
left-column

What's included?

{ "values": { "96Tests": { "sellingSize": "96 Tests", "publicAssetCode":"ab300335-96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB300335-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB300335-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB300335-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB300335-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB300335-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human NOX2/gp91phox Lyophilized Recombinant Protein", "number":"AB300335-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human NOX2/gp91phox Capture Antibody", "number":"AB300335-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human NOX2/gp91phox Detector Antibody", "number":"AB300335-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB300335-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB300335-CMP10", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOX2 also known as gp91phox or cybb is a membrane-bound protein that plays an important role in the production of reactive oxygen species (ROS). This protein functions as a catalytic component of the NADPH oxidase complex which is primarily found in phagocytes like neutrophils and macrophages. The NOX2 protein has a molecular weight of approximately 91 kDa. It is expressed prominently in the cell membranes of these immune cells where it participates in the body's defense mechanisms.
Biological function summary

NOX2 participates in the generation of superoxide by using NADPH as a substrate an essential function performed by the multi-subunit NADPH oxidase complex. This process helps in the formation of microbicidal ROS which neutrophils and macrophages require for killing pathogens. The gp91phox component NOX2's alternate name partners with p22phox and other cytosolic subunits like p47phox and p67phox to form the fully active enzyme complex. This emphasizes NOX2's role in the immune system's oxidative burst an important antimicrobial response.

Pathways

NOX2 is an integral part of the oxidative burst pathway important in host defense. Its activity connects with the MAPK signaling pathway which can be activated by the presence of ROS produced by NOX2. This pathway involves proteins such as ERK JNK and p38 MAPKs which are related through the signaling events that lead to diverse cellular responses including inflammation and stress responses.

NOX2 dysregulation is associated with chronic granulomatous disease (CGD) a condition characterized by a compromised ability of phagocytes to produce ROS leading to recurrent infections. Additionally NOX2 activity links with cardiovascular diseases where its overproduction of ROS can cause oxidative stress contributing to atherosclerosis. The functioning of p22phox another protein in the NADPH oxidase complex becomes particularly relevant in these contexts as it partners with NOX2 to generate the harmful superoxide in such diseases.
style
left-column

Product protocols

style
left-column

Target data

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
See full target information CYBB
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Primary Antibodies

AB310337

Anti-NOX2/gp91phox antibody [EPR28415-13]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] (AB310337)

  • 0
  • 0
Proteins & Peptides

AB114183

Recombinant Human NOX2/gp91phox protein

SDS-PAGE - Recombinant Human NOX2/gp91phox protein (AB114183)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com